tbxas1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGCGAGCCACCACCGTCA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAAGAGCCCACCTTA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714719
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1605nts
Open reading frame: 1 to 1605

ATGGAAGAGCCCACCTTATGGGGACTGGATGGGTGCACAGTGACCTTCGCTCTTGTTGCT
GGGTTCCTGGGCCTTCTCTACTGGTACTCCGTGTCTGCATTCTCACAGCTGGATAAAGTT
GGGATAAAGCATCCGAAACCTCTGCCGTTCATTGGTAACGTCATGCTCTTCAAAAAGGGA
TTTTGGGAAGGAGACAGACATCTTATAAAAACGTATGGTCCCATTTGTGGATATTACATG
GGGAGGCGCCCGATGATTGTCATAGCTGAACCAGATGCCATTAAACAGGTTCTGCAGAAA
GATTTTGTTAACTTCACTAACCGAATGAAACTCAATTTAGTGACTAAGCCAATGAGTGAC
AGCCTGCTGTGCCTCCGAGACGACAAGTGGAAGCGTGTGCGCAGTGTGATGACTCCATCT
TTCAGCGCCATACGAATGAAAGAGATGTGCCCTCTAATAAACCAATGCTGTGATGTGTTA
GTGGATAATCTGCTAGAGTACGCCAGTTCTGGAGAGGCATGCAATGTACAAAGGTGCTAT
GCCTGCTATACCATGGATGTGGTTGCCAGTGTAGCATTCGGCACTCAGGTGGATTCTCAG
AGAGACCCCGACCATCCCTTGGTGCAGAACTGCAAAAGATTCCTGGAACTATTTACCCCT
TTCAAACCCCTTATCTTGCTGTGCTTGGCGTTTCCGTCTATCATGATCCCCATTGCACGC
CGGCTTCCCAATAAACAGCGAGACAGAATAAACAGCTTCTTCCTCAAGGTCATTAGGGAC
ATCATCGCTTTCCGAGAGAATCAGCCGCCTAACGAGAGACGCCGGGACTTCTTGCAGCTC
ATGTTGGACGCTCAAGACTCGGTCAGCCACGTGACAGTGGATCACTTTGACATAGTCAAT
CAGGCGGATCTCTCGGTGCCGCAGAACAGCCCCTCGGAGAAGCAGGACCGGGGTCAGGAT
CCACCTAGAAAATCTAGCAAAAAGCTGAACAAAGAGGAGATACTGGGACAGGCCTTCATC
TTCCTCATCGCCGGATACGAGACCACTTGCAGCCTCCTCTCTTTTACCTCCTACCTGTTG
GCCACTCACCCGGACTGTCAGGAGAAGCTGCTCAAGGAGGTGGACGAGTTTAGCCAAGAG
CATAAAGAAGCCGACTACAATACGGTGCATGACCTGCCCTATATGGACATGGTGATTAAT
GAAACCCTGCGAATGTACCCGCCAGCATACAGATTTGCCAGGGAAGCAGCCCGAGATTGC
ACCGTGATGGGGCAGAATATCCCGGCTGGCGCTGTGGTGGAAATTCCCATCGGCTGCTTG
CAGAATGATCCGCGATTTTGGCACGAGCCGGAGAAATTTAATCCTGAGAGATTTACAGCA
GAAGAAAAGCAGAAGCGCCACCCATTTTTGTTCCTCCCGTTTGGAGCCGGGCCCCGGAGC
TGCATCGGGATGAGACTCGCCCTACTGGAAGCCAAGATCACCCTGTACAGAGTCTTGCAG
AAGTTCCGCTTCCAGACATGTGACCTTACCCAGATCCCTCTCCAGCTCAGTGCAATGTCC
ACACTGAGACCTAAGGACGGTGTGTATGTGACGGTGGTGGCTCGCTAG

Protein sequence predicted based on nucleotide sequence

MEEPTLWGLDGCTVTFALVAGFLGLLYWYSVSAFSQLDKVGIKHPKPLPFIGNVMLFKKG
FWEGDRHLIKTYGPICGYYMGRRPMIVIAEPDAIKQVLQKDFVNFTNRMKLNLVTKPMSD
SLLCLRDDKWKRVRSVMTPSFSAIRMKEMCPLINQCCDVLVDNLLEYASSGEACNVQRCY
ACYTMDVVASVAFGTQVDSQRDPDHPLVQNCKRFLELFTPFKPLILLCLAFPSIMIPIAR
RLPNKQRDRINSFFLKVIRDIIAFRENQPPNERRRDFLQLMLDAQDSVSHVTVDHFDIVN
QADLSVPQNSPSEKQDRGQDPPRKSSKKLNKEEILGQAFIFLIAGYETTCSLLSFTSYLL
ATHPDCQEKLLKEVDEFSQEHKEADYNTVHDLPYMDMVINETLRMYPPAYRFAREAARDC
TVMGQNIPAGAVVEIPIGCLQNDPRFWHEPEKFNPERFTAEEKQKRHPFLFLPFGAGPRS
CIGMRLALLEAKITLYRVLQKFRFQTCDLTQIPLQLSAMSTLRPKDGVYVTVVAR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable