Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATGGGGTAATTCAGCACCACGTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGACACTGACTTGTACGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714781
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2922nts
Open reading frame: 1 to 2922
ATGGACACTGACTTGTACGATGAGTTTGGTAACTACATTGGGCCTGAATTGGACTCGGAT
GATGAAAATGATGACGATGACATGGGGAGAGACGACAGGGACGTGGATGTGGCAGAAGAT
GAGGATGATGAAGAATACATGGGGGATCCTGACGAGGACCGTGGAGGAATGGAGGTTGTG
CTGCATGAAGACAAAAAATATTATCCCACGGCAGAAGAAATTTATGGGCCAGAGGTGGAA
ACCATTGTGCAAGAGGAAGACACCCAGCCACTTACTGAGCCAATTATTAAACCAGTGAAG
GCTAAAAAGTTTTCTGTGATGGAGCAGGCACTGCCAGCAACTGTTTATGAGATGGACTTC
CTTGCGGATCTAATGGACAATCCTGAGCTGATCCGGAATGTGACTCTGTGTGGCCACCTG
CACCATGGAAAGACCTGTTTTGTGGACTGTTTAATTGAGCAAACGCACCCTGAGATCCGC
AAGCGCCATGACCAAGATCTTTGCTACACAGATATTCTATTTACTGAGCAGGAGAGAGGA
GTTGGTATGAAGAGTACCCCAGTGACAATTGTGCTTCCAGACACCAAGGAGAAGTCTTAC
CTGTTTAATATTATGGACACACCAGGCCATGTTAACTTTTCAGATGAAGTTACAGCTGGA
CTTCGGATCTCTGATGGAGTGGTGCTGTTCATCGATGCAGCTGAGGGGGTTATGCTGAAC
ACAGAGAGATTGATTAAACACGCTGTCCAGGAACGTCTGGCCGTCACTGTCTGCATCAAC
AAGATAGACAGGCTGATTCTCGAGCTCAAGCTGCCTCCCACTGATGCTTATTACAAACTG
CGGCACATTGTGGATGAAGTGAACGGGCTGCTGAGCGTGTATTCCACCGACGAGAACCTG
GTTCTCTCACCTCTCCTGGGAAACGTCTGTTTCGCTAGCTCGCAGTACAGTATCTGCTTT
ACACTGGGCTCTTTTGCCAAAATTTATGCCGACACATACGGGGACATCAACTACCAGGAA
TTTGCAAAGCGCTTGTGGGGCGACATCTACTTCAATCCTAAAACCCGCAAATTCACCAAG
AAAGCTCCTACCAGCAGCTCACAGCGCAGTTTTGTGGAGTTCGTCCTGGAACCTCTGTAC
AAGATTCTGGCTCAGGTGGTCGGTGATGTAGACACCACTCTGCCGCAGACTCTAGAGGAG
TTGGGAATTCATCTGACCAAAGAGGAACTGAAACTAAACATCCGCCCTCTCCTGAGACTT
GTCTGCAATCGCTTCTTCGGGGAATTCACCGGATTCGTGGATATGTGTGTCCAGCACATC
CCTTCTCCCAAAGCGGGGGCAAGAGCCAAGATTGAACACACCTACACAGGGGGCATTGAT
TCTGAATTGGGAGAGGGCATGAGTGAATGCGACCCAGATGGCCCTCTTATGTGTCATACC
ACCAAGATGTATAGCACAGATGATGGGGTGCAGTTTCGAGCATTTGGGAGGTTACTCAGT
GGAACAATCCATGCTGGACAACCAGTCAAAGTTCTGGGAGAGAACTACACGCTGGAGGAT
GAAGAGGACTCGCAGGTCTGCACTATAGGGAGGCTGTGGGTGTCTGTAGCTAGGTATCAC
GTTGAAGTGAACAGAGTTCCAGCTGGAAACTGGGTGTTGATAGAGGGCGTGGACCAGCCT
ATTGTGAAGACAGCAACCATCACAGAGCCACGGGGCAATGAAGAGGCCCAGATCTTCCGA
CCGCTGAAATTCAACACCACGTCCGTCATTAAAATTGCTGTGGAACCAGTCAACCCCTCA
GAATTGCCCAAGATGTTGGATGGGTTGAGGAAGGTCAACAAGAGCTATCCTTCCCTCACC
ACCAAGGTGGAGGAGTCTGGGGAGCACGTGATCCTTGGCACTGGAGAGCTGTATCTGGAC
TGCGTGATGCACGACCTCCGCAAGATGTACTCAGAGATTGACATTAAGGTGGCGGATCCT
GTGGTTACCTTCTGCGAGACTGTAGTGGAAACCTCATCACTGAAATGTTTTGCTGAGACA
CCAAACAAGAAAAACAAGATCACAATGATAGCGGAGCCGCTGGAGAAAGGATTGGCTGAG
GACATAGAAAATGAAGTGGTGCAGATTTCCTGGAACAGGAAGAAGCTGGGAGAGTTTTTC
CAGACCAAATATGACTGGGATCTGCTCGCTGCCCGTTCTATCTGGGCTTTTGGACCGGAT
ACCACAGGGCCCAACATTCTGGTTGATGACACACTCCCATCTGAGGTGGATAAAGCGTTG
CTGAGTTCAGTCAAAGACAGCATTGTGCAGGGATTTCAGTGGGGAACAAGAGAGGGTCCG
TTGTGTGATGAGTTGATCCGCAATGTCAAATTTAAGATCTTGGATGCGGTTATCGCACAG
GAGCCCCTACACCGCGGTGGGGGACAGATCATCCCTACAGCCCGAAGAGTGGTTTACTCG
GCTTTTCTGATGGCTACTCCCCGTCTAATGGAACCCTATTATTTTGTGGAAGTACAAGCC
CCTGCAGACTGTGTCTCTGCTGTTTACACTGTTTTGGCCCGAAGAAGAGGGCATGTAACT
CAGGACGCCCCAATTCCTGGATCGCCTCTCTACACGATCAAAGCTTTCATCCCAGCAATT
GATTCATTCGGGTTTGAGACTGACCTGCGAACACACACCCAGGGACAAGCCTTCTCCTTG
TCTGTCTTCCATCACTGGCAGATTGTTCCTGGAGACCCTCTGGATAAAAGCATCATTATA
CGACCCTTGGAACCACAACCAGCCCCTCACCTTGCCAGAGAGTTTATGATAAAGACAAGA
CGTAGGAAGGGGCTAAGCGAGGATGTGAGTATCAGCAAATTTTTCGACGACCCTATGTTG
CTGGAACTGGCGAAACAGGACGTGGTGCTGAATTACCCCATGTAA
Protein sequence predicted based on nucleotide sequence
MDTDLYDEFGNYIGPELDSDDENDDDDMGRDDRDVDVAEDEDDEEYMGDPDEDRGGMEVV LHEDKKYYPTAEEIYGPEVETIVQEEDTQPLTEPIIKPVKAKKFSVMEQALPATVYEMDF LADLMDNPELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRHDQDLCYTDILFTEQERG VGMKSTPVTIVLPDTKEKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLN TERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLLSVYSTDENL VLSPLLGNVCFASSQYSICFTLGSFAKIYADTYGDINYQEFAKRLWGDIYFNPKTRKFTK KAPTSSSQRSFVEFVLEPLYKILAQVVGDVDTTLPQTLEELGIHLTKEELKLNIRPLLRL VCNRFFGEFTGFVDMCVQHIPSPKAGARAKIEHTYTGGIDSELGEGMSECDPDGPLMCHT TKMYSTDDGVQFRAFGRLLSGTIHAGQPVKVLGENYTLEDEEDSQVCTIGRLWVSVARYH VEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPS ELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADP VVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQISWNRKKLGEFF QTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLSSVKDSIVQGFQWGTREGP LCDELIRNVKFKILDAVIAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQA PADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSL SVFHHWQIVPGDPLDKSIIIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPML LELAKQDVVLNYPM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
