Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGGGTTACCCTCACTGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCCGAGCGGCTGGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714799
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6090nts
Open reading frame: 1 to 6090
ATGTCCGAGCGGCTGGGGCAGAATGCAAAAGGAAAGGATGGCAAGAAGTATTCGTCCCTC
AACCTATTTGATACTTACAAGGGAAAGTCCTTAGAGGTGCAGAAACCAGCAGTCACCCCC
CGCCATGGCCTCCAAAGTCTCGGTAAAGTTGTTGTTGCCCGGCGTATGCCTCCCCCAGCT
AATCTTCCTAGCCTGAAAGCAGAAAACAAAGGGAATGACCCCAATGTCTCATTAGTACCC
AAAGACGGATCAGGATGGGCAAGCAAGCAGGATACACAAGAGCCAAAGAGTTCCGATGTC
TCTCCACCAGTGCAGCCGGAGTCGCAGCCGCAGCCGGTTATGCAGACGCCTGCATCCAAC
CAGCCGAAACATCCCCCAGTTTCACAAGAGCCCCCACCTCCAGTCGCCGCCGTGGTAAAG
ACCTGGGCACAGGCCAGTGTCACACATGGGTCACAAGGAGATGGTGGAAAGGCATCAAAC
CAACTGTCGCCATTCTCTCGCGAGGAATTTCCAACCCTGCAGGCTGCTGGGGACCAGGAC
AAAAGTGGGCACGACCCGGACACCTCCGCTGTGTCGTATGGGCCAGGACCAAGCCTCCGC
CCCCAAAATGCAACAAATTGGAGGGATGGGGGTGGGAGGGGGGGATTGGCTGTGAGCTCC
CTTGATGGTGAAAGCCGAGAGCCACCTTCAGAAACGGGACACCCCCCCAGCTGTGAGGCA
GCCCCTCGCCCAGGGCTGCCTCAACCCACACCCCAACAATTCCCTCAATATCGGGGGATG
CTGGCACCATTTATGTATCCTCCATACTTGCCGTTTCCCCCTCCCTATGGGCCACAGGGG
GCCTTCCGTTTTCATGGACCAGATGCCCCAAGGTTCCCACGTCTTGCAGCACCTCGTGTC
CCACCAGCAGTTCCCAGAATGCCTCAGCACTTGAATCGTCCGTCCATTTTGAAGCAGGAT
GATCTTAAGGAGTTTGATGAACTTGATCAAGAGGCAGATGATGGCTGGGCAGGAGCACAT
GAAGAGGTTGATTATTCAGAAAAGCTAAAATTTAGTGATGACGAGGATGGGAAAGAGACA
GATGAGGAAGAAAAAAGGGAGACTAAAGGTCTCAATACTGACTTATCTATAACACAACCG
AAGAAGGTGGAAGAGTCTTCAGATGTTAGGAAAGGTGTACCTGCAGAGGAGATGCCCCCA
CCAGCCAAAGTGGCATGGACAGAGGGAAACGGAGGCCAAGAGCCTGTAATGGCAGTTGCT
GCTTCTGCACCCCCTCTTCATAGGACCCAGGCAACCCAAAGAGGCCCACAGCCTGAGCAA
AAGGAGAAACCGACAGTGCCCCAGGCAATGACTTCTCTGGAAGATGAGGATGAGGCATGG
CGCCAGCGCAGGAAGCAGTCCTCCTCTGAGATCTCACAGGCAGTAGAGAGAGCAAGGAGA
CGAAGGGAGGAGGAGGAGCGTAGGATGGAAGATGAAAGGCGAGCTGCTTGTGCTGAGAAA
CTTAAACTTTTGGATATGAAATGCCGCCCTGCACCTGATGAGGTGGAGCCTGCTGTCAGT
ATGAAATGTGGAGGAAATGATGGTGAAGAACTAAGAAGCCAGACTCCTCCAGAAATGGAG
GCCCCACTGTCTGCTGTATCTATACCACATCCTGATGCATCTCCTCCAGTACCTGAAATG
CCTCAAGTAAAAGTAGAGTGTGTTGCCCCACCATCTCGGCCTGAGATTAAACAAGAGTCA
GCACCTCCTGTAAGCCGCCCACCTCCTGCAGGTCCTTCCCAGAGTGGATATGCCAAATTC
CAAAAGAGCCTGCCACCTCGTTTCCAGAGGCAGCAGCAGGAGCAGCTCATGAAGCAGTGG
CAGCAGAGCAGAGACCCTCTCCCTTCAGCTTCAGTTGGACCTCCAACCCAGCAGCCTCCA
GCTCCTCCTTCACAGCCTCCACAGAAAGCGCTTTACAGCGGAGGTTCACTGGGACGGACA
ACCCCAGCACCACAACCACCTCATGGTCCCTTACCTTCTCATGTGAGCTATGATCCTCGC
TGGGTCATGGTGCCACATTTAATTGACCCTCGCCTTATGCAAGGAAGACCCTTGGACTAC
TATACACCCACTGCAGGGGTACACCCTTCAGGGCTGATGACACGAGAACGCTCAGACAGT
GGTGGCTCTGGATCGGATAGGTATGAGCGTCATCCTCCACTGATGAGGGAACGGACAACG
CCACCTACAGACCCTAAACTTTTATGGGGAGGCGAAATGTTTAATTCTCCTGAGGCAAGG
CCTCTAATGTCCTCTATGCGACAAAATATAGAAGATAACAACAGGGAAATAAGAAGTGGC
ACACCTCCTTCTCGCACTGTTGCTTCCCAGAATCTTCAGCAACCCTCTTATTCTGGTAAC
TTCAACGCATATCCAGAAAATTCTGCCTCTGCTCAGCAGACACATGTTGGGCATCATTAT
TCTACAGATGAACAACATACCTGGCACCATCTACACAACCATAAATCTTCACCAACCCCA
CCTCCTCTGTCCAGTGAACAAAAACATCCAGATTCAGCCACTACTCAGACACCCCCTCCA
TCGGCCCCTTCGTCACAGGCCCGCAGACGGAATGATGAGCCTGTCATACTAAGGAGAGAG
GCTTCTGCCGCTCCTCCTTTGCCAGATGCCCCTTCCAAAGAGGAGACAACCAGTATGCGT
CACAAAGAGGACAAAAAGGAAATTGGCAAGGATGAACCAAAACATGAAAGAACCTTGCAA
TCTAGCAGGCCAGATTATCATAGGCCGCCTTCTCGTCGGGAATCCAAAACAGAGACACGA
TGGGGTCCTAGACCTGGTTTAGGGGGAAGTAGGAGGCAAGAAGAAACTGCAGCTACAGGG
GGATCACAGGCTCATACAACAACACAACGTAGGGCAGGGCCTATCAAAAGAGGGGTTAGG
AGGGTGGAAACAATTACCCAAGAGAATAAGACAAGTCCAGCTCCTCCTGCTGTTTCAGAG
ACACAGAAAAATAAACAGGTCAATCTGTCAGATAGGGAGGCAGAGGAAGCAGCAGGATCC
CCATTGAGGAGGAGGAAAGAAATTGCTGGGACCACACTTCCTGCTAGAGAATCTGTAGCT
TTGGTTCCAGCAGGACGTGGCCGTGGAAGAGGAGAGTATTTTTCCCGAGGGCGAGGATTC
CGTGGAGCATATAGCGGACGAAGTAGGGGTGGGAGAGGAAGAAGTCGCGAGTTTCGTGGG
GGCTATAGAAAGGAAAGCCCTTTCTATCGAGAAGATGGAATTGCAGATGGTAAGTCATCT
GGACGTAGAGATGGTCGTGAACGCAACACTAGTGAGACAAGAAGTGAGGGCTCCGAGTAT
GAGGAAGTGCCCAAAAGAAGGCGGCAGCGTGGATCTGAGACTGGAAGTGAGACAGAGCCT
GCCGTTTCTGAAAAGGAGGTGGCCAAGAGTGGGCAATCCCAGCAGCAGAAAGACTCTTTT
AATTCAGTTAGTCAGCATCCACCCGAAAAGGGGAAAGACTCTGGCAGAGGACGTACATTC
ACCCCAAGAGGAGTACCCTCCAGAAGAGGAAGGGGTGGGACATCCATGGTAACCTTTAGT
CCATATCAAAAATATCAGCCACAGCAGCCTATGCTTCCTCCGTCCCAGGCAGCACCACAG
TCTCATAAACCAGAAAGGAAAAATGATAAAGGGGCTTTTACAGCTGTTCCATGTGTAACT
GGAGAGGAGCATCCACAACAGCCACCAAGAAGGAGGCGTCACCATGGTAGATCCCAGCAA
CAAGATAAGCCACCACGGTTTCGGAGGTTGAAACAAGAAAGGGAGAATGCAGCAAGAGCA
GGAAATGGTACAGGAGCACAGGTTCCGCCTCAACAAATTACAGTAGCACAGGAACAGCCC
CAAAGATCCATACAAAATCAAGAGGAACGTACCGTTCCCCGCAGAGGACTTAAGTCCCCA
GAACCTTGCAATGCTAATTCAGACCAGGCAAATGAAGAATGGGAGACTGCATCAGAAAGC
AGTGACTTTACAGAAAAGAAGGAAAGGGGAGAGGTGCCAGTCCAAAATGGCGGCGAATCA
GCACTTTCCTTAAATGTTACATGTGTAAAAGACCAGAAAAGAGATATGTCAAAAAGAAGC
TTCTCAAGCCAGAGACCAGTCATGGAAAGGCAAAATCGTAGACCCATGCACCAAGCTGGC
CAAAGAAATAACAAAGGAGGTGGTAATGGCAGGAGTGGAGACAAGAGAAGCTGGCCATCA
CCTCGCAACAAAGGGGGTAGGTCAAATGAAGACCGTGCTCCTGCCTCGTTACCTCTTCCC
CAGTCAACACCTACGACTAACGTTGTATACCGTGTGGATCACGTGATACATAACCCAGTG
GGGATCCAGCAGGCACTCAGTGAACTTGGTCAGAGGCATAGCAAACGAGCCAGTCATAAC
CTGGATCCATCTCCTGTGCATATTCAACCATCTGCAGATTCCTTTGTTGTCAGCCACAGT
AAACTTCTTAGTGAAACATTAGCTACACCATTACCAAAACGAAATTCACAACTTCGCAGG
GGCAAAGAAGTCACTCATGAGGATATTATTCCACATGGAGCATGCAAGAATGCCACCACT
TGGACAGAGCAAGTTGTTCGAGATCACCAGCCAGAAGCCCTGGCACCACGTGTCTGGAGT
AGAGAGCAGAGCAATGACAGAGATAAGCTGCGCTCTTCATATGCTGTGGAACCATGGATG
GAAAATTTAAATTCCTATGAAGATCCTATAAACACAGAGATGAGTCAGTCAGACAGTGGG
GTTGACCTTAGTAGTGATTCACAGCTTTCTTCAAGCAATAGCAGTCAGCGCAGCTCCCCT
GATGGGGGGTTGAAACCTGAAGAGGCAAATCGGTCAGGTCAAGGAAGAGCAATTGAACCA
CTTCGACATGGACATTCGGTAGTGGTCCACACCCCTGGACCAATTGGAACTGAAAGAATG
CAGAAATCGTCTGCAGATAGTTCTTGTGGCCCATGCAATTCCTCTTCCATTCCTCCCGTT
TCTTGCTCTCCCAGCCCTAAAAGCTATTCCCAAGCAGCTGAAGTTAATAAGCATGGCAGA
GACGCACAATTTACCAAAAGTCTGCAACCAGATGAGACATGTTTCTCAACTCGAGCTCCC
CAACAGCTTACACTTTCAACTGTCACTCCTGAAGAAACAAGACCTCCGCTTCCAGAGGAT
CGGAGTCAGCGATTGTATTCAAACCAGTTCTATCAGACATCCCTTCCTCAGTTGGATGTC
CATGTCAGCAATACAGGCAGTGGCTACAGTGGGTACAGACCTGGGACACCCTCTTTGCAG
CCCTATAGATCTCAGCCTCTGTACCTACATGCTGCTCCATCCCCAGGGTCCTCCACAGCT
CTTTTGCCGGGCTCTGCAGTGCTTTCTGGTATCACTTTGAAAGGGCAGTATATGGAGCTA
AGTGAACTTGGGAAGGTGCCTGCCAGTGGCCTCCTCTACCAGGCACCTGCCTTTCTTTAT
AGTGGCCATTACTGCCCTGCTCAGGTCACCACTGACCAACAACAGCAACTGCTGCAGGTC
AGACAGGATATGGCACCTCCATCTGATTATTACTCTCCTCCAATTCATCACACAGGACAA
AGTGGATATCTGCCACCTGCCCCCACTCAGCAGGTTCTTCTTTCTGTGATGGACTCACAA
CTTCCAATGATGGGGTTTGGCTCACTGTCTTCTGCTCCACAGCAGCCTTCACTGATCACA
ATGGGACAAGCCATGCAACCCATTCATCCTCTTTCTGCAACAGGGCAAACACATTCGCAC
AACATAAGAAATGAGTATCAGACACACTCAGCAGATATAAAGCAATCTGAGGATCACAGG
AGGTCAATGGGTGGATCTGGGCATTGTGTTTCATCTGCAGAAAGAAGGACAAAGCCTTCA
AATTATGGAGTTTTCAGAAATCGCTTTGATGTTTATCAGCAGGCCTATCCTTCAGATGCC
TCCAGATGGACTGCTAGACCATGGGGCAGAGCCCCCCCTCTGGATACTGACTCCAAAAGT
ACCAAAAGCCCCCACAGTGAGGGTAACCCCTAA
Protein sequence predicted based on nucleotide sequence
MSERLGQNAKGKDGKKYSSLNLFDTYKGKSLEVQKPAVTPRHGLQSLGKVVVARRMPPPA NLPSLKAENKGNDPNVSLVPKDGSGWASKQDTQEPKSSDVSPPVQPESQPQPVMQTPASN QPKHPPVSQEPPPPVAAVVKTWAQASVTHGSQGDGGKASNQLSPFSREEFPTLQAAGDQD KSGHDPDTSAVSYGPGPSLRPQNATNWRDGGGRGGLAVSSLDGESREPPSETGHPPSCEA APRPGLPQPTPQQFPQYRGMLAPFMYPPYLPFPPPYGPQGAFRFHGPDAPRFPRLAAPRV PPAVPRMPQHLNRPSILKQDDLKEFDELDQEADDGWAGAHEEVDYSEKLKFSDDEDGKET DEEEKRETKGLNTDLSITQPKKVEESSDVRKGVPAEEMPPPAKVAWTEGNGGQEPVMAVA ASAPPLHRTQATQRGPQPEQKEKPTVPQAMTSLEDEDEAWRQRRKQSSSEISQAVERARR RREEEERRMEDERRAACAEKLKLLDMKCRPAPDEVEPAVSMKCGGNDGEELRSQTPPEME APLSAVSIPHPDASPPVPEMPQVKVECVAPPSRPEIKQESAPPVSRPPPAGPSQSGYAKF QKSLPPRFQRQQQEQLMKQWQQSRDPLPSASVGPPTQQPPAPPSQPPQKALYSGGSLGRT TPAPQPPHGPLPSHVSYDPRWVMVPHLIDPRLMQGRPLDYYTPTAGVHPSGLMTRERSDS GGSGSDRYERHPPLMRERTTPPTDPKLLWGGEMFNSPEARPLMSSMRQNIEDNNREIRSG TPPSRTVASQNLQQPSYSGNFNAYPENSASAQQTHVGHHYSTDEQHTWHHLHNHKSSPTP PPLSSEQKHPDSATTQTPPPSAPSSQARRRNDEPVILRREASAAPPLPDAPSKEETTSMR HKEDKKEIGKDEPKHERTLQSSRPDYHRPPSRRESKTETRWGPRPGLGGSRRQEETAATG GSQAHTTTQRRAGPIKRGVRRVETITQENKTSPAPPAVSETQKNKQVNLSDREAEEAAGS PLRRRKEIAGTTLPARESVALVPAGRGRGRGEYFSRGRGFRGAYSGRSRGGRGRSREFRG GYRKESPFYREDGIADGKSSGRRDGRERNTSETRSEGSEYEEVPKRRRQRGSETGSETEP AVSEKEVAKSGQSQQQKDSFNSVSQHPPEKGKDSGRGRTFTPRGVPSRRGRGGTSMVTFS PYQKYQPQQPMLPPSQAAPQSHKPERKNDKGAFTAVPCVTGEEHPQQPPRRRRHHGRSQQ QDKPPRFRRLKQERENAARAGNGTGAQVPPQQITVAQEQPQRSIQNQEERTVPRRGLKSP EPCNANSDQANEEWETASESSDFTEKKERGEVPVQNGGESALSLNVTCVKDQKRDMSKRS FSSQRPVMERQNRRPMHQAGQRNNKGGGNGRSGDKRSWPSPRNKGGRSNEDRAPASLPLP QSTPTTNVVYRVDHVIHNPVGIQQALSELGQRHSKRASHNLDPSPVHIQPSADSFVVSHS KLLSETLATPLPKRNSQLRRGKEVTHEDIIPHGACKNATTWTEQVVRDHQPEALAPRVWS REQSNDRDKLRSSYAVEPWMENLNSYEDPINTEMSQSDSGVDLSSDSQLSSSNSSQRSSP DGGLKPEEANRSGQGRAIEPLRHGHSVVVHTPGPIGTERMQKSSADSSCGPCNSSSIPPV SCSPSPKSYSQAAEVNKHGRDAQFTKSLQPDETCFSTRAPQQLTLSTVTPEETRPPLPED RSQRLYSNQFYQTSLPQLDVHVSNTGSGYSGYRPGTPSLQPYRSQPLYLHAAPSPGSSTA LLPGSAVLSGITLKGQYMELSELGKVPASGLLYQAPAFLYSGHYCPAQVTTDQQQQLLQV RQDMAPPSDYYSPPIHHTGQSGYLPPAPTQQVLLSVMDSQLPMMGFGSLSSAPQQPSLIT MGQAMQPIHPLSATGQTHSHNIRNEYQTHSADIKQSEDHRRSMGGSGHCVSSAERRTKPS NYGVFRNRFDVYQQAYPSDASRWTARPWGRAPPLDTDSKSTKSPHSEGNP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
