ldb1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ldb1
Gene Name:  LIM domain binding 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTGTGAAGCCTGTGAAGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCTGGATCGAGATGTG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714815
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1125nts
Open reading frame: 1 to 1125

ATGCTGGATCGAGATGTGGGACCCACCCCGATGTATCCACCAACATATTTGGAGCCCGGG
ATAGGGAGGCATACGCCATATGGGAACCAGACAGACTACAGAATATTTGAGCTGAACAAA
CGCTTGCAAAATTGGACAGAGGAATGTGACAATTTGTGGTGGGATGCTTTTACCACAGAG
TTCTTTGAAGATGATGCAATGTTAACCATCACTTTTTGTTTGGAGGATGGACCAAAGAGA
TACACAATTGGACGGACGCTGATCCCGCGGTATTTCCGAAGCATCTTTGAGGGAGGAGCC
ACAGAGCTGTACTATGTCTTGAAACACCCCAAGGAGTCATTCCACAATAATTTTGTTTCC
CTCGACTGTGACCAGTGCACAATGGTCACACAGCATGGCAAACCTATGTTCACACAGGTA
TGTGTAGAAGGCAGATTATACCTGGAGTTCATGTTCGATGACATGATGAGGATAAAAACG
TGGCACTTCAGTATCAGACAACATCGAGAGCTCATTCCCCGGAGTATTCTAGCCATGCAT
GCCCAAGACCCCCAAATGTTGGACCAGTTGTCTAAAAACATTACAAGATGCGGACTTTCC
AACTCCACGCTTAACTACCTCCGACTGTGTGTCATCCTAGAGCCCATGCAGGAGCTTATG
TCCAGGCACAAGACTTACAGTTTAAGTCCTCGGGACTGTCTCAAGACCTGTCTCTTTCAG
AAATGGCAACGCATGGTGGCACCTCCGGCTGAACCAGCCAGACAAGCCCCCAGCAAGCGC
AGGAAAAGGAAAATGTCCGGAGGCAGCACCATGAGTTCTGGAGGGGGCAACACAAATAAC
AGCAACAGCAAAAAGAAGAGCCCAGCCAGTACCTTCGCCCTCTCCAGCCAGGTACCTGAT
GTAATGGTGGTGGGCGAACCAACCCTGATGGGAGGAGAATTCGGAGATGAAGATGAACGG
CTCATAACCCGCCTGGAGAACACTCAGTTTGACGCCGCCAATGGAATTGATGACGAGGAC
AGCTTCAATAACTCGCCAGCACTAGGCGCCAACAGCCCATGGAACAGCAAACCCCCCTCA
AGTCAAGAAAGTAAATCCGAGAACCCAACTTCACAGGCTTCACAGTAA

Protein sequence predicted based on nucleotide sequence

MLDRDVGPTPMYPPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTE
FFEDDAMLTITFCLEDGPKRYTIGRTLIPRYFRSIFEGGATELYYVLKHPKESFHNNFVS
LDCDQCTMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMH
AQDPQMLDQLSKNITRCGLSNSTLNYLRLCVILEPMQELMSRHKTYSLSPRDCLKTCLFQ
KWQRMVAPPAEPARQAPSKRRKRKMSGGSTMSSGGGNTNNSNSKKKSPASTFALSSQVPD
VMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDEDSFNNSPALGANSPWNSKPPS
SQESKSENPTSQASQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable