nono (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATATCGCCGACGTTTGTTG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGGGAAACAGAGGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714841
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1392nts
Open reading frame: 1 to 1392

ATGCAGGGAAACAGAGGGTACAGGATGGAACAGCAGAATCATACACCCCGGAGACAGCAG
CAACAACAACAACACCACCAGCAGCAGCAGCCAGAAACCACTTCACCCACCAATGGACAG
CAGGCTACTAGCCCCAATGAAGGTGTCACAATTGATCTGAAGAATTTCCGTAAACCTAGT
GAAAAGACTTTCACTCAAAGAAGCCGCTTGTTTGTGGGTAATCTCCCATCGGATGTTACT
GAGGAAGAAATGCGTAAATTGTTTGAAAAATTTGGCAAGGCAGGAGAGATCTTTATCCAC
AAGGACAAAGGTTTTGGATTCATTAGACTGGAAACACGTACACTGGCAGAGATAGCCAAA
GCAGAGCTGGATAACCTGCCTCTACGTGGGAAGCAATTACGTGTGAGGTTTGCCTGCCAT
AGTGCTGCACTGTCTGTTAAGAACATCCCTCAGTTTGTGTCAAATGAGCTGCTTGAGGAG
GCCTTCTCCATGTTTGGTCAAGTTGAGAGGGCAGTAGTAATTGTTGATGACCGTGGACGG
TCCACCGGAAAAGGAATTGTGGAGTTTGCATCAAAGCCATCTGCGCGGAAAGCTCTAGAC
CGCTGTAAAGACGGTGCCTATCTTCTCACTGCGTTTCCTCGTCCTATAACCGTAGAGCCC
ATGGACCAACTTGATGACGAGGAGGGTTTGCCTGACAAACTGCTAGTCAAGAATCAGATG
TGTCACAAGGAGAGAGAGCAACCTCCTCGCTTTGCTCAGCCTGGTTCTTTTGAATACGAA
TATGCAATGCGCTGGAAAGCCCTTATTGATATGGAGAAACAACAGCAACAGCAAGTGGAT
CGGAACATAAAGGAAGCCCAAGAGAAGATGGAAATCGAAATGGAAGCAGCAAGACATGAG
CACCAGGTTATGCTTATGCGTCAAGATCTAATGAGACGTCAGGAAGAGCTCCACCGAATG
GAAGAACTACACAATCAAGAAATCCAGAAACGCAAGCAAATCGAGATCCGACATGAGGAG
GAGCGCAGGAGGCGTGAGGAAGAGACCCGCCGACAACAAGAGGAGATGTTGAGACGTCAG
CAAGAAGGTTTCAAAAATAGCTACCCTGATGCTAGGGAAGCTGACTTACGAATGGCACAG
ATGGCAATGCCAGGCGCAATGGGATTGAATAATAGGGCTTCGCTGGGCAACAGTAATGTT
GCAAGTGGTGCTGCGGCTACCCCTGGACCTGGTGCAATGATGCCTGAAGCAGCAGCAATT
GGAATGACATCCCCTCCAAACGACCGTTTTGGGCAAGCCCCCAACATAGAAGGTCTTGGT
GGAGCCAACCCACCCGCCTTTCCCCGGGGTGCTCCAGGGGCTGATTTTGGTCCCAACAAA
CGTCGGCGATATTAA

Protein sequence predicted based on nucleotide sequence

MQGNRGYRMEQQNHTPRRQQQQQQHHQQQQPETTSPTNGQQATSPNEGVTIDLKNFRKPS
EKTFTQRSRLFVGNLPSDVTEEEMRKLFEKFGKAGEIFIHKDKGFGFIRLETRTLAEIAK
AELDNLPLRGKQLRVRFACHSAALSVKNIPQFVSNELLEEAFSMFGQVERAVVIVDDRGR
STGKGIVEFASKPSARKALDRCKDGAYLLTAFPRPITVEPMDQLDDEEGLPDKLLVKNQM
CHKEREQPPRFAQPGSFEYEYAMRWKALIDMEKQQQQQVDRNIKEAQEKMEIEMEAARHE
HQVMLMRQDLMRRQEELHRMEELHNQEIQKRKQIEIRHEEERRRREEETRRQQEEMLRRQ
QEGFKNSYPDAREADLRMAQMAMPGAMGLNNRASLGNSNVASGAAATPGPGAMMPEAAAI
GMTSPPNDRFGQAPNIEGLGGANPPAFPRGAPGADFGPNKRRRY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable