sars (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAGCTGAATTAACATTCATGTTCTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGTTTTAGATTTAGATCTTTTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714935
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1530nts
Open reading frame: 1 to 1530

ATGGTTTTAGATTTAGATCTTTTCCGGGAGGACAAGGGAGGAAACCCGGAGCTCATCAGA
GAGACTCAGAGAAAAAGATTCAAGGACCCGGGACTGGTAGACGCATTGCTGAACTCAGAC
ACAGCCTGGAGAAAGTGCCGGTTTCAGGCAGACAATCTTAATAAACAGAAAAATCTTTGC
AGCAAAATCATCGGGGAGAAAATGAAGAAAAAGGAACCTTTGGGAGACAGTGATGTGCTT
CCTGAAAATATCCAGCTTGACCAGCTGACAGCTGAAATTCTCAGTGCTTTGTCAGTTACA
CAGATAAAGAGACTGCGGGTTTTAATAGATGAAGCCATAACCGCAACTGACTCAGAACGT
ATCAAGCTGGAAGGGGAGAGATTTGAAAGTTTACGTGAGATTGGGAACCTGCTTCACCCA
ACAGTGCCTATCAGCAATGATGAGGACAATGATAACAAGGTGGAACGCACTTGGGGAGAC
TGTGAAGTTCAAAAGAGATACTCGCATGTGGACCTTGTGGTTATGGTGGATGGCTTTGAG
GGTGAAAAAGGAGCTGTAGTAGCTGGGAGCAGAGGATATTTCTTAAAGGGTCCACTGGTG
TTTCTAGAGCAGGCCCTAATACAGCTTGCTTTGCATACTCTGGCAGAAAAGGGATACACC
CCGATATATACCCCATTCTTCATGAGGAAAGAGGTCATGCAGGAGGTGGCCCAGCTCAGT
CAGTTTGATGAAGAACTGTACAAGGTGATAGGTAAAAGCAGTGAAAAATCTGATGACAAC
TCTATAGATGAGAAGTACCTGATAGCCACTTCAGAACAGCCAATAGCTGCATTACATCGG
GATGAGTGGCTGAAGCCTGAAGATTTACCTATACGATATGCTGGCCTATCAACCTGTTTC
CGGCAGGAAGTGGGGTCCCATGGAAGGGACACACGAGGCATATTTAGAGTACACCAGTTT
GAGAAGATTGAGCAGTTTGTTTATGCCTCACCTCATGATAACAAGTCCTGGGAAATGTTA
GAAGAAATGATTATGACAGCCGAATCATTTTACCAGATGCTGGGCATCCCATATCGTATT
GTGAATATTGTTTCAGGCTCATTGAATCATGCTGCCAGTAAGAAGCTGGATTTAGAGGCC
TGGTTTCCTGGCTCCAGTGCATTCAGAGAGCTGGTCTCTTGTTCTAACTGCACTGACTAC
CAAGCCCGTCGGCTGCGGATCCGCTATGGACAGACAAAGAAGATGATGGATAAGGTAGAG
TTCGTACACATGTTAAATGCTACTATGTGTGCCACAACCCGTGTAATTTGCGCAATCCTA
GAGAACTATCAGACAGAGGAAGGCATAGTTATACCAGAGAAACTCAGGGACTTTATGCCT
CCAGGTCTAAAGGATATTATAAAGTTTGTAAAACCAGCTCCAATTGACCAGGAACTCTCC
AAAAAGCAGAAGAAGCAACAGGAAAGAGGAAAGAAGACAGAAAATTGTGGTTTAGATTCT
CAAATGGAGAACATGAATGTTAATTCAGCTTAA

Protein sequence predicted based on nucleotide sequence

MVLDLDLFREDKGGNPELIRETQRKRFKDPGLVDALLNSDTAWRKCRFQADNLNKQKNLC
SKIIGEKMKKKEPLGDSDVLPENIQLDQLTAEILSALSVTQIKRLRVLIDEAITATDSER
IKLEGERFESLREIGNLLHPTVPISNDEDNDNKVERTWGDCEVQKRYSHVDLVVMVDGFE
GEKGAVVAGSRGYFLKGPLVFLEQALIQLALHTLAEKGYTPIYTPFFMRKEVMQEVAQLS
QFDEELYKVIGKSSEKSDDNSIDEKYLIATSEQPIAALHRDEWLKPEDLPIRYAGLSTCF
RQEVGSHGRDTRGIFRVHQFEKIEQFVYASPHDNKSWEMLEEMIMTAESFYQMLGIPYRI
VNIVSGSLNHAASKKLDLEAWFPGSSAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKVE
FVHMLNATMCATTRVICAILENYQTEEGIVIPEKLRDFMPPGLKDIIKFVKPAPIDQELS
KKQKKQQERGKKTENCGLDSQMENMNVNSA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable