gcm1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATAGGATTGGAGAAAAATGATTGGG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCTAAGTCTTCCCCTAAATTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00715006
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1182nts
Open reading frame: 1 to 1182

ATGCTAAGTCTTCCCCTAAATTCTGCTATGGAACCAGAACCCACAAGCTCAGAAAGCAAA
GGCATTAATTGGGACATAAATGATATGAAGCTTCCGCAGGATCCAGAACAAACTGATTGG
TTCCAGGAGTGGCCTGATTCATATGTAAAGCACATTTACAGCTCCTCCAGTAGAAATGCA
CAGAGACATTTAAGTGGGTGGGCAATGCGGAACACCAATAATCACAACTCACGTATTCTG
AAGAAGTCCTGCCTTGGTGTTCTTGTGTGTAGCAGTGACTGTACAGCCCCGGATGGAAGA
AAGGTATATCTGAGGCCTGCTATATGTGACAAGGCACGGCAAAAGCAGCAAAGTAAAAAT
TGCCCAAATTGCAGTGGACCTTTGAAATTAATTTCTTGCCGGGGTCACGGTGGATTTCCA
GTTACCAATTTCTGGAGGCATGAAGGGCCATACATATATTTTCAGACAAAGGGAGTTCAC
GACCACCCAAAGCCAGAAACAAAACTTGAGTCAGAAAGTAGAAAGGCTGTGCATAAGAAG
CGGACTGCAATTGTAACCACCACCCTTGGACTAAAAAGGAGCAGGAATGATGAGGCTCTC
GCAGGTGAAAAATCTGATCAGGAGAACCAACCTGGCCGGCAGGACTCTCAAGCTCTGCTA
TATGATTTCAATGACATGGATATGGATCACTCGGAAACAGAGAACATGCTCAACAACTGC
CTTACCAACTATCATTTTGGGAAGGATTCAAATCAGGGGGAAAATATAAGTGACCCAGTA
TGGAGCAAAAATCTGAGCAGAGACACCCAGAATGACAGCAATCAATGCATTGGAGGCGAG
TTTATAAGTTCGGCTATGGAATTTGATTTTTACAGTGAAGATCAACAAATGTGGAGCAAA
GTTTTCTTTTCCAAAAATCCACATACACAACCACTAGAATACCAATGTTGGGAAAAATCA
CCTTTGCATAGCTGCCATGCCTTAAATAACCAACACATTTCCAATGCGGAACCTTCAATA
ATGGATGGCCTTCTAAACTTTAAATCCAACGTGGATGATGTACATGAAGCAATGTTGCAA
GTGAACCATAACAATAGTGTATCTTCATCAGCAGTCTACCGCCTCTGCCAGGAGGACTCC
TATATACATAGATACAATCCCAATCATTTTTCTCCAATCCTATAA

Protein sequence predicted based on nucleotide sequence

MLSLPLNSAMEPEPTSSESKGINWDINDMKLPQDPEQTDWFQEWPDSYVKHIYSSSSRNA
QRHLSGWAMRNTNNHNSRILKKSCLGVLVCSSDCTAPDGRKVYLRPAICDKARQKQQSKN
CPNCSGPLKLISCRGHGGFPVTNFWRHEGPYIYFQTKGVHDHPKPETKLESESRKAVHKK
RTAIVTTTLGLKRSRNDEALAGEKSDQENQPGRQDSQALLYDFNDMDMDHSETENMLNNC
LTNYHFGKDSNQGENISDPVWSKNLSRDTQNDSNQCIGGEFISSAMEFDFYSEDQQMWSK
VFFSKNPHTQPLEYQCWEKSPLHSCHALNNQHISNAEPSIMDGLLNFKSNVDDVHEAMLQ
VNHNNSVSSSAVYRLCQEDSYIHRYNPNHFSPIL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable