bre (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAGTTTTCCATTGGCAAAAGCAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCTCCAGAAGTGACGTT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00715609
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1152nts
Open reading frame: 1 to 1152

ATGTCTCCAGAAGTGACGTTAAACCGGATATCACCTGCCCTCTCCCCATTTATCTCTAGC
GTCGTCAGAAATGGAAAAGTAGGTTTGGACTCCACAAACTGCTTGAGGATTACAGACCTT
AAGTCAGGATGTACATCCCTCACGCCAGGGCCAAGCTGTGATCGCTTTAAACTCCACATT
CCATATGCTGGGGAAACACTAAAGTGGGATATTATATTCAATGCTAGCTACCCCGAGCTG
CCCCCTGACTTCATATTTGGGGAAGATGCAGAATTTCTACCCGATCCGTCTGCTCTGCAT
AACCTGTCAGAATGGAACCCGTCAGACCCCGAGTGCCTGCTTTTGGTAGTGAAGGAACTG
GTTCAGCAGTACCACCAGTACCAGTGCAGCCGGCTCAGTGAGAGCTCACGCCTCATGTTT
GAGTATCAGACGCTGCAGGAGGAGCCGCAGTATGGATTAAATATGGAAATCTATGCGGGA
AAAAAGAACAACTGGACTGGGGAATTCTCTGCTCGCTTCCTGCTCAAGATGCCAGTTGAT
TTCAGCAATATTCCCATCTATCTTCTAAAGGACTCTAATGAAGACCCAGGGGAGGATGTT
GCTCTTCTGTCAGTCAGTTTTGAGGACGCAGAAGCCACTCAGGTTTTCCCCAAGCTGTTC
CTGTCTCCGCGAATTGAGCATGCCCTTGGTGGATCCTCAGCGCTTCATATCCCTGTGTTT
CCAAGTGGGAGCTGTCTTATTGATTATGTCCCTCAGGTCTGCCAGTTGCTAACTAACAAG
GTGCAGTATGTGATCCAAGGGTACCACAAGAGACGGGAATATATTGCTGCTTTTCTCAGC
CACTTTGGGACTGGCGTGGTAGAATATGATGCTGAAGGCTTCACCAAGCTGACCCTGTTG
CTGAGTTGGAAAGACTTCTGTTTCCTGGTGCACACAGTTGATCTGCCGCTGTACTTTCCA
CGAGACCAGCCAACTCTAACATTTCAGTCCGTGTATCACTTCACCAACAGCGGGCAGCTC
TATTCCCAGGCCCAGAAGAACTATCCATACAGCCCCCGCTGGGATGGGAACGAAATGGCC
AAGAGGGCAAAGGCCTATTTCCGGAGCTTCGTACCTCAGTTCCAGGAAGCTGCTTTTGCC
AATGGAAAACTCTAA

Protein sequence predicted based on nucleotide sequence

MSPEVTLNRISPALSPFISSVVRNGKVGLDSTNCLRITDLKSGCTSLTPGPSCDRFKLHI
PYAGETLKWDIIFNASYPELPPDFIFGEDAEFLPDPSALHNLSEWNPSDPECLLLVVKEL
VQQYHQYQCSRLSESSRLMFEYQTLQEEPQYGLNMEIYAGKKNNWTGEFSARFLLKMPVD
FSNIPIYLLKDSNEDPGEDVALLSVSFEDAEATQVFPKLFLSPRIEHALGGSSALHIPVF
PSGSCLIDYVPQVCQLLTNKVQYVIQGYHKRREYIAAFLSHFGTGVVEYDAEGFTKLTLL
LSWKDFCFLVHTVDLPLYFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYSPRWDGNEMA
KRAKAYFRSFVPQFQEAAFANGKL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable