gnl3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  gnl3
Gene Name:  guanine nucleotide binding protein-like 3 (nucleolar)
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATACAAAGTCGGTGTTGAAATCGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAACGTCCGAAGCTAAGGAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00715970
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1626nts
Open reading frame: 1 to 1626

ATGAAACGTCCGAAGCTAAGGAAATCCAGCAAACGCCTGAGCTGCCACAAACGCTACAAA
ATCCAGAAAAAGGTCCGGGAGCACAAGCGCAAGGTGAAGAAGGAGGCCAAGAAGAGCGGC
CCCAGGAAACACAAGAAAGACATCACTGTCCCTAACGCTGCCCCATTCAAAGCTCAGATC
CTGCAGGAGGCGCAACTCCGACGCCAGCAGCAAGAGGAATTGAAGCAAAAGCAGAAATTA
GATCGACAGAAGGAAGTGGCAAAGAAACGAAAGTTGGAGGAGAAGAAAAATAACAATTCT
GAAAAGAAAGAAAAAAGGGATAAAAAGAGAAAAGAAACTAAAACTCCTGACGATCCGGAC
GTGGTTTTGTGTCGGCAGGTGAACAAGGTGCTGGAGCAGAGCGATGTTATTGTGGAGGTT
CTGGATGCCAGGGACCCATTGGGCAGTCGATGTTCTCAGGCGGAGGAAGTCGTCCTTAAA
TCCCCCAATAAGAGACTTCTGCTCCTTCTCAATAAATCAGATCTTGTGCCCAGGGAGATG
GTAGAGAAGTGGCTGCAGGTTCTGAGCGCTGAGCTTCCCACCGTGCCGTTCAGATGTGTT
GCACAAATACAGGACAAGAGTGAGAAGAAGAAGAAGAAGAAGGTTCCCGTCAGCGCTGAT
CTGGTCACAGACCCCAAATGTCCCGGGGGGCAGGTCTTACTGAAGATCCTTCACAGCCTG
TGCCCCTCCCACAATGAGGCAATTAAAGTGGGTGTGATTGGATTTGCCAACGTGGGGAAG
AGCAGTGTCATCAACAGCCTGAAACAGTCTCACGTGTGTAACGTCGGCCCCAGCAAAGGC
ACCACAAAGTTCCTGCAGGAGGTGCGACTGGACCCCCAGATTAGACTGCTAGACAGCCCT
GCCCTGTTGGTGTCGCCCCACAATCCGCCAGTTGCGCTCATGTTGAGAAGTGCGTCTGAG
AGTAAAGTGGACGTGTTGGCTGCGGTGGAAGCCATCTTGAAACACTGCTCCAAACAGGAG
CTGATGTTGCACTACACCATTGCTGACTATAGGAACAGCCTGGAATGTCTCACGTTACTC
GCGCACAGACGGGGGATGCTGAAGAAAGGGGGCGTGCCTGACACAGAGGGGGCCGGACGA
CTGCTGTTTAATGATTGGATGGGAGCAAGAATGAAATATTACTGCCGGCCCCCCGACTCT
CCTGTCTGTCAGCCCCACATCAGCAGAGAGGTTGTGGCAGCGATGAGCAGCGGCATCTGT
AGCGAGGAGCTGGCCATGGACAACGCTTCTACCCTGAAAGCCCTAAAATGCCCCAACTCT
GCCAACATAGTGGTCTTTGCCTCCGTTGGACCTACCAGCGCCATCATGGATGAGACCCAA
TTGGTGGATCCAGAACCAGAGCTGATTGAAGAAGAGTTAGAAGAAAATGATGGAGAGGAG
GAGGAGGATATGGAGGAGGAAGAAGATGATGATGAAGGCAGTGAGGAGGAAGAGGACCTG
GAAGCCAAGAGTCTGACACAGGAAGTGACATCTGCAAAAGAAAAGGATTCAAAATCAGCC
GGTCCGGCCGTGAGCTTTGATCAGGCTGCAGATGATGACACTTACGATTTCAACACCGAC
TTTGTATAA

Protein sequence predicted based on nucleotide sequence

MKRPKLRKSSKRLSCHKRYKIQKKVREHKRKVKKEAKKSGPRKHKKDITVPNAAPFKAQI
LQEAQLRRQQQEELKQKQKLDRQKEVAKKRKLEEKKNNNSEKKEKRDKKRKETKTPDDPD
VVLCRQVNKVLEQSDVIVEVLDARDPLGSRCSQAEEVVLKSPNKRLLLLLNKSDLVPREM
VEKWLQVLSAELPTVPFRCVAQIQDKSEKKKKKKVPVSADLVTDPKCPGGQVLLKILHSL
CPSHNEAIKVGVIGFANVGKSSVINSLKQSHVCNVGPSKGTTKFLQEVRLDPQIRLLDSP
ALLVSPHNPPVALMLRSASESKVDVLAAVEAILKHCSKQELMLHYTIADYRNSLECLTLL
AHRRGMLKKGGVPDTEGAGRLLFNDWMGARMKYYCRPPDSPVCQPHISREVVAAMSSGIC
SEELAMDNASTLKALKCPNSANIVVFASVGPTSAIMDETQLVDPEPELIEEELEENDGEE
EEDMEEEEDDDEGSEEEEDLEAKSLTQEVTSAKEKDSKSAGPAVSFDQAADDDTYDFNTD
FV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable