dus3l (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATTTATAAGAGTTGGCTTTGTGCTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCAGAAAGTGAAGGCTCGAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716130
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1920nts
Open reading frame: 1 to 1920

ATGGCAGAAAGTGAAGGCTCGAATACTGAAAATGGCAAAGTGGGTGCCGTAAAATCTGAA
AATCTGGACCGTGGTGTAGCAGCCATTAAAAACCAATTTCTGACCACCAAAGAGAAGTTT
CATGCTTTCATCGATGCTGATGGAAAGGACGTTACAGAAAAGGAAACTTGTTCAGAGCTG
TCTCTTAATGATGCAGAGAACACGACCCGTACCGAGAATGCAGCAGAACCTGAAGCAAAA
CGAATTAAGCTTGATGATGGGAGTAGTGAAGGCCAGGACCAACCCCCCAAGACTGCAGAG
AACAAGCAAGAAAAGAAAAGAGCCAGAGGACAGAACAAAAGTCGACCTCACATGAAACAT
TCCCAGTTTGAAGAAAATAAACTGTGTCCATCAGTTACTCAGGAATGTGCCAGTAAATGT
TTTTTTGGAGACAAATGCAAGTTTTCGCACGATGTTGCCAAATATGTATCACAAAAGCCA
GAGGATATTCGCCCAAACTGTCACCTGTATGAGACTTTTGGGAAGTGCATTTATGGAGTC
ACATGTCGGTTCGCCAAATCACACCTGGGGGAGGATTTTAAGAACATAATTAATGAAGAG
CTTATGAAGGAGTGGGAAGGCAAAGTATTGGTGAAAAACAGCCTAGACAAAAGCTTAAAG
GAACAGTTACGAAAAAAGAAAGTTGTGTTTGAGAAATCCGATAAATATTTAAAGCTTTGC
TTCAAATCTGGAGACAGTTCAAAAATGAAGAACCCAGTAGTTAAGGAAGACAGCGCTGTG
CAAGTTACACAAAAGGACAGTCCAATTACGACAGTGGGAGCTGTGACAGATGAAGATGTT
ATAAAACTCAGGCCTTGTGAGAAAAAAACTATTGATTTCAGGAACAAGCTTTATCTAGCA
CCATTGACTACGTGTGGCAATCTGCCTTTCAGAAGAATATGTAAGCGATTAGGAGCAGAC
ATTACGTGTGGAGAAATGGCCATGTGCACCAACCTGCTTCAGGGACAGCCTTCTGAGTGG
GCTTTGTTAAAGCGCCATCATTCAGAGGATATTTTTGGAGTGCAGCTGGAGGGAGCGTTT
CCTGACACAATGACTAAGTGCGCTGAGCTGCTCAACAGAACCATCGATGTAGACTTTGTG
GATATCAATGTTGGATGCCCCATAGATCTGGTGTACAAAAAGGGTGGTGGTTGTGGCTTA
ATGAACAGGACTAATAAATTTGAACAGATCGTGAAAGGAATGAATTCTGTCCTTGATGTT
CCATTGACCGTAAAAATCAGGACTGGGGTGCAGGAAAAAGTCAACATTGCCCACAAACTT
ATTCCGAATCTCAGAGACTGGGGAGTCTCATTGGTTACACTTCATGGAAGATCCAGAGAA
CAACGATACACAAAACTAGCAAACTGGGAATACATAGATCAGTGTGCGAAAATAGCAAGT
CCTGTCCCCTTGTTTGGCAATGGAGACATCATATCCTATGAGGATGCAAATCGAGCTTTG
CAGACTGGAGTTGCAGGAGTGATGTTGGCAAGAGGAGCGTTATTCAAGCCATGGCTGTTT
ACTGAAATTAAAGAGCAGCGGCACTGGGATATTTCCTCAACGGAACGATTTGACATCTTA
AAGGACTTTACAAATTACGGCCTGGAACACTGGGGTTCAGATTGCCAGGGGGTTGAGAAA
ACAAGAAAATTCATGCTGGAGTGGCTATCCTTTTTATGCAGGTATATTCCCGTTGGTTTA
CTGGAGCATGTACCTCAGAAGATAAATGAAAGGCCTCCATACTACATGGGGAGAGACTAC
TTGGAGACTTTAATGGCAAGTCAGAACGTAACGGACTGGATAAAAATAAGTGAGATGCTT
TTGGGACCTGTTCCTCCGAACTTCACCTTTCTGCCAAAGCACAAAGCCAACTCTTATAAA
TAA

Protein sequence predicted based on nucleotide sequence

MAESEGSNTENGKVGAVKSENLDRGVAAIKNQFLTTKEKFHAFIDADGKDVTEKETCSEL
SLNDAENTTRTENAAEPEAKRIKLDDGSSEGQDQPPKTAENKQEKKRARGQNKSRPHMKH
SQFEENKLCPSVTQECASKCFFGDKCKFSHDVAKYVSQKPEDIRPNCHLYETFGKCIYGV
TCRFAKSHLGEDFKNIINEELMKEWEGKVLVKNSLDKSLKEQLRKKKVVFEKSDKYLKLC
FKSGDSSKMKNPVVKEDSAVQVTQKDSPITTVGAVTDEDVIKLRPCEKKTIDFRNKLYLA
PLTTCGNLPFRRICKRLGADITCGEMAMCTNLLQGQPSEWALLKRHHSEDIFGVQLEGAF
PDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCGLMNRTNKFEQIVKGMNSVLDV
PLTVKIRTGVQEKVNIAHKLIPNLRDWGVSLVTLHGRSREQRYTKLANWEYIDQCAKIAS
PVPLFGNGDIISYEDANRALQTGVAGVMLARGALFKPWLFTEIKEQRHWDISSTERFDIL
KDFTNYGLEHWGSDCQGVEKTRKFMLEWLSFLCRYIPVGLLEHVPQKINERPPYYMGRDY
LETLMASQNVTDWIKISEMLLGPVPPNFTFLPKHKANSYK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable