irf3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATTCAAAGTCCATCATCTCGTG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGCTCACAGAAGCCAC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716140
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1398nts
Open reading frame: 1 to 1398

ATGGGCTCACAGAAGCCACGGATTATCCCATGGTTAATCAGCCAGATTGGTAACCCAGCA
TACCCGGGCCTGATTTGGATGAATGAAGAAAAGACCAGGTTTCGTATCCCTTGGAAACAT
GGATTGCGTCAGGACAGATGTGATGCTGATGTCAAAATATTTGAGGGCTGGGCTATTGTC
AGTGGTTCCTATGACCCCAGCAAGGATCAGCCTAACCCAGCGGTGTGGAAAAGGAATTTC
CGCTCGGCACTGAATCGCAAGGCTGGCATCAATGTGATGGTAGACAAGAGCTCGGAGTCT
GTTAACCCTCACAAGGTCTATGAGATCCAAAAAGGGATCAGCGTGGAGGGTGATGTTGGC
TCTGAAGAGACTTATATGAATGACAGCATTAGCCCTTCTTCTGATTTATTCTCAATAAAC
AGCCCCAGGAGCAACACAAGCAGCAATATGAATGCAACCCTATCAGAAGGCATAATGCAG
CTACAGCTCTCTCATACTGAAGAAGATCTATACTGTTCCCCTGAAGACAACCAAGCCATG
CCATATAGAGATCCATATAACTCTCAGGAAAGCTGGTTCTCTGGCATTTCCTCCCCGGAG
AGGGTTGGAGATGCTGATACCCCTGCATACAGTACAGCTCCTGTGCACATGGGGGCACAT
GCTGAAGCCTTACCGGCGGAGGTCGCCACAAATCAAGGAAGTCCACTACAGCAACATATC
ACTGATCATTTTTTCCAAGACAGGACATTAAGGACAGAATTCGAAGTGACTGTATATTAC
AGAGGAATCGAAGTTAGTAAAACTCTGGTAAAAAACCCACACGGCTTCCGCATTACATCG
AGAAAACACTCATCACCAGGCAGCTACTTGGACGATGTGGTTCTGCCATCGCCAACAATG
ATTGCCGATCAGGCTGTTGTGGGTGAAATACACAAACTGTTGCGGAACTTAGAAGAAGGG
GTCTTGGTCGAGGTGAGAGGAGGCTCCATCTGTGGGAAGAGGCAAGGAAAGTGCAGGGCT
TTTTGGAGCATGACCGAGATGCCTGAAATCAGTCAACCCAACCAGATTGACAAAAACGAC
TACTGCATTCTGCACACACTGCAGCAGTTTGTAGCAGAACTGACTGAATTCATCGAACGC
ACCAGGAAGTCCTCCCCTCAGTACCACATTTGGATGTGCCTTGGAGAACTGTGGCCTGAT
GTTCGGCCATGGAATAAAAAATTCATCATGGTGCAGATTGTACCTGTGTCAATGAAGTTG
CTTCATGACATGAGTTACAGTACGGGAGCCTCTTCTCTCCATAGCAGTGAGATTAACCTG
GAAATCTCAGACTCTCTCTCCAGTACAAATGACGTGATGGCTGTTCTACGAGAACTCCAC
GAGATGATGGACTTTGAATAG

Protein sequence predicted based on nucleotide sequence

MGSQKPRIIPWLISQIGNPAYPGLIWMNEEKTRFRIPWKHGLRQDRCDADVKIFEGWAIV
SGSYDPSKDQPNPAVWKRNFRSALNRKAGINVMVDKSSESVNPHKVYEIQKGISVEGDVG
SEETYMNDSISPSSDLFSINSPRSNTSSNMNATLSEGIMQLQLSHTEEDLYCSPEDNQAM
PYRDPYNSQESWFSGISSPERVGDADTPAYSTAPVHMGAHAEALPAEVATNQGSPLQQHI
TDHFFQDRTLRTEFEVTVYYRGIEVSKTLVKNPHGFRITSRKHSSPGSYLDDVVLPSPTM
IADQAVVGEIHKLLRNLEEGVLVEVRGGSICGKRQGKCRAFWSMTEMPEISQPNQIDKND
YCILHTLQQFVAELTEFIERTRKSSPQYHIWMCLGELWPDVRPWNKKFIMVQIVPVSMKL
LHDMSYSTGASSLHSSEINLEISDSLSSTNDVMAVLRELHEMMDFE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable