acad9 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGGTTCTGTCCAGCGGGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTTCGGGTTACGGCGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716386
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1866nts
Open reading frame: 1 to 1866

ATGTTCGGGTTACGGCGGGTGTGCGGCCGGGCGGCGAGTTTAACCCGAGTGTGGGCGGGT
CCGGCGGGGCCTGAGCTTTTCCCGAGGAGCTTTACTTGTTCTCCCCACAGACTCGCCTAC
GCCAAAGAGCTGTTCCTCGGCTCCATCCGGAAGGACGAGGTTTTCCCCTACCCTGAGATC
AGCAAAGAGGAGCTGGAAGAGATCAACCAGTTTGTGGCCCCCGTGGAGAAGTTCTTCAAT
GAGGAAGTGGACTCCAAACTGATTGATCAGACTGCCAAGATTCCTCCTGAAACTCTGGAT
GGATTAAAGAACCTGGGTCTGTTTGGCATGCAAATCCCAGAAGAGTATGGGGGGCTGGGA
TTATCCAATACGATGTATGCACGGCTGGGAGAAATCATCTCTCTGGATGGAGCAATTGCT
GTTACCCTGGCCGCTCACCAGGCCATAGGACTGAAGGGGATTCTGATTGCTGGAAACGAT
GAGCAAAAGGCCAAATATCTACCGAAACTGGCATCTGGGGAGCACATTGCAGCCTTCTGC
CTCACTGAGCCGGGCAGTGGCAGTGATGCTGCATCCATCCAATCCAGGGCAACTCTGACC
CCAGACGGGAAGCACTTTCTCTTGAATGGATCCAAGATCTGGATCTCTAACGGTGGATTG
GCCGACATATTTACCGTCTTCGCCAGAACCGAGGTGGTGGATAAAGACGGTGTAACTAAA
GATAAGATTAGCGCTTTCATTGTGGAACGGGCGTTTGGCGGCGTGACCCACGGGAAACCA
GAGGATAAACTAGGGATCCGGGGATCTAACACGTGTGAGCTCCATTTTGAAAACACAAAG
GTGCCTGTGGAAAATGTGATTGGGGAGGTTGGAGGGGGCTTTAAGGTGGCAATGAATATT
CTAAACAGCGGCAGATTCAGCATGGGCAGCGCCTCGGCCGGCATAATTAAGAAGCTCATA
GAGATGACGGCAGAGTACGCCTGCACCCGGAAGCAGTTCAACAAGAAGCTCAGCGACTTT
CAACTCATTCAGGAGAAATTTGCTCTCATGGCACAGAAGGCGTTTGTGATGGAAAGTATG
GCTTACCTGACTGCCGGCATGATGGACCGGCCCGGGCTTCCTGATTGCTCAGTTGAGGCA
GCTATGGTTAAGGTGTTCAGCTCGGAGGGGGCGTGGCTGTGCGTGAGCGAGGCTCTGCAG
ATCCTGGGGGGATTGGGCTACATGAAGGATTATCCATACGAGCGATATCTCAGGGACAGC
CGGATTCTCCTCATATTTGAGGGGACGAATGAGATCCTGCGAATGTACATTGCACTCACC
GGGATGCAGCACGCCGGCAAAATCCTCACTGGCAAAATCAAAGAAATGAAGAAAGGGAAC
GTTGGCGTTGCTATGGAATTGTTCTTTAAGAAACTGAGAGACAGCATGGGAAGGAACGTT
AATTTGGGATTGACTGGGAATGAGGGTGTCGTTCATCCAAGCCTGGAGGAGAGTGCCAAG
AAGCTGGAGGAGAATGTCTATTATTTTGGCACCACAGTAGAGAGTTTGCTGTACCGATTT
GGCAAGACTATTGTGGAGGAGCAGTTGGCGCTGAAGAGAGTGGCTGATGTGATTATAAAT
CTATACGCCATGACGGCCGTCATTTCCAGAGCGAGTCGATCAATCAGTGTGGGGCTGCCC
AATCACGACCACGATGTGCTGCTGGCCAACATGTTCTGCTCCGAGGTTTATTTCAAGAAC
AACTACACCCTGGCACAACTGGGCAAACATTCTCCAGAGAACCAGGATGACAGTATCAGG
AAGATAGCGGGGCACATTCTGGAGAAGAGGGGGTACACCTGCTCCCACCCGCTGGACAGA
ACCTTCTGA

Protein sequence predicted based on nucleotide sequence

MFGLRRVCGRAASLTRVWAGPAGPELFPRSFTCSPHRLAYAKELFLGSIRKDEVFPYPEI
SKEELEEINQFVAPVEKFFNEEVDSKLIDQTAKIPPETLDGLKNLGLFGMQIPEEYGGLG
LSNTMYARLGEIISLDGAIAVTLAAHQAIGLKGILIAGNDEQKAKYLPKLASGEHIAAFC
LTEPGSGSDAASIQSRATLTPDGKHFLLNGSKIWISNGGLADIFTVFARTEVVDKDGVTK
DKISAFIVERAFGGVTHGKPEDKLGIRGSNTCELHFENTKVPVENVIGEVGGGFKVAMNI
LNSGRFSMGSASAGIIKKLIEMTAEYACTRKQFNKKLSDFQLIQEKFALMAQKAFVMESM
AYLTAGMMDRPGLPDCSVEAAMVKVFSSEGAWLCVSEALQILGGLGYMKDYPYERYLRDS
RILLIFEGTNEILRMYIALTGMQHAGKILTGKIKEMKKGNVGVAMELFFKKLRDSMGRNV
NLGLTGNEGVVHPSLEESAKKLEENVYYFGTTVESLLYRFGKTIVEEQLALKRVADVIIN
LYAMTAVISRASRSISVGLPNHDHDVLLANMFCSEVYFKNNYTLAQLGKHSPENQDDSIR
KIAGHILEKRGYTCSHPLDRTF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable