rnf26 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAATGTAGACATTTAGGGTTTGCAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGGCGATCGTTATG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716478
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1320nts
Open reading frame: 1 to 1320

ATGCAGGCGATCGTTATGCTCCTCAATGGGCTTGGTTGGACCCTGGACATGTTGCTTCTG
TTGCTGGACTTAAATTACTTTCTGGTTTCCTCTGTTGTATCAGTGTTGTTTTTGACCATT
CGCTTCATCTTCAATCTTCCTTGGACCATCACCAATGGCATTCTTCAGTTGTGCGAATCT
TTATATGGATTTGTGTTTATGGTTGGTGATTCCTCCTGCAGCCTTGTACAGACAGTACTG
GATGTTTTGTGGGGATGCCTTGCTGGTTTAGACAGTTTGAAGTTAATATGGAACTTGCTG
TGCCATCTACTATTCCGCAGCAGAGAGCTGTTTCATCGAGGGCTGCTGAACATCACTGTC
TATGTACAGACTTTTCACCATAATTTTTGGGAAGCGTTAAGCATCACTGGAAGTTTGGCA
GCTTACTTGGTTAATAGCCTAGTAAACATATGTCTTATAGCCATCCAAAATGTTTTATCT
GCTGCAATGGCTCTCTGGCTTTCCATGGTCAATGTGATCTTCATGGGAGCAGAGCTTGTA
ATCATGTTGTTGTCTCAGTTTTCCAACAGTGCTGTGGCAGTGTCAATTCTCTTGTGGACT
CCTTGTCAGCTGATTTTAGATGGCTTTGCCTCACTTAGTAGAGGAGTAGGAATAATATTA
TATAAACACCTATATGCGATTCTTTTCTTTCTGCTTCTCACCATTATTTGCAGAATTATT
TTTAGACCTTCCCCTGCTGTCCGCCAGTTTCAGTTAAAAGTAATTCAGATGTACCGCATT
GCACTTGTGCTTGCCTGTTCACTTCTGCATTGTGAGTTTTTGAGAAGAGTCATTGCAAAA
AGTGTGCAGATAATAAGAATGTTTAGAATATATGGAACAGCTCTGGTCAGAGGATGGAAT
TTGAGAAGAACTAGAATACAAAATCCACCTGTTAGGCCTGCCACTGTGCAGAATCCAATA
CGAATAAGGGAGCCAAACCCAGTTCCCGCGAGGGAGCAGAATCCACCATTGAGACCTAGT
CAGAGCTCGGTTCCTAACAGTGCACAGAACACGCAACCTGCAACCCACATTCCTCAAGCC
AGTTCAGCATATAAAAAAAGAGGGGAGCCCTCCCAAGATCCATGGAAACTTCTAAAGCAG
CAGGAAGAGAGCAAGAAATGTGTTATTTGCCAAGATGAGAACAAAACTGTACTACTTTTG
CCTTGCCGCCATTTATGCCTCTGTGCCGCCTGCACACAGATTCTTCTGCAACAGCCTGTC
CATCAGCGCAACTGCCCTCTGTGTAGGCAGATGATTCTGCAAACCCTAAATGTCTACATT
TAA

Protein sequence predicted based on nucleotide sequence

MQAIVMLLNGLGWTLDMLLLLLDLNYFLVSSVVSVLFLTIRFIFNLPWTITNGILQLCES
LYGFVFMVGDSSCSLVQTVLDVLWGCLAGLDSLKLIWNLLCHLLFRSRELFHRGLLNITV
YVQTFHHNFWEALSITGSLAAYLVNSLVNICLIAIQNVLSAAMALWLSMVNVIFMGAELV
IMLLSQFSNSAVAVSILLWTPCQLILDGFASLSRGVGIILYKHLYAILFFLLLTIICRII
FRPSPAVRQFQLKVIQMYRIALVLACSLLHCEFLRRVIAKSVQIIRMFRIYGTALVRGWN
LRRTRIQNPPVRPATVQNPIRIREPNPVPAREQNPPLRPSQSSVPNSAQNTQPATHIPQA
SSAYKKRGEPSQDPWKLLKQQEESKKCVICQDENKTVLLLPCRHLCLCAACTQILLQQPV
HQRNCPLCRQMILQTLNVYI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable