tgfb1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  tgfb1
Gene Name:  transforming growth factor, beta 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGCTGCAGTTGCAAGACC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAGGTTCTGTGGATG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716731
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1146nts
Open reading frame: 1 to 1146

ATGGAGGTTCTGTGGATGCTGCTGGTGCTATTGGTCCTGCATCTTTCCAGTTTAGCTATG
TCCTTGTCTACCTGTAAGGCTGTGGATATGGAAGAAGTCAGGAAGAGGAGAATTGAAGCC
ATCAGGGGCCAAATTCTCAGCAAGCTAAAACTGGACAAAACACCAGATGTAGACAGCGAG
AAGATGACAGTGCCCAGTGAAGCCATCTTCCTTTACAATAGCACCTTGGAGGTGATCAGA
GAAAAAGCAACCAGAGAAGAAGAACATGTAGGGCATGATCAGAACATACAGGACTATTAT
GCAAAACAAGTTTACCGATTTGAAAGCATAACCGAACTGGAAGACCATGAATTTAAGTTT
AAATTTAATGCTTCGCATGTGAGGGAGAATGTAGGTATGAACTCTCTTCTGCACCATGCT
GAGCTGCGCATGTACAAGAAGCAGACAGATAAAAACATGGATCAAAGGATGGAACTATTC
TGGAAATACCAGGAAAATGGGACCACTCACTCAAGATACCTGGAGAGCAAGTACATTACT
CCAGTAACAGATGATGAGTGGATGTCATTTGATGTTACAAAGACTGTGAATGAATGGCTG
AAACGTGCAGAGGAAAATGAACAATTCGGGTTGCAGCCTGCCTGCAAATGTCCCACCCCA
CAGGCCAAAGATATAGACATAGAAGGATTTCCTGCTTTACGTGGAGACTTGGCGAGTTTG
AGTTCCAAAGAAAACACGAAACCCTACCTGATGATCACTTCGATGCCAGCTGAGCGTATT
GACACAGTTACAAGCTCACGGAAAAAGCGAGGGGTTGGACAGGAGTATTGCTTTGGGAAC
AATGGGCCAAACTGCTGTGTGAAACCTCTTTACATAAATTTTCGGAAGGATCTAGGCTGG
AAGTGGATCCATGAGCCTAAGGGATATGAAGCAAATTATTGTTTAGGAAATTGTCCTTAC
ATCTGGAGCATGGATACTCAGTACAGCAAGGTGCTATCACTTTATAATCAGAACAATCCC
GGTGCATCTATATCTCCCTGCTGTGTTCCTGATGTCTTGGAGCCACTGCCAATCATTTAT
TATGTTGGCCGCACTGCTAAAGTAGAGCAGCTCTCTAATATGGTGGTAAGGTCTTGCAAC
TGCAGCTGA

Protein sequence predicted based on nucleotide sequence

MEVLWMLLVLLVLHLSSLAMSLSTCKAVDMEEVRKRRIEAIRGQILSKLKLDKTPDVDSE
KMTVPSEAIFLYNSTLEVIREKATREEEHVGHDQNIQDYYAKQVYRFESITELEDHEFKF
KFNASHVRENVGMNSLLHHAELRMYKKQTDKNMDQRMELFWKYQENGTTHSRYLESKYIT
PVTDDEWMSFDVTKTVNEWLKRAEENEQFGLQPACKCPTPQAKDIDIEGFPALRGDLASL
SSKENTKPYLMITSMPAERIDTVTSSRKKRGVGQEYCFGNNGPNCCVKPLYINFRKDLGW
KWIHEPKGYEANYCLGNCPYIWSMDTQYSKVLSLYNQNNPGASISPCCVPDVLEPLPIIY
YVGRTAKVEQLSNMVVRSCNCS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable