ddx1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGCTTCGGAAAAGCTG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCGGCCTTCTCAGAAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716789
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2220nts
Open reading frame: 1 to 2220

ATGGCGGCCTTCTCAGAAATGGGGGTGATGCCCGAGATTGCCCAGGCTGTGGAGGAAATG
GATTGGCTTCTTCCTACAGATATCCAAGCAGAGTCCATACCCCTGATCCTCGGTGGTGGG
GATGTGCTGATGGCTGCAGAAACTGGAAGTGGAAAGACTGGGGCTTTCAGCATCCCTGTC
ATCCAGATTGTTTATGAAACACTTAAAGATCAACAAGAGGGCAAGAAAGGAAAAGCATCC
GTTAAGACTGGAAGTACAGTACTAAATAAATGGCAGATGAATCCATATGACAGAGGTTCA
GCTTTTGCGATTGGCTCAGATGGCCTTTGCTGCCAAAGCAGGGAAATAAAGGAGTGGCAT
GGCTGCAGGTCCACTCGAGGAGTAAATAAAGGAAAATACTATTATGAAGTATCCTGTCAT
GACCAGGGTTTATGCAGAGTTGGATGGTCTACCCTGTCAGCCTCCTTAGATCTTGGAACT
GATAAGTTTGGTTTTGGCTTTGGTGGAACCGGGAAAAAGTCGCACAACAAGCAATTTGAC
AATTATGGAGAGGAGTTTACAATGCACGACACCATTGGCTGTTACTTAGACATTGATAAC
AGCATAGTAAAGTTTTCCAAAAATGGTAAGGACCTTGGCCTTGCATTTCAGATCCCTTCT
CACATGAAGAACCAAGCTTTCTTTACATCATGTGTTCTAAAGAATGCTGAACTTAAATTC
AATTTTGGAGAGGAAGACTTCAAATTTCCCCCAAAGGATGGATTTGTGGCACTCTCAAAG
GCTCCAGATGGACATGTGGTGAAATCACAGAACACTGGAAGCGCGCAAGTGTCTCAAACA
AAGAGTTTACCGAATGCCCCCAAAGCACTTATCATCGAGCCCTCCCGTGAGCTGGCAGAG
CAGACTCTAAACAACGTCAAGCAATTTAAAAAATATGTTGACAACCCTAAACTGAGAGAA
TTGTTGATTATCGGTGGTGTTGCAGCAAAGGAACAGTTAACCATTCTCGAAAATGGTGTG
GATATAGTGGTGGGGACCCCTGGTAGGATAGATGATCTTATATCTACAGGGAAACTCAGC
CTGTCTCAAGTCCGTTTCCTGGTACTTGATGAAGCTGATGGTTTACTTTCACAAGGATAT
TCCGACTTCATCAACAGAATTTATGGGCAGATCCCTCAGATCACTTCAGATGGGAAAAGG
TTACAGGTGATTGTGTGCTCCGCCACGCTGCATTCCTTTGATGTAAAGAAGCTTTCTGAA
AAAATCATGCATTTCCCAACATGGGTGGACCTTAAAGGAGAGGATTCTGTGCCAGAAACT
GTGCATCATGTTGTTGTGCCGGTGAATCCCAAGAAAGACAAACAGTGGGAAAAACTTGCC
AAAAATCACATTAGGACTGATGGCGTGCATGATAAAGATAACACAAGGCCTGGTGGAAAT
TCCGCAGAGGTGTGGTCAGAAGCTATTAAAGTATTAAAAGGAGAATACATTGTGCGTGCC
ATCAAGGAGCATAAAATGGACCAAGCAATTATATTCTGCAGAACCAAATTAGACTGTGAT
AACATGGAGCAGTATTTCATCCAACAAGGAGGAGGACCTGATAAGAAAGGACATCAGTTT
TCATGTGTGTGTCTTCATAGTGACCGGAAGCCACCAGAAAGGAAACACAACCTGGAAAGA
TTTAAGAAATGTGAAGTTCGGTTCTTGATCTGCACTGATGTTGCTGCCAGAGGTATTGAT
ATTCGTGGAGTCCCATATGTTATTAATGTTACCCTCCCTGATGAGAAGCAGAACTACGTC
CATCGCATTGGTAGAGTGGGCCGAGCTGAAAGAATGGGTTTGGCCATATCTCTGGTGGCA
TCCGAAAAGGAGAAGGTTTGGTACCATGTGTGCAGCAGCCGTGGGAAGGGATGTTATAAC
ACCAGACTGAAGGAAGATGGGGGCTGTACTATATGGTACAATGAGATGCAGTTACTGTCT
GAGATTGAAGAGCATCTGACTTGCACTATATCCCAAGTAGAACCTGATATAAAAGTGCCC
TTGGATGATTTCGATGGAAAGGTGGTGTATGGGCAGAGAAGGGCTACCGGAGGTGGACTC
TATAAGGGTCATGTAGATATTTTGGCCCCAACTGTGCAAGAGTTGGCATCTCTAGAAAAA
GAGGCTCAGACGTCCTTCCTGCACCTGGGATACCTTTCTAATCAGCTTTTCCGAAGCTTC
TAA

Protein sequence predicted based on nucleotide sequence

MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPV
IQIVYETLKDQQEGKKGKASVKTGSTVLNKWQMNPYDRGSAFAIGSDGLCCQSREIKEWH
GCRSTRGVNKGKYYYEVSCHDQGLCRVGWSTLSASLDLGTDKFGFGFGGTGKKSHNKQFD
NYGEEFTMHDTIGCYLDIDNSIVKFSKNGKDLGLAFQIPSHMKNQAFFTSCVLKNAELKF
NFGEEDFKFPPKDGFVALSKAPDGHVVKSQNTGSAQVSQTKSLPNAPKALIIEPSRELAE
QTLNNVKQFKKYVDNPKLRELLIIGGVAAKEQLTILENGVDIVVGTPGRIDDLISTGKLS
LSQVRFLVLDEADGLLSQGYSDFINRIYGQIPQITSDGKRLQVIVCSATLHSFDVKKLSE
KIMHFPTWVDLKGEDSVPETVHHVVVPVNPKKDKQWEKLAKNHIRTDGVHDKDNTRPGGN
SAEVWSEAIKVLKGEYIVRAIKEHKMDQAIIFCRTKLDCDNMEQYFIQQGGGPDKKGHQF
SCVCLHSDRKPPERKHNLERFKKCEVRFLICTDVAARGIDIRGVPYVINVTLPDEKQNYV
HRIGRVGRAERMGLAISLVASEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLS
EIEEHLTCTISQVEPDIKVPLDDFDGKVVYGQRRATGGGLYKGHVDILAPTVQELASLEK
EAQTSFLHLGYLSNQLFRSF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable