Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00718739
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4314nts
Open reading frame: 1 to 4314
ATGAGTATGCTAAAGCCAAGTGGGCTTAAGGCCCCCACCAAGATCCTGAAGCCTGGAAGC
ACAGCTCTGAAGACACCTACGGCTGTTGTAGCTCCAGTAGAAAAAACCATATCCAGTGAA
AAAGCATCAAGCACTCCATCATCTGAGACTCAGGAGGAATTTGTGGATGACTTTCGAGTT
GGGGAGCGAGTTTGGGTGAATGGAAATAAGCCTGGATTTATCCAGTTTCTTGGAGAAACC
CAGTTTGCACCAGGCCAGTGGGCTGGAATTGTTTTAGATGAACCCATAGGCAAGAACGAT
GGTTCGGTGGCAGGAGTTCGGTATTTCCAGTGTGAACCTTTAAAGGGCATATTTACCCGA
CCTTCAAAGTTAACAAGGAAGGTGCAAGCAGAAGATGAAGCTAATGGCCTGCAGACAACG
CCCGCCTCCCGAGCTACTTCACCGCTGTGCACTTCTACGGCCAGCATGGTGTCTTCCTCC
CCCTCCACCCCTTCAAACATCCCTCAGAAACCATCACAGCCAGCAGCAAAGGAACCTTCA
GCTACGCCTCCGATCAGCAACCTTACAAAAACTGCCAGTGAATCTATCTCCAACCTTTCA
GAGGCTGGCTCAATCAAGAAAGGAGAAAGAGAGCTCAAAATCGGAGACAGAGTATTGGTT
GGTGGCACTAAGGCTGGTGTAGTCCGGTTTCTTGGGGAGACCGACTTTGCCAAGGGGGAG
TGGTGTGGCGTGGAGTTAGATGAGCCACTTGGGAAGAATGATGGCGCTGTTGCTGGAACA
AGGTATTTTCAGTGTCAACCCAAATATGGCTTGTTCGCTCCTGTCCACAAAGTTACCAAG
ATTGGCTTCCCTTCCACTACACCAGCCAAAGCCAAGGCCAACGCAGTGAGGCGAGTGATG
GCGACCACGTCCGCCAGCCTGAAGCGCAGCCCTTCTGCCTCTTCCCTCAGCTCCATGAGC
TCAGTGGCCTCCTCTGTGAGCAGCAGGCCCAGTCGGACAGGACTATTGACTGAAACCTCC
TCCCGTTACGCCAGGAAGATCTCCGGTACCACTGCCCTCCAGGAGGCCCTGAAGGAGAAG
CAGCAGCACATTGAGCAGCTGCTGGCGGAACGGGATCTGGAGAGGGCGGAGGTGGCCAAG
GCCACGAGCCACGTGGGGGAGATAGAGCAGGAGCTAGCTCTGGCCCGGGACGGACATGAC
CAGCATGTCCTGGAATTGGAAGCCAAAATGGACCAGCTGCGAACAATGGTGGAAGCTGCT
GACAGGGAGAAGGTGGAGCTTCTCAACCAGCTTGAAGAGGAGAAAAGGAAGGTTGAGGAC
CTTCAGTTCCGGGTTGAAGAAGAATCAATTACCAAAGGTGATCTTGAGCAAAAGAGCCAG
ATTTCTGAAGATCCTGAGAATACGCAGACCAAACTGGAGCATGCCCGCATTAAGGAGCTT
GAACAGAGCCTGCTCTTTGAAAAGACCAAAGCTGACAAACTCCAGAGGGAGTTAGAAGAC
ACTAGGGTGGCTACAGTTTCAGAAAAGTCACGTATAATGGAACTGGAGAAAGACCTAGCA
TTGAGAGTACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAATAAGCCTGCTGGG
GATGTGGACATGTCACTTTCCCTTTTGCAAGAGATAAGCTCTTTGCAAGAAAAGTTAGAA
GTCACCCGTACTGACCACCAGAGAGAAATAACTTCTCTGAAGGAGCATTTTGGAGCCCGG
GAAGAAACTCATCAGAAGGAGATAAAGGCTCTGTATACCGCCACGGAAAAGCTTTCCAAA
GAGAACGAGTCATTGAAAAGCAAGCTGGAGCATGCCAACAAAGAGAACTCAGATGTGATA
GCTCTATGGAAGTCCAAACTGGAGACTGCCATCGCATCCCACCAGCAGGCGATGGAAGAA
CTGAAGGTATCTTTCAGCAAAGGGCTTGGAACAGAGACGGCAGAATTTGCTGAACTAAAA
ACACAAATAGAGAAAATGAGACTAGATTACCAACACGAAATAGAAAATTTGCAGAATCAA
CAAGACTCTGAACGGGCTGCCCATGCTAAAGAGATGGAAGCCTTGAGGGCTAAACTGATG
AAAGTTATTAAAGAAAAGGAAAACAGTCTGGAAGCCATCAGGTCGAAACTGGACAAAGCA
GAAGACCAGCATCTCGTAGAAATGGAAGACACGTTAAACAAATTACAGGAAGCTGAAATA
AAGGTAAAGGAGCTAGAGGTACTGCAAGCCAAATGCAATGAACAAACCAAGGTTATTGAT
AATTTTACATCACAGCTCAAGGCTACTGAAGAAAAGCTCTTGGATCTTGATGCACTTCGG
AAAGCCAGTTCCGAAGGTAAATCGGAAATGAAGAAACTTAGACAGCAGCTTGAGGCAGCT
GAGAAACAGATTAAACATTTAGAGATTGAAAAGAATGCTGAAAGTAGCAAGGCTAGTAGC
ATTACCAGAGAGCTCCAGGGGAGAGAGCTAAAGCTTACTAACCTTCAGGAAAATTTGAGT
GAAGTCAGTCAAGTGAAAGAGACTTTGGAAAAAGAACTTCAGATTTTGAAAGAAAAGTTT
GCTGAAGCTTCAGAGGAGGCAGTCTCTGTTCAGAGAAGTATGCAAGAAACTGTAAATAAG
TTACACCAAAAGGAGGAACAGTTTAACATGCTGTCTTCTGACTTGGAGAAGCTGAGAGAA
AACTTAGCAGATATGGAGGCAAAATTTAGAGAGAAAGATGAGAGAGAAGAGCAGCTGATA
AAGGCAAAGGAAAAACTGGAAAATGACATTGCAGAAATAATGAAGATGTCAGGAGATAAC
TCTTCTCAGCTGACAAAAATGAACGATGAATTACGTCTGAAAGAAAGAGATGTAGAAGAA
TTACAGCTAAAACTTACAAAGGCTAATGAAAATGCAAGTTTTCTGCAAAAAAGTATTGAG
GACATGACTGTCAAAGCTGAACAGAGCCAGCAAGAAGCAGCTAAAAAGCATGAGGAAGAA
AAGAAAGAATTGGAGAGGAAATTGTCGGACCTGGAAAAGAAAATGGAAACAAGCCACAAC
CAGTGTCAGGAGCTGAAAGCCAGGTATGAGAGAGCCACTTCTGAGACAAAAACCAAGCAT
GAAGAAATCCTACAGAACCTCCAGAAGACGCTGCTGGACACAGAGGACAAGCTGAAGGGC
GCACGGGAGGAGAACAGTGGCTTGCTGCAGGAGCTGGAGGAGCTGAGAAAGCAAGCCGAC
AAAGCCAAAGCTGCTCAAACAGCGGAAGATGCCATGCAGATAATGGAACAGATGACCAAA
GAGAAGACTGAGACTCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAAACTACAG
AATGAATTGGACACACTTAAAGAAAACAACTTGAAAAATGTGGAAGAGCTGAACAAATCA
AAAGAACTCCTGACTGTAGAGAATCAAAAAATGGAAGAATTTAGGAAAGAAATAGAAACC
CTAAAGCAGGCAGCAGCTCAGAAGTCCCAGCAGCTTTCAGCGTTGCAAGAAGAGAACGTT
AAACTTGCTGAGGAGCTGGGGAGAAGCAGGGACGAAGTCACAAGTCATCAAAAGCTGGAA
GAAGAAAGATCTGTGCTCAATAATCAGTTGTTAGAAATGAAAAAAAGAGAATCCAAGTTC
ATAAAAGACGCAGATGAAGAGAAAGCTTCCTTGCAGAAATCCATCAGTATAACTAGTGCC
TTACTCACAGAAAAGGATGCCGAGCTGGAGAAACTGAGAAATGAGGTCACAGTGCTCAGG
GGAGAAAACGCCTCTGCCAAGTCCTTGCATTCAGTTGTTCAGACTCTAGAGTCTGATAAG
GTGAAGCTCGAGCTCAAGGTAAAGAACTTGGAGCTTCAACTCAAAGAAAACAAGAGGCAG
CTCAGCAGCTCCTCAGGTAATACAGACACTCAGGCAGACGAGGATGAAAGAGCCCAGGAG
AGTCAGATTGATTTCCTAAATTCAGTAATAGTGGACCTTCAAAGGAAGAATCAAGACCTC
AAGATGAAGGTGGAGATGATGTCAGAAGCAGCCCTGAATGGGAACGGGGATGACCTAAAC
AATTATGACAGTGATGATCAGGAGAAACAGTCCAAGAAGAAACCTCGCCTCTTCTGTGAC
ATTTGTGACTGCTTTGATCTCCACGACACAGAGGATTGTCCTACCCAGGCACAGATGTCA
GAGGACCCTCCCCATTCCACACACCATGGCAGTCGGGGTGAGGAACGCCCATACTGTGAA
ATCTGTGAGATGTTTGGACACTGGGCCACCAACTGCAATGACGACGAAACCTTC
Protein sequence predicted based on nucleotide sequence
MSMLKPSGLKAPTKILKPGSTALKTPTAVVAPVEKTISSEKASSTPSSETQEEFVDDFRV GERVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTR PSKLTRKVQAEDEANGLQTTPASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPS ATPPISNLTKTASESISNLSEAGSIKKGERELKIGDRVLVGGTKAGVVRFLGETDFAKGE WCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKANAVRRVM ATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGTTALQEALKEK QQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAA DREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEQKSQISEDPENTQTKLEHARIKEL EQSLLFEKTKADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESNKPAG DVDMSLSLLQEISSLQEKLEVTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSK ENESLKSKLEHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGLGTETAEFAELK TQIEKMRLDYQHEIENLQNQQDSERAAHAKEMEALRAKLMKVIKEKENSLEAIRSKLDKA EDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQTKVIDNFTSQLKATEEKLLDLDALR KASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLS EVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRE NLADMEAKFREKDEREEQLIKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERDVEE LQLKLTKANENASFLQKSIEDMTVKAEQSQQEAAKKHEEEKKELERKLSDLEKKMETSHN QCQELKARYERATSETKTKHEEILQNLQKTLLDTEDKLKGAREENSGLLQELEELRKQAD KAKAAQTAEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKS KELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLE EERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLR GENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQE SQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCD ICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
