Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00516399
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00720016
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3036nts
Open reading frame: 1 to 3036
ATGACAAAGCATCCACCTAACAGACGAGGAATCAGCTTTGAAGTGGGAGCCCAGTTGGAA
GCCCGGGACCGTTTAAAAAACTGGTATCCAGCTCACATAGAAGACATTGACTACGAGGAA
GGAAAAGTACTCATCCATTTCAAGCGTTGGAACCATCGTTATGATGAGTGGTTCTGCTGG
GACAGTCCTTATTTACGCCCTTTAGAGAAAATACAGCTGAGGAAAGAGGGCTTGCATGAA
GAGGATGGATCTTCTGAATTTCAAATAAATGAGCAGGTCCTTGCTTGCTGGTCTGATTGT
CGTTTTTACCCGGCCAAAGTCACTGCTGTTAACAAGGATGGTACTTACACTGTGAAATTT
TATGATGGAGTAGTTCAGACTGTCAAACATATTCATGTCAAAGCTTTTTCCAAAGATCAG
AATATTGTGGGTAATGCTAGGCCTAAAGAAACAGATCACAAAAGTCTTTCATCATCTCCT
GATAAACGAGAGAAGTTTAAAGAACAGAGAAAAGCAACAGTGAATGTGAAGAAAGACAAA
GAAGATAAACCCTTAAAGACAGAAAAGCGACCCAAGCAGCCTGATAAAGAAGGAAAGTTA
ATCTGTTCTGAAAAGGGGAAAGTGTCAGAGAAAAGTCTTCCCAAGAACGAGAAGGAAGAC
AAGGAAAACATTTCCGAAAATGACAGAGAGTATTCTGGAGATGCCCAAGTGGATAAGAAA
CCTGAAAATGACATTGTGAAGAGTCCACAAGAAAACTTGAGGGAACCCAAAAGAAAACGA
GGCAGACCCCCTTCCATAGCTCCTACTGCTGTGGATTCAAACTCTCAAACTTTGCAACCA
ATAACATTGGAACTGAGAAGAAGGAAAATATCAAAAGGATGTGAAGTCCCATTAAAACGT
CCTCGGCTTGACAAAAATTCATCCCAGGAAAAGTCAAAAAACTACTCGGAAAACACTGAC
AAAGACTTATCGAGGAGACGTTCCTCCAGGCTGTCCACTAATGGGACCCATGAGATCCTA
GATCCTGACTTGGTTGTATCAGATTTGGTTGATACGGATCCTTTGCAAGACACGTTGTCT
AGTACCAAGGAATCTGAAGAAGGTCAGTTGAAGTCTGCTTTGGAAGCTGGCCAGGTCTCA
TCTGCACTGACTTGCCACTCCTTTGGGGATGGATCCGGGGCTGCAGGCTTGGAGTTGAAC
TGCCCATCAATGGGAGAAAACACGATGAAAACAGAACCGACTTCTCCCCTTGTGGAATTA
CAAGAGATTTCGACTGTGGAAGTAACAAATACTTTTAAGAAAACAGATGATTTTGGGTCA
TCTAATGCACCAGCTGTCGACCTAGACCATAAGTTTAGATGCAAAGTTGTGGACTGTTTA
AAATTTTTCCGCAAAGCCAAACTGTTGCACTATCACATGAAGTATTTCCATGGAATGGAG
AAGTCACTGGAGCCAGAAGAGAGCCCGGGAAAGAGGCATGTCCAAACCAGGGGCCCTTCA
GCTTCAGACAAGCCCAGCCAGGAGACCCTGACCAGGAAGCGGGTCTCTGCCAGTTCCCCA
ACTACAAAAGACAAGGAAAAGAATAAAGAGAAGAAATTCAAGGAGTTTGTGAGAGTGAAG
CCAAAGAAGAAAAAGAAAAAGAAAAAGAAAACCAAACCTGAATGCCCCTGCAGTGAGGAG
ATCAGTGACACCTCCCAGGAACCTTCTCCACCCAAGGCATTTGCTGTTACCAGGTGTGGG
TCCTCACACAAGCCAGGGGTCCATATGAGCCCGCAGCTTCATGGCCCAGAATCTGGACAC
CACAAAGGGAAAATGAAAGCATTGGAGGAGGATAATTTGAGTGAGTCCTCTTCTGAGAGC
TTTCTCTGGAGTGATGATGAGTATGGCCAAGATGTGGATGTGACCACCAACCCAGATGAG
GAACTTGATGGGGATGACCGCTATGACTTCGAGGTGGTCCGCTGCATCTGTGAGGTCCAG
GAGGAAAATGACTTCATGATTCAGTGTGAAGAGTGCCAGTGCTGGCAGCATGGGGTCTGC
ATGGGATTACTGGAAGAAAATGTGCCCGAGAAATACACCTGTTATGTTTGCCAAGACCCT
CCAGGTCAGAGGCCTGGCTTCAAGTACTGGTATGACAAGGAGTGGCTGAGCAGGGGACAT
ATGCATGGCCTGGCATTTCTAGAAGAGAACTACTCCCATCAGAATGCCAAGAAGATCGTG
GCCACCCACCAGCTTCTTGGTGATGTGCAGAGAGTGATTGAGGTTCTGCATGGCCTGCAG
CTCAAGATGAGCATCTTGCAAAGCCGGGAGCATCCTGATCTGCCGCTGTGGTGCCAGCCT
TGGAAACAGCACTCAGGGGAGGGGAGATCTCATTTCAGAAACATCCCTGTCACTGACACC
AGGAGCAAGGAGGAAGCTCCAAGCTATAGAACTTTGAACGGGGCAGTGGAGAAGCCCAGG
CCCCTGGCCCTGCCCCTGCCGCGTTCTGTGGAGGAATCCTATATCACCAGTGAGCATTGC
TACCAGAAGCCCCGCGCCTATTACCCTGCCGTGGAGCAGAAGCTGGTGGTGGAGACGAGG
GGCTCTGCCCTCGACGATGCGGTCAACCCCCTCCATGAGAACGGCGATGATTCCCTTTCC
CCGCGCCTGGGCTGGCCTCTAGACCAAGACAGGAGCAAGGGGGACAGTGACCCCAAACCC
GGCTCCCCAAAGGTGAAGGAATATGTCTCCAAAAAGGCCCTACCAGAAGAAGCCCCTGCT
CGGAAGCTGCTGGACAGAGGTGGAGAGGGGCTGCTGAGCTCCCAGCACCAGTGGCAGTTT
AACCTGCTGACCCATGTGGAATCTCTTCAGGATGAAGTTACGCACAGGATGGACTCCATT
GAGAAGGAGTTGGATGTGTTGGAGAGCTGGCTGGACTACACTGGGGAACTGGAGCCCCCT
GAGCCGCTGGCCAGGCTTCCGCAGCTCAAGCATTGTATCAAGCAGCTGCTGATGGACCTG
GGCAAGGTGCAGCAGATCGCCCTCTGCTGCTCAACA
Protein sequence predicted based on nucleotide sequence
MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGKVLIHFKRWNHRYDEWFCW DSPYLRPLEKIQLRKEGLHEEDGSSEFQINEQVLACWSDCRFYPAKVTAVNKDGTYTVKF YDGVVQTVKHIHVKAFSKDQNIVGNARPKETDHKSLSSSPDKREKFKEQRKATVNVKKDK EDKPLKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEKEDKENISENDREYSGDAQVDKK PENDIVKSPQENLREPKRKRGRPPSIAPTAVDSNSQTLQPITLELRRRKISKGCEVPLKR PRLDKNSSQEKSKNYSENTDKDLSRRRSSRLSTNGTHEILDPDLVVSDLVDTDPLQDTLS STKESEEGQLKSALEAGQVSSALTCHSFGDGSGAAGLELNCPSMGENTMKTEPTSPLVEL QEISTVEVTNTFKKTDDFGSSNAPAVDLDHKFRCKVVDCLKFFRKAKLLHYHMKYFHGME KSLEPEESPGKRHVQTRGPSASDKPSQETLTRKRVSASSPTTKDKEKNKEKKFKEFVRVK PKKKKKKKKKTKPECPCSEEISDTSQEPSPPKAFAVTRCGSSHKPGVHMSPQLHGPESGH HKGKMKALEEDNLSESSSESFLWSDDEYGQDVDVTTNPDEELDGDDRYDFEVVRCICEVQ EENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGH MHGLAFLEENYSHQNAKKIVATHQLLGDVQRVIEVLHGLQLKMSILQSREHPDLPLWCQP WKQHSGEGRSHFRNIPVTDTRSKEEAPSYRTLNGAVEKPRPLALPLPRSVEESYITSEHC YQKPRAYYPAVEQKLVVETRGSALDDAVNPLHENGDDSLSPRLGWPLDQDRSKGDSDPKP GSPKVKEYVSKKALPEEAPARKLLDRGGEGLLSSQHQWQFNLLTHVESLQDEVTHRMDSI EKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLMDLGKVQQIALCCST
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
