Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00303029
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00731633
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4965nts
Open reading frame: 1 to 4965
ATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGG
CACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAG
CTGCTGCTCGACGCCAACCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAAC
TTGCGCAAGCTGGGCCTGAGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAAC
TTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGAGC
ATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTC
CCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTG
CAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAG
AACCTGCTCAAGTCCCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGAT
CTGGGAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGG
GAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGG
CGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGAGGAGCTGCCTGCTGAGCTCGGC
GGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGAC
GGCATCGGTCAGCTGAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAG
GTGACTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTG
CTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGAC
CGGAACCACCTCGAGGCGCTGCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTC
TCCTTGAGGGACAACCGCCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTG
CACGTGCTGGACGTGGCGGGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTC
AATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGACG
GAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCC
CCACTCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCC
CCGCCGAGCCGCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCT
GAGGAAGCTGCAGCTGAGAAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAG
CTCAAGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGCCA
GACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTG
AGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGAGGGCCCGTCG
GCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCC
GAAGAGGACTACCAGGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGAT
GACAGGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAG
CGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCC
CAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCATGCAGCCTGATGGGGAGGGCCCTGTG
GCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAG
GAGGAGGAGGAGGAAGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGAGGAGGAAAAC
AGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCT
GCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCT
CGCATTGAGGAGGAAGAGCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATC
AGCATTGCGGGCGGCAAGGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATC
TCTCGGGTGTCCGAGGAAGGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTC
CTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGCTC
CGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAG
AACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGG
GGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGC
CACGTGGCCTGCCTGGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAA
GGCTCCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGC
GGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGA
GTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTCCCTGCTGACCGCTGCCTCCCCC
ACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCA
CATTCCTCACCCCCCACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGG
GTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATAC
CCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGA
GGCTCCGACCATTCCAGCCACCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAG
GTGCTCCCGCGGGGCCTGGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCA
GTGAACGGGCAAGACGTGCGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGG
CCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGGAA
CTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGG
GGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGC
CCCACGGGGGCAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTG
AACCAGCAGAGCCTGCTGGGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTG
GGCGACACCCTCACCGTGCTGGTCTGTGACGGCTTCGAGGCCAGCACCGACGCAGCCCTG
GAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGC
CTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCCTGAGGGCCCAGGCAAGGAGAAG
GAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGT
CTGCAGCCCCTGAAGCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTG
CAGAGGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGT
GGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCC
TACAGGGCCTTCGCGGCCGTGCCCACTTCTCACCCGCCTGAGGATGCCCCTGCCCAGCCC
CCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTAC
TTTGAGCTGGAGGTGCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTG
GGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGA
GCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGG
GAGACGCTGGGCGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCC
ACCTCAAGGCAGAGCCCGGCGTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACG
GCCAAAGCTGAACGGCGCCACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCA
CCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGCTG
TGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTC
AGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCT
TCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACC
TCCCCGGGACGCCTGCCCTTGTCTGGAAAGAAGTTTGACTACAGGGCCTTTGCGGCCCTG
CCTTCTTCCAGACCTGTCTATGACATCCAGTCACCGGACTTTGCTGAGGAGTTGAGGTCC
CTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGAGGATGGAGAAGTGGCTCTGGTGCTT
CTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTGAAGATGTGGCACTGTGCAGCAGCCGC
CGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCC
Protein sequence predicted based on nucleotide sequence
MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLN LRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRL PDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLD LGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELG GLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENL LMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTEL HVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQP PLSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSE LKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPS AEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQ RLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKE EEEEEEGSPQEEEEEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPA RIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKL LEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERR GGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEG GAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLP HSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVG GSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLR PCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVS PTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAAL EVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRG LQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQA YRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLV GADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSP TSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRAL WRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTST SPGRLPLSGKKFDYRAFAALPSSRPVYDIQSPDFAEELRSLEPSPSPGPQEEDGEVALVL LGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
