DNASU Plasmid Repository • 480.965.2857 | Email

BCL2 (Homo sapiens) in pcDNA3

TOP

Explanation of Terms


Gene: Gene Symbol:  BCL2
Gene Name:  B-cell CLL/lymphoma 2
Sequence              Map: 3336.pdf
Original Clone ID: 3336
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pcDNA3               Format:  CLOSED
Source: Victoria Moore
Description: Expression of wild-type human BCL-2 in mammalian cells; amp resistance (neomycin for mammalian cells); restriction enzyme cloning.
Comments: None
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 10567572
Title: BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M
PMID: 8183370
Title: BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax
PMID: 2834197
Title: Alternative promoters and exons, somatic mutation and deregulation of the Bcl-2-Ig fusion gene in lymphoma
Authors: NA Seto
NA Yamamoto
NA Yin
Stanley Korsmeyer
Tony Letai
Victoria Moore
HsCD00073509

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  neomycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pcDNA3

pcDNA3 in advanced viewed

Synonyms: pcDNA-3
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector with CMV promoter, BGH polyA signal, T7, SP6; amp resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: None
Size (bp): 5446
Parent Vector: None
Empty Vector: None
Properties: in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (HindIII KpnI BamHI BstXI EcoRI EcoRV BstXI NotI XhoI XbaI ApaI) 0 0
bacterial origin ColE1 ColE1 origin of replication 0 0
poly-A signal SV40 polyA SV40 polyadenylation signal sequence (for neoR) 0 0
poly-A signal BGH polyA BGH polyadenylation signal sequence 0 0
promoter SP6 SP6 prokaryotic promoter 0 0
promoter T7 T7 prokaryotic promoter 0 0
promoter CMV CMV promoter 0 0
promoter SV40 SV40 promoter (for neoR) 0 0
selectable marker AmpR ampicillin resistance 0 0
selectable marker NeoR neomycin resistance gene 0 0
viral origin SV40 SV40 origin (with SV40 promoter) 0 0

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : BCL2
Symbol Nomenclature : BCL2
SYNONYM : Bcl-2; PPP1R50
Designation : apoptosis regulator Bcl-2|protein phosphatase 1, regulatory subunit 50
Full Nomenclature : B-cell CLL/lymphoma 2
GENEID : 596
SYNONYM : wt human flag-BCL-2
HGNC : 990
MIM : 151430
Target GenBank: None

 

Pathways

NCI : ATF-2 transcription factor network
NCI : C-MYB transcription factor network
NCI : Caspase Cascade in Apoptosis
NCI : Ceramide signaling pathway
NCI : Direct p53 effectors
NCI : EPO signaling pathway
NCI : HIV-1 Nef: Negative effector of Fas and TNF-alpha
NCI : IL2 signaling events mediated by PI3K
NCI : IL2 signaling events mediated by STAT5
NCI : IL2-mediated signaling events
NCI : RXR and RAR heterodimerization with other nuclear receptor
NCI : Role of Calcineurin-dependent NFAT signaling in lymphocytes
NCI : Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI : Validated targets of C-MYC transcriptional repression
Panther : Apoptosis signaling pathway
Panther : Oxidative stress response
Reactome : Activation of BAD and translocation to mitochondria
Reactome : Activation of BH3-only proteins
Reactome : Apoptosis
Reactome : BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members
Reactome : Immune System
Reactome : Inflammasomes
Reactome : Innate Immune System
Reactome : Intrinsic Pathway for Apoptosis
Reactome : Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways
Reactome : The NLRP1 inflammasome
WikiPathway : Apoptosis
WikiPathway : Apoptosis Modulation and Signaling
WikiPathway : DNA damage response (only ATM dependent)
WikiPathway : Estrogen signaling pathway
WikiPathway : FAS pathway and Stress induction of HSP regulation
WikiPathway : Focal Adhesion
WikiPathway : IL-2 Signaling pathway
WikiPathway : IL-3 Signaling Pathway
WikiPathway : IL-5 signaling pathway
WikiPathway : Integrated Breast Cancer Pathway
WikiPathway : Integrated Cancer pathway
WikiPathway : Kit receptor signaling pathway
WikiPathway : Leukocyte TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase
WikiPathway : Senescence and Autophagy
WikiPathway : TP53 network

Protein

HPRD : 01045

Clone

Original Clone ID : 3336

Organism


TAX_ID : 9606
Species Specific ID: 596

Genome

Chromosome : 18
Map Location : 18q21.3
Ensembl : ENSG00000171791