Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744544
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5403nts
Open reading frame: 1 to 5403
ATGGCTGTGCCCGGCTCCTTCCCGCTGCTGGTCGAGGGCTCCTGGGGACCCGACCCCCCG
AAAAACTTAAACACCAAGTTGCAGATGTACTTCCAGAGCCCGAAGAGGTCTGGAGGCGGA
GAGTGTGAGGTCCGGCAGGATCCCAGGAGCCCATCACGCTTCCTGGTCTTCTTCTACCCG
GAGGACGTTCGACAGAAGGTTCTGGAGAGAAAAAATCATGAGTTGGTATGGCAAGGAAAA
GGAACATTCAAGTTAACTGTCCAGTTACCTGCAACCCCAGATGAAATCGATCATGTCTTT
GAAGAGGAACTTCTAACAAAAGAATCTAAGACCAAAGAAGATGTTAAAGAACCAGATGTG
TCAGAAGAACTGGATACAAAACTCCCTCTTGATGGTGGATTAGACAAAATGGAAGATATC
CCAGAGGAATGTGAAAATATTTCCTCTTTGGTGGCGTTTGAAAATCTCAAGGCAAATGTC
ACTGACATAATGCTAATCTTGTTAGTGGAGAACATAAGTGGCCTGTCTAATGATGACTTT
CAAGTGGAAATAATACGAGACTTTGATGTTGCTGTTGTTACCTTTCAAAAGCACATAGAT
ACTATAAGATTCGTTGATGATTGCACCAAGCACCATTCAATTAAACAACTTCAGCTATCT
CCAAGACTTCTGGAAGTCACAAACACAATCAGGGTTGAAAACCTGCCACCTGGTGCCGAT
GACTACAGTTTAAAACTTTTCTTTGAAAATCCCTATAATGGAGGCGGGAGAGTTGCCAAT
GTTGAATATTTTCCTGAAGAGAGCTCAGCTCTGATTGAATTTTTTGACAGAAAAGTGTTG
GACACCATCATGGCCACAAAACTTGACTTCAATAAAATGCCACTTTCTGTGTTCCCATAC
TATGCCTCATTGGGCACAGCCTTGTATGGAAAGGAGAAGCCTCTGATCAAGCTTCCAGCA
CCATTTGAAGAGTCCCTAGATCTTCCTTTATGGAAGTTCTTACAGAAAAAGAATCACCTT
ATTGAGGAGATAAACGATGAAATGAGGCGTTGTCACTGTGAGCTCACGTGGTCACAACTC
TCTGGTAAAGTTACAATCAGACCAGCAGCCACTTTAGTCAATGAAGGAAGACCGAGAATT
AAGACCTGGCAGGCTGATACTTCTACAACACTCTCTAGCATCAGGTCAAAGTATAAAGTC
AACCCAATTAAAGTGGATCCAACAATGTGGGACACCATAAAAAATGATGTCAAGGATGAC
AGGATTTTGATTGAGTTTGATACACTTAAGGAGATGGTAATCTTAGCAGGGAAATCAGAG
GATGTCCAATCTATTGAGGTACAAGTCAGAGAGTTAATAGAATCCACTACTCAAAAAATT
AAAAGGGAAGAGCAAAGTCTCAAGGAAAAAATGATCATTTCTCCCGGCAGATATTTTCTT
TTGTGTCACTCTTCTCTACTCGATCACTTACTCACGGAGTGCCCAGAGATAGAGATTTGT
TACGATAGGGTCACTCAACACTTGTGCCTGAAAGGACCTAGTGCCGATGTGTATAAAGCA
AAGTGTGAAATCCAGGAGAAGGTGTACACCATGGCTCAGAAAAACATTCAGGTTTCCCCT
GAGATTTTTCAATTTTTGCAACAGGTAAACTGGAAAGAATTCTCTAAGTGTCTTTTCATA
GCACAGAAGATTCTTGCACTTTATGAGCTAGAGGGTACAACTGTTCTCTTAACCAGCTGT
TCTTCTGAAGCCCTGTTAGAGGCAGAAAAGCAAATGCTCTCTGCCTTGAATTATAAGCGC
ATTGAAGTTGAGAACAAAGAAGTTCTTCATGGCAAGAAATGGAAAGGGCTCACTCACAAT
TTGCTTAAGAAACAAAATTCTTCCCCAAACACTGTAATCATCAATGAGTTAACTTCAGAA
ACCACAGCTGAAGTCATCATCACCGGATGTGTAAAAGAAGTAAACGAAACCTATAAACTC
CTTTTTAACTTCGTTGAACAAAACATGAAAATAGAGAGACTGGTTGAAGTAAAGCCCTCC
TTAGTTATTGACTATTTGAAGACAGAAAAGAAGCTATTCTGGCCAAAGATAAAGAAGGTA
AATGTGCAGGTAAGTTTCAATCCTGAGAACAAACAAAAAGGCATTCTACTAACTGGCTCA
AAGACCGAAGTACTGAAGGCAGTGGACATTGTCAAGCAAGTCTGGGACTCAGTCTGTGTT
AAAAGCGTCCATACTGATAAGCCAGGAGCCAAGCAGTTCTTCCAGGATAAAGCACGGTTT
TATCAAAGTGAGATCAAACGGTTGTTTGGTTGTTACATTGAACTACAGGAGAATGAAGTG
ATGAAGGAGGGAGGCAGCCCCGCTGGGCAGAAGTGCTTCTCTCGGACAGTGCTGGCCCCT
GGAGTTGTGCTGATCGTGCAGCAGGGTGACTTGGCACGGCTTCCTGTCGATGTGGTGGTG
AATGCATCTAATGAGGACCTTAAGCATTATGGCGGCCTGGCAGCTGCGCTCTCAAAAGCA
GCTGGCCCTGAGCTCCAGGCAGACTGTGACCAGATAGTGAAGAGAGAGGGCAGACTCCTA
CCGGGCAATGCCACCATCTCCAAGGCAGGGAAGCTCCCCTACCACCACGTGATCCATGCA
GTGGGGCCCCGCTGGAGCGGGTATGAGGCCCCGAGGTGTGTGTACCTATTAAGGAGAGCC
GTGCAACTCAGTCTGTGTCTAGCCGAAAAATACAAGTACCGATCCATAGCCATCCCAGCT
ATTAGTTCTGGAGTGTTTGGCTTTCCCTTAGGCCGATGCGTGGAGACAATTGTTTCTGCC
ATCAAGGAAAACTTCCAATTCAAGAAGGATGGACACTGCTTGAAAGAAATCTACCTTGTG
GATGTATCAGAGAAAACTGTTGAAGCCTTTGCAGAAGCTGTGAAAACTGTATTTAAAGCC
ACCCTGCCCGATACAGCTGCCCCGCCAGGCTTACCACCAGCAGCAGCGGGGCCTGGGAAA
ACATCATGGGAAAAAGGAAGCCTGGTGTCCCCGGGAGGCCTGCAGATGCTGTTGGTGAAA
GAGGGCGTGCAGAATGCCAAGACAGATGTTGTTGTCAACTCCGTTCCCTTGGATCTGGTG
CTTAGCAGAGGGCCTCTTTCGAAGTCCCTCTTGGAAAAAGCTGGGCCAGAGCTGCAGGAG
GAATTGGACACAGTTGGACAAGGGGTGGCTGTCAGCATGGGCACAGTGCTCAAAACCAGC
AGCTGGAATCTGGACTGTCGCTATGTACTTCACGTGGTAGCTCCGGAGTGGAGAAATGGT
AGCACATCTTCACTCAAGATAATGGAAGACATAATCAGAGAATGTATGGAGATCACTGAG
AGCTTGTCATTAAAATCAATTGCCTTTCCAGCAATAGGAACAGGAAACTTGGGATTTCCT
AAAAACATATTCGCTGAATTAATCATTTCAGAGGTGTTCAAATTTAGCAGCAAGAATCAG
CTGAAAACTTTACAAGAGGTTCACTTTCTGCTCCACCCGAGTGATCATGAAAATATTCAG
GCATTTTCAGATGAATTTGCCAGAAGGGCTAATGGAAATCTCGTTAGTGACAAAATTCCG
AAGGCCAAAGATACACAAGGCTTTTATGGGACCGTTTCTAGTCCTGATTCCGGTGTGTAT
GAAATGAAGATTGGCTCCATTATTTTCCAGGTGGCTTCTGGAGATATCACGAAAGAAGAG
GCAGATGTGATTGTGAATTCAACATCAAACTCATTCAATCTCAAAGCCGGGGTGTCCAAA
GCAATTTTAGAGTGTGCTGGCCAAAATGTTGAAAGGGAATGTTCTCAGCAAGCTCAGCAG
CGGAAAAATGATTATATAATCACGGGAGGTGGCTTTCTGAGGTGCAAGAATATCATTCAT
GTAATTGGTGGAAATGATGTCAAGAGCTCAGTTTCCTCTGTTTTGCAGGAGTGTGAAAAA
AAGAATTACTCATCTATTTGCCTCCCAGCCATTGGGACAGGAAATGCCAAACAACACCCA
GATAAGGTTGCTGAAGCCATAATTGATGCCATTGAAGACTTTGTCCAGAAAGGATCAGCC
CAGTCCGTGAAAAAAGTTAAAGTTGTTATCTTTCTGCCTCAAGTACTGGATGTGTTTTAT
GCCAATATGAAGAAAAGAGAAGGCACCCAGCTTTCTTCTCAACAGTCTGTGATGTCAAAA
CTTGCTTCATTTTTGGGCTTTTCAAAGCAATCTCCCCAAAAAAAGAATCATTTGGTTTTG
GAAAAGAAAACCGAATCTGCAACTTTTCGGGTGTGTGGTGAAAATGTCACCTGTGTGGAA
TATGCTATCTCCTGGCTACAAGACCTGATTGAAAAAGAACAGTGTCCTTACACCAGTGAA
GATGAGTGCATCAAAGACTTTGACGAAAAGGAGTATCAGGAGTTGAATGAGCTGCAGAAG
AAGTTAAATATTAACATTTCCCTGGACCATAAGAGGCCTTTGATTAAGGTTTTGGGAATT
AGCAGAGATGTGATGCAGGCTAGAGATGAAATTGAGGCGATGATCAAGAGAGTGCGATTG
GCCAAAGAACAGGAATCCCGGGCAGATTGTATCAGTGAATTTATAGAATGGCAGTATAAT
GACAATAACACTTCCCACTGTTTTAACAAAATGACCAATCTGAAATTAGAAGATGCCAGG
AGGGAAAAGAAAAAGACAGTTGATGTCAAAATTAATCATCGGCACTACACAGTGAACCTC
AACACATACACTGCCACAGACACAAAGGGCCACAGTTTATCTGTTCAGCGCCTCACGAAA
TCCAAAGTGGACATCCCCGCACACTGGAGCGATATGAAGCAGCAGAATTTCTGTGTGGTA
GAGCTGCTGCCTAGTGATCCTGAGTACAACACGGTGGCAAGCAAGTTTAACCAGACCTGC
TCGCACTTCAGAATAGAGAAGATTGAGAGGATCCAGAATCCAGATCTCTGGAATAGCTAC
CAGGCAAAGAAAAAAACTATGGATGCCAAGAATGGCCAGACAATGAATGAAAAGCAACTC
TTCCATGGGACAGATGCCGGCTCCGTGCCACACGTCAATCGAAATGGCTTTAACCGCAGC
TATGCCGGAAAGAATGCTGTAGCATATGGAAAGGGAACCTATTTTGCTGTCAATGCCAAT
TATTCTGCCAATGATACGTACTCCAGACCTGATGCAAATGGGCGAAAGCATGTGTATTAT
GTGCGAGTACTTACTGGGATCTATACCCATGGAAATCATTCATTGATTGTGCCTCCTTCA
AAGAACCCTCAAAATCCTACTGATCTGTATGACACTGTCACAGATAATGTGCACCATCCA
AGCTTATTTGTGGCATTTTATGACTACCAAGCATACCCAGAGTACCTTATTACGTTTAGA
AAA
Protein sequence predicted based on nucleotide sequence
MAVPGSFPLLVEGSWGPDPPKNLNTKLQMYFQSPKRSGGGECEVRQDPRSPSRFLVFFYP EDVRQKVLERKNHELVWQGKGTFKLTVQLPATPDEIDHVFEEELLTKESKTKEDVKEPDV SEELDTKLPLDGGLDKMEDIPEECENISSLVAFENLKANVTDIMLILLVENISGLSNDDF QVEIIRDFDVAVVTFQKHIDTIRFVDDCTKHHSIKQLQLSPRLLEVTNTIRVENLPPGAD DYSLKLFFENPYNGGGRVANVEYFPEESSALIEFFDRKVLDTIMATKLDFNKMPLSVFPY YASLGTALYGKEKPLIKLPAPFEESLDLPLWKFLQKKNHLIEEINDEMRRCHCELTWSQL SGKVTIRPAATLVNEGRPRIKTWQADTSTTLSSIRSKYKVNPIKVDPTMWDTIKNDVKDD RILIEFDTLKEMVILAGKSEDVQSIEVQVRELIESTTQKIKREEQSLKEKMIISPGRYFL LCHSSLLDHLLTECPEIEICYDRVTQHLCLKGPSADVYKAKCEIQEKVYTMAQKNIQVSP EIFQFLQQVNWKEFSKCLFIAQKILALYELEGTTVLLTSCSSEALLEAEKQMLSALNYKR IEVENKEVLHGKKWKGLTHNLLKKQNSSPNTVIINELTSETTAEVIITGCVKEVNETYKL LFNFVEQNMKIERLVEVKPSLVIDYLKTEKKLFWPKIKKVNVQVSFNPENKQKGILLTGS KTEVLKAVDIVKQVWDSVCVKSVHTDKPGAKQFFQDKARFYQSEIKRLFGCYIELQENEV MKEGGSPAGQKCFSRTVLAPGVVLIVQQGDLARLPVDVVVNASNEDLKHYGGLAAALSKA AGPELQADCDQIVKREGRLLPGNATISKAGKLPYHHVIHAVGPRWSGYEAPRCVYLLRRA VQLSLCLAEKYKYRSIAIPAISSGVFGFPLGRCVETIVSAIKENFQFKKDGHCLKEIYLV DVSEKTVEAFAEAVKTVFKATLPDTAAPPGLPPAAAGPGKTSWEKGSLVSPGGLQMLLVK EGVQNAKTDVVVNSVPLDLVLSRGPLSKSLLEKAGPELQEELDTVGQGVAVSMGTVLKTS SWNLDCRYVLHVVAPEWRNGSTSSLKIMEDIIRECMEITESLSLKSIAFPAIGTGNLGFP KNIFAELIISEVFKFSSKNQLKTLQEVHFLLHPSDHENIQAFSDEFARRANGNLVSDKIP KAKDTQGFYGTVSSPDSGVYEMKIGSIIFQVASGDITKEEADVIVNSTSNSFNLKAGVSK AILECAGQNVERECSQQAQQRKNDYIITGGGFLRCKNIIHVIGGNDVKSSVSSVLQECEK KNYSSICLPAIGTGNAKQHPDKVAEAIIDAIEDFVQKGSAQSVKKVKVVIFLPQVLDVFY ANMKKREGTQLSSQQSVMSKLASFLGFSKQSPQKKNHLVLEKKTESATFRVCGENVTCVE YAISWLQDLIEKEQCPYTSEDECIKDFDEKEYQELNELQKKLNINISLDHKRPLIKVLGI SRDVMQARDEIEAMIKRVRLAKEQESRADCISEFIEWQYNDNNTSHCFNKMTNLKLEDAR REKKKTVDVKINHRHYTVNLNTYTATDTKGHSLSVQRLTKSKVDIPAHWSDMKQQNFCVV ELLPSDPEYNTVASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQL FHGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPDANGRKHVYY VRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSLFVAFYDYQAYPEYLITFR K
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
