Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745033
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6891nts
Open reading frame: 1 to 6891
ATGGACGGCGACGGTGGCAGGCGAGACGTGCCCGGCACCCTCATGGAACCCGGGCGGGGC
GCTGGACCCGCCGGCATGGCCGAACCTAGAGCTAAGGCCGCACGACCCGGGCCACAGCGC
TTTCTGCGGCGCAGTGTGGTCGAGTCCGACCAGGAAGAGCCCCCCGGCTTGGAGGCCGCT
GAGGCCCCCGGCCCACAGCCTCCGCAGCCCCTTCAACGCCGGGTCCTTCTGCTGTGTAAG
ACACGCCGCCTCATTGCCGAGCGCGCCAGAGGTCGTCCTGCCGCTCCCGCGCCCGCAGCT
CTTGTTGCCCAACCTGGCGCCCCTGGAGCTCCCGCTGACGCCGGTCCCGAACCTGTCGGC
ACCCAGGAGCCCGGACCTGACCCCATCGCCGCTGCTGTCGAAACAGCGCCTGCGCCTGAC
GGCGGCCCCAGGGAGGAGGCTGCGGCTACCGTGCGCAAGGAAGATGAGGGCGCCGCCGAG
GCTAAACCTGAACCCGGCCGTACCCGCAGAGACGAGCCCGAAGAGGAGGAGGACGACGAG
GACGATCTCAAAGCCGTGGCTACCTCTCTGGACGGCAGATTCCTGAAGTTCGACATCGAG
CTGGGCCGCGGATCCTTCAAGACCGTCTACAAGGGCCTCGACACCGAGACATGGGTGGAG
GTGGCCTGGTGTGAGCTGCAGGACCGGAAGCTGACAAAGCTGGAACGGCAGCGGTTCAAA
GAAGAGGCCGAAATGCTGAAAGGACTGCAGCACCCCAACATCGTGCGCTTCTACGACTTC
TGGGAGTCTAGCGCTAAGGGCAAGCGGTGCATCGTGCTGGTGACCGAGCTGATGACCTCC
GGTACTCTCAAGACCTACCTCAAGCGCTTCAAGGTGATGAAACCCAAGGTTCTGCGCAGC
TGGTGTCGACAGATTCTCAAGGGACTGCTTTTCCTGCATACCAGGACACCACCCATCATC
CACCGCGACCTGAAATGTGACAATATTTTCATTACCGGCCCAACTGGTTCTGTGAAGATT
GGCGATCTGGGACTGGCCACCCTGAAAAGAGCTTCATTTGCTAAAAGTGTGATTGGGACC
CCCGAGTTCATGGCTCCAGAGATGTACGAGGAGCACTACGATGAGTCCGTGGACGTCTAT
GCCTTTGGCATGTGCATGCTCGAGATGGCCACCAGCGAGTACCCCTACTCTGAGTGTCAG
AATGCCGCTCAGATCTACCGCAAAGTGACCTGTGGGATTAAGCCTGCCTCATTTGAGAAA
GTGCACGATCCTGAGATCAAGGAGATTATTGGCGAGTGTATCTGCAAAAACAAAGAGGAG
AGGTACGAGATCAAAGACCTGCTGAGCCACGCCTTCTTTGCAGAGGATACAGGCGTGAGG
GTGGAGCTCGCCGAGGAGGATCACGGGAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTG
GAAGACCCCAAGAAACTGAAGGGAAAGCCAAAGGACAATGGTGCCATCGAGTTCACCTTT
GACCTGGAGAAGGAGACACCCGATGAAGTGGCCCAGGAGATGATTGAGTCTGGATTCTTC
CACGAGAGTGATGTGAAAATCGTGGCTAAGTCTATCCGGGACCGCGTGGCCCTGATCCAG
TGGCGGCGGGAGAGGATCTGGCCCGCGCTGCAGCCTAAAGAGCAGCAAGATGTGGGATCC
CCCGACAAAGCCAGGGGACCGCCTGTGCCCCTGCAGGTCCAGGTGACCTACCACGCACAA
GCCGGACAGCCCGGCCCTCCTGAGCCCGAGGAACCCGAGGCCGACCAGCACCTTCTCCCA
CCAACCCTGCCAACCAGCGCTACCTCCCTAGCCTCTGACTCCACCTTCGACAGTGGCCAG
GGATCCACCGTGTACTCCGACTCTCAGAGCAGCCAGCAAAGCGTGATGCTCGGCTCCCTT
GCTGACGCAGCTCCGTCCCCTGCCCAGTGTGTGTGTTCCCCCCCTGTGAGCGAGGGCCCC
GTTCTGCCTCAGAGCCTGCCCTCCCTCGGCGCCTACCAGCAGCCTACAGCCGCACCTGGC
CTGCCCGTCGGCTCTGTGCCAGCCCCTGCCTGCCCCCCCTCCTTGCAGCAGCACTTCCCC
GATCCCGCCATGAGCTTCGCCCCTGTGCTGCCGCCTCCCTCTACCCCCATGCCAACGGGA
CCTGGACAGCCTGCACCCCCAGGCCAGCAACCTCCCCCACTGGCCCAGCCAACCCCTCTG
CCGCAGGTCCTCGCCCCCCAGCCCGTGGTCCCCCTTCAGCCCGTTCCACCCCATCTGCCT
CCTTACCTGGCACCAGCCAGCCAGGTGGGCGCCCCCGCTCAGCTGAAACCGCTTCAGATG
CCTCAGGCTCCCCTCCAGCCTCTCGCCCAGGTGCCTCCACAGATGCCACCTATTCCTGTT
GTGCCCCCCATCACCCCCCTGGCCGGCATCGACGGCCTCCCACCGGCCCTGCCAGACCTG
CCTACCGCTACCGTCCCCCCCGTGCCACCACCTCAGTATTTCTCTCCAGCAGTGATCCTT
CCCAGCCTCGCCGCTCCACTCCCTCCTGCATCCCCCGCATTGCCACTGCAAGCCGTTAAG
CTGCCCCATCCGCCTGGAGCGCCCCTAGCCATGCCCTGTAGGACAATTGTGCCAAATGCA
CCCGCCACCATCCCCCTGCTGGCCGTCGCCCCTCCCGGTGTGGCCGCCCTGTCCATTCAC
TCTGCCGTGGCACAGCTGCCCGGCCAACCTGTGTACCCAGCCGCCTTTCCTCAGATGGCC
CCTACTGACGTCCCTCCTTCCCCACATCACACAGTGCAGAATATGAGGGCTACCCCACCT
CAGCCGGCACTTCCACCACAGCCCACACTTCCGCCTCAGCCAGTGCTTCCTCCGCAACCT
ACGCTGCCCCCGCAGCCAGTGTTGCCCCCACAACCCACAAGGCCTCCTCAACCAGTGCTC
CCCCCTCAACCTATGCTGCCACCTCAACCCGTGCTTCCCCCTCAGCCCGCACTGCCTGTA
CGGCCTGAACCTCTCCAACCACACTTGCCTGAGCAGGCAGCCCCAGCTGCTACACCAGGG
AGCCAGATTCTGCTTGGCCATCCCGCTCCTTATGCTGTGGACGTGGCCGCACAGGTGCCA
ACCGTGCCCGTGCCTCCGGCCGCTGTGCTGTCACCTCCTCTGCCCGAAGTGCTTCTGCCC
GCCGCACCCGAACTCCTTCCTCAGTTCCCCAGCTCTCTGGCTACAGTGTCTGCATCCGTG
CAGAGTGTGCCCACACAGACCGCTACACTGCTGCCTCCCGCTAACCCTCCGCTGCCTGGC
GGGCCTGGGATCGCTAGCCCTTGCCCCACCGTGCAGCTGACCGTGGAACCAGTCCAGGAG
GAGCAGGCCTCACAGGACAAGCCTCCCGGACTGCCACAGAGCTGTGAAAGCTATGGCGGC
TCTGATGTCACTTCTGGCAAAGAGCTCAGCGATAGCTGTGAGGGAGCCTTTGGAGGGGGC
AGACTGGAAGGCAGAGCTGCCAGAAAACACCACCGGCGGTCCACCCGCGCTCGCTCTCGG
CAGGAGAGGGCCAGCCGGCCCCGGCTGACCATCCTGAACGTGTGCAACACCGGGGACAAA
ATGGTGGAGTGCCAGCTGGAGACACACAACCACAAGATGGTGACCTTCAAGTTTGACCTT
GACGGAGACGCCCCCGATGAGATTGCCACATATATGGTGGAACATGACTTTATCCTGCAG
GCCGAGCGAGAGACATTCATCGAACAGATGAAGGACGTCATGGACAAGGCCGAGGACATG
CTGAGCGAGGACACAGACGCCGACAGAGGCAGCGACCCAGGGACCAGCCCCCCACACCTG
AGCACTTGCGGACTCGGAACTGGCGAGGAGAGCAGACAGTCTCAGGCCAACGCTCCAGTG
TATCAGCAGAACGTGCTGCACACCGGCAAGAGGTGGTTCATCATCTGTCCCGTGGCCGAG
CACCCCGCACCAGAGGCCCCAGAATCTAGCCCTCCACTGCCTCTGAGCTCCCTGCCTCCA
GAGGCCAGCCAAGGCCCATGCAGAGGGCTCACCCTGCCTTGTCTCCCATGGCGCCGCGCA
GCCTGTGGGGCTGTGTTCCTGTCCCTGTTTTCTGCCGAGTCAGCCCAGTCCAAGCAGCCC
CCAGATTCCGCACCCTATAAAGACCAGCTGTCCTCCAAGGAACAGCCTAGCTTTCTGGCT
TCTCAGCAACTGCTGAGTCAGGCCGGACCCTCCAATCCACCTGGGGCACCCCCCGCCCCT
TTGGCCCCCTCATCCCCTCCTGTGACCGCTCTGCCCCAAGATGGAGCAGCTCCAGCCACC
AGTACCATGCCAGAACCCGCCTCCGGAACCGCTAGCCAGGCTGGCGGTCCTGGGACACCT
CAAGGCCTGACAAGCGAGCTCGAGACATCTCAGCCCCTCGCTGAGACTCACGAAGCCCCA
TTGGCAGTGCAACCCCTGGTGGTGGGACTTGCCCCTTGCACTCCAGCTCCCGAGGCTGCC
AGCACAAGGGACGCCAGTGCACCAAGGGAACCACTCCCACCTCCCGCCCCTGAGCCTAGC
CCCCACTCCGGGACACCCCAGCCTGCTCTGGGACAACCCGCTCCCCTGCTCCCCGCCGCT
GTTGGGGCCGTCTCCCTCGCCACAAGCCAGCTGCCAAGCCCACCACTGGGGCCCACCGTT
CCCCCACAGCCACCCTCGGCCCTGGAGTCTGATGGGGAGGGCCCGCCCCCAAGGGTCGGC
TTTGTGGACAGCACCATCAAGAGCCTGGACGAGAAGCTGAGGACCCTGCTGTACCAGGAG
CACGTGCCTACCTCCTCAGCCTCAGCTGGTACCCCTGTGGAAGTCGGCGATAGAGACTTC
ACCCTGGAACCCCTGAGAGGGGATCAGCCTAGATCAGAGGTGTGCGGTGGCGACCTGGCT
CTTCCCCCAGTGCCTAAGGAAGCCGTGTCAGGCAGAGTCCAGTTGCCCCAGCCACTCGTG
GAGAAGTCTGAGCTGGCCCCCACCCGAGGGGCCGTGATGGAACAGGGCACCTCTTCCTCT
ATGACAGCCGAGTCCTCTCCTAGAAGCATGCTGGGGTATGACAGAGATGGCAGACAGGTG
GCTTCAGACTCTCACGTGGTGCCCAGCGTCCCCCAGGATGTGCCTGCCTTTGTGCGCCCT
GCCCGCGTGGAGCCAACCGACAGGGATGGCGGAGAGGCCGGCGAGAGCAGCGCCGAACCC
CCCCCTAGCGACATGGGCACAGTGGGCGGACAGGCTAGCCACCCACAGACTCTGGGAGCC
CGCGCCTTGGGCTCTCCCCGGAAAAGGCCAGAACAGCAGGATGTCAGCTCACCAGCCAAA
ACCGTGGGAAGATTCTCCGTGGTGAGCACACAGGACGAGTGGACCCTGGCCTCCCCCCAT
AGCCTGAGATACTCTGCTCCACCTGACGTGTACCTCGACGAGGCCCCTTCCAGCCCAGAC
GTCAAGCTGGCAGTGAGGCGCGCCCAGACCGCCTCCTCTATCGAAGTGGGCGTGGGCGAA
CCCGTGTCCAGCGACTCTGGCGACGAGGGACCTAGGGCAAGACCCCCGGTCCAGAAGCAG
GCGTCTCTTCCCGTGAGTGGCAGCGTGGCTGGCGACTTCGTGAAGAAGGCTACCGCCTTC
CTGCAGAGGCCTTCTAGGGCCGGCAGCCTGGGTCCCGAGACACCTAGCAGGGTGGGCATG
AAGGTGCCCACCATCAGCGTGACTTCCTTCCATTCCCAGTCCTCCTACATCTCCTCCGAC
AATGATTCTGAGCTTGAGGATGCTGACATTAAGAAGGAGCTTCAGTCCCTGCGGGAGAAG
CACCTGAAGGAGATCTCTGAACTGCAGAGCCAGCAGAAACAGGAGATCGAAGCCCTGTAC
AGAAGGCTGGGCAAGCCATTACCCCCAAACGTCGGCTTCTTTCACACCGCCCCCCCCACC
GGGCGGAGGCGCAAAACCAGCAAGAGCAAGCTTAAGGCCGGCAAGCTGCTCAATCCCCTG
GTCCGGCAACTCAAGGTGGTTGCTTCCAGCACCGGCCACTTGGCCGACTCCTCTAGGGGT
CCTCCCGCAAAAGACCCGGCTCAGGCCTCCGTGGGACTGACTGCCGATAGCACCGGACTG
AGCGGAAAGGCTGTGCAGACCCAGCAGCCCTGCTCCGTCAGGGCCTCCCTGTCCTCTGAC
ATCTGCTCCGGCCTTGCCAGCGATGGAGGCGGAGCTAGAGGCCAAGGCTGGACCGTTTAC
CACCCAACTTCTGAGAGAGTGACCTATAAGTCCAGCAGCAAACCTCGCGCCCGGTTCCTG
AGTGGCCCCGTCTCAGTGTCCATCTGGTCTGCCCTGAAGAGACTCTGTCTCGGCAAGGAG
CACAGCAGCAGGTCATCCACTAGTAGCCTGGCCCCGGGCCCCGAGCCAGGCCCCCAACCC
GCCCTGCACGTGCAAGCCCAGGTGAACAACAGCAACAACAAGAAGGGGACCTTTACCGAC
GACCTTCACAAGCTGGTGGACGAATGGACGAGCAAGACAGTGGGAGCCGCCCAGCTGAAG
CCTACCCTGAACCAGCTCAAGCAGACACAGAAGCTGCAAGACATGGAGGCCCAGGCGGGG
TGGGCTGCTCCCGGCGAGGCCCGGGCTATGACCGCACCTCGGGCAGGAGTGGGAATGCCA
AGACTGCCCCCAGCACCCGGACCACTGTCCACCACCGTCATTCCCGGTGCCGCCCCGACC
CTGTCCGTGCCCACTCCAGATGGCGCCCTTGGCACCGCCCGGAGAAACCAGGTGTGGTTT
GGCCTCAGAGTCCCCCCAACGGCCTGCTGCGGCCATAGCACCCAGCCCCGCGGCGGTCAG
AGAGTGGGCTCCAAGACCGCTTCCTTTGCCGCATCAGACCCTGTTCGCTCC
Protein sequence predicted based on nucleotide sequence
MDGDGGRRDVPGTLMEPGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAA EAPGPQPPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGPEPVG TQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPGRTRRDEPEEEEDDE DDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTETWVEVAWCELQDRKLTKLERQRFK EEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRS WCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGT PEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEK VHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWV EDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIESGFFHESDVKIVAKSIRDRVALIQ WRRERIWPALQPKEQQDVGSPDKARGPPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLP PTLPTSATSLASDSTFDSGQGSTVYSDSQSSQQSVMLGSLADAAPSPAQCVCSPPVSEGP VLPQSLPSLGAYQQPTAAPGLPVGSVPAPACPPSLQQHFPDPAMSFAPVLPPPSTPMPTG PGQPAPPGQQPPPLAQPTPLPQVLAPQPVVPLQPVPPHLPPYLAPASQVGAPAQLKPLQM PQAPLQPLAQVPPQMPPIPVVPPITPLAGIDGLPPALPDLPTATVPPVPPPQYFSPAVIL PSLAAPLPPASPALPLQAVKLPHPPGAPLAMPCRTIVPNAPATIPLLAVAPPGVAALSIH SAVAQLPGQPVYPAAFPQMAPTDVPPSPHHTVQNMRATPPQPALPPQPTLPPQPVLPPQP TLPPQPVLPPQPTRPPQPVLPPQPMLPPQPVLPPQPALPVRPEPLQPHLPEQAAPAATPG SQILLGHPAPYAVDVAAQVPTVPVPPAAVLSPPLPEVLLPAAPELLPQFPSSLATVSASV QSVPTQTATLLPPANPPLPGGPGIASPCPTVQLTVEPVQEEQASQDKPPGLPQSCESYGG SDVTSGKELSDSCEGAFGGGRLEGRAARKHHRRSTRARSRQERASRPRLTILNVCNTGDK MVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILQAERETFIEQMKDVMDKAEDM LSEDTDADRGSDPGTSPPHLSTCGLGTGEESRQSQANAPVYQQNVLHTGKRWFIICPVAE HPAPEAPESSPPLPLSSLPPEASQGPCRGLTLPCLPWRRAACGAVFLSLFSAESAQSKQP PDSAPYKDQLSSKEQPSFLASQQLLSQAGPSNPPGAPPAPLAPSSPPVTALPQDGAAPAT STMPEPASGTASQAGGPGTPQGLTSELETSQPLAETHEAPLAVQPLVVGLAPCTPAPEAA STRDASAPREPLPPPAPEPSPHSGTPQPALGQPAPLLPAAVGAVSLATSQLPSPPLGPTV PPQPPSALESDGEGPPPRVGFVDSTIKSLDEKLRTLLYQEHVPTSSASAGTPVEVGDRDF TLEPLRGDQPRSEVCGGDLALPPVPKEAVSGRVQLPQPLVEKSELAPTRGAVMEQGTSSS MTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEPTDRDGGEAGESSAEP PPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDVSSPAKTVGRFSVVSTQDEWTLASPH SLRYSAPPDVYLDEAPSSPDVKLAVRRAQTASSIEVGVGEPVSSDSGDEGPRARPPVQKQ ASLPVSGSVAGDFVKKATAFLQRPSRAGSLGPETPSRVGMKVPTISVTSFHSQSSYISSD NDSELEDADIKKELQSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPT GRRRKTSKSKLKAGKLLNPLVRQLKVVASSTGHLADSSRGPPAKDPAQASVGLTADSTGL SGKAVQTQQPCSVRASLSSDICSGLASDGGGARGQGWTVYHPTSERVTYKSSSKPRARFL SGPVSVSIWSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPALHVQAQVNNSNNKKGTFTD DLHKLVDEWTSKTVGAAQLKPTLNQLKQTQKLQDMEAQAGWAAPGEARAMTAPRAGVGMP RLPPAPGPLSTTVIPGAAPTLSVPTPDGALGTARRNQVWFGLRVPPTACCGHSTQPRGGQ RVGSKTASFAASDPVRS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
