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LGALS4 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS4
Gene Name:  lectin, galactoside-binding, soluble, 4
Sequence             
Original Clone ID: FLH213120.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00074525

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 972nts         Open reading frame : 1 to 972
Reference Sequence Alignment

ATGGCCTATGTCCCCGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
GTCCAGATCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1024
Start on reference sequence 53
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS4
Symbol Nomenclature : LGALS4
SYNONYM : GAL4; L36LBP
Designation : L-36 lactose-binding protein|antigen NY-CO-27|gal-4|galectin 4|galectin-4|lactose-binding lectin 4
Full Nomenclature : lectin, galactoside-binding, soluble, 4
GENEID : 3960
HGNC : 6565
MIM : 602518
Vega : OTTHUMG00000182608
Target GenBank: BC005146

 

Reference Sequence Alignment

  

HsCD00074525      ATGGCCTATGTCCCCGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
NM_006149.3       ATGGCCTATGTCCCCGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
                  ************************************************************

HsCD00074525      CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
NM_006149.3       CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
                  ************************************************************

HsCD00074525      GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
NM_006149.3       GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
                  ************************************************************

HsCD00074525      GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
NM_006149.3       GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
                  ************************************************************

HsCD00074525      GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
NM_006149.3       GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
                  ************************************************************

HsCD00074525      TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
NM_006149.3       TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
                  ************************************************************

HsCD00074525      TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
NM_006149.3       TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
                  ************************************************************

HsCD00074525      GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
NM_006149.3       GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
                  ************************************************************

HsCD00074525      CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
NM_006149.3       CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
                  ************************************************************

HsCD00074525      ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
NM_006149.3       ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
                  ************************************************************

HsCD00074525      GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
NM_006149.3       GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
                  ************************************************************

HsCD00074525      TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
NM_006149.3       TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
                  ************************************************************

HsCD00074525      ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
NM_006149.3       ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
                  ************************************************************

HsCD00074525      AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
NM_006149.3       AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
                  ************************************************************

HsCD00074525      GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
NM_006149.3       GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
                  ************************************************************

HsCD00074525      CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
NM_006149.3       CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
                  ************************************************************

HsCD00074525      GTCCAGATCTAG
NM_006149.3       GTCCAGATCTAA
                  ***********.

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
UniProt : Q6FHZ4
UniProt : P56470
HPRD : 03949

Clone

Cloning Information : 213120
HIP Master Clone ID : 87060
Original Clone ID : FLH213120.01X

Organism


TAX_ID : 9606
Species Specific ID: 3960

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000171747

Reagents

Labome : galectin-4-antibody