Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745280
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6261nts
Open reading frame: 1 to 6261
ATGGAGACTGAAAGTGAGAGTAGCACTTTAGGGGATGACAGTGTCTTCTGGTTGGAGTCT
GAAGTTATAATCCAGGTGACTGACTGTGAAGAGGAAGAAAGGGAAGAGAAGTTCAGGAAG
ATGAAGTCCTCAGTACATTCTGAAGAAGATGATTTTGTTCCAGAGCTACATAGAAATGTA
CACCCTCGAGAGCGGCCTGATTGGGAAGAAACTCTTAGCGCAATGGCAAGAGGCGCAGAT
GTTCCAGAGATTCCTGGAGATCTTACTCTTAAGACGTGTGGCAGTACAGCCAGTATGAAG
GTTAAGCATGTGAAAAAGTTACCCTTCACTAAAGGTCACTTTCCGAAGATGGCTGAATGT
GCGCATTTCCATTATGAGAATGTTGAGTTCGGCAGCATACAGCTTTCACTTTCAGAAGAA
CAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATT
GCTTGTCAGGGAAAAAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTTGAT
AAACATCTTCATCTGTGTATTTATGACCGAAGATTTTCCCAGCTCTCAGAACTTCCCCGT
TCTGACACCCTGAAGGACAGTCCAGAGTCGGTCACTCAGATGCTTATGGCTTACCTGTCA
CGCCTTTCAGCTATCGCTGGCAACAAGATCAACTGTGGGCCCGCCCTTACCTGGATGGAG
ATTGATAATAAGGGAAATCACCTTTTGGTTCATGAGGAGTCATCCATCAACACTCCTGCT
GTCGGTGCTGCCCATGTTATCAAGAGGTACACTGCTCGGGCCCCTGACGAACTGACCTTA
GAGGTGGGAGACATTGTTTCTGTTATTGACATGCCCCCGAAAGTGTTAAGCACATGGTGG
AGAGGCAAGCACGGATTCCAGGTGGGACTCTTCCCTGGACACTGTGTTGAGTTAATTAAC
CAAAAAGTTCCCCAGTCAGTGACCAACTCAGTGCCAAAACCAGTGTCTAAAAAGCACGGC
AAGCTCATTACGTTCTTACGAACATTCATGAAGTCTCGTCCAACAAAACAGAAGCTGAAG
CAGCGGGGAATCTTGAAAGAGAGGGTGTTTGGTTGTGACCTGGGGGAACACCTTCTAAAT
TCTGGTTTTGAAGTGCCGCAGGTTCTTCAAAGCTGCACAGCATTCATTGAGAGATATGGC
ATCGTGGATGGAATCTATCGCCTTTCTGGTGTTGCCTCCAATATCCAGAGACTACGCCAT
GAATTTGACTCTGAGCACGTCCCCGACCTGACGAAAGAACCGTATGTTCAGGACATCCAT
TCTGTGGGTTCCCTATGTAAGCTGTACTTCCGGGAACTCCCAAACCCTCTGCTTACCTAC
CAGCTGTATGAGAAATTTTCTGATGCAGTTTCAGCAGCAACAGATGAAGAAAGGCTGATA
AAAATCCACGATGTCATCCAGCAGCTCCCCCCACCACACTACAGAACACTGGAGTTCCTG
ATGAGACACTTGTCTCTTCTAGCTGACTATTGTTCCATCACAAATATGCATGCAAAAAAT
CTAGCAATTGTTTGGGCTCCAAACCTGTTAAGATCAAAACAGATAGAATCTGCCTGCTTC
AGTGGAACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAGTTCATCCTG
AATCACGTTGATGTGCTGTTCAGCGGCAGAATCAGCATGGCCATGCAAGAGGGGGCAGCT
TCTCTATCAAGGCCCAAGTCCCTCCTGGTATCCTCTCCATCCACCAAACTGCTGACATTG
GAAGAGGCCCAGGCACGAACACAAGCTCAGGTCAATTCTCCAATTGTGACGGAAAATAAA
TATATCGAAGTAGGAGAAGGACCTGCTGCACTTCAGGGGAAATTTCATACCATAATTGAG
TTCCCACTTGAAAGAAAGAGGCCTCAAAATAAGATGAAAAAGTCTCCTGTGGGTAGCTGG
CGTTCCTTTTTCAACTTGGGGAAATCATCATCTGTTTCTAAACGAAAGCTGCAGCGGAAT
GAGAGTGAGCCTTCAGAGATGAAAGCCATGGCTCTGAAAGGTGGCAGGGCAGAAGGAACC
CTCCGTTCAGCTAAAAGTGAGGAGTCTCTTACATCTCTCCATGCAGTTGATGGTGATTCT
AAGCTCTTCCGACCCAGAAGACCCAGATCTTCCAGTGATGCACTGTCTGCCTCTTTTAAT
GGAGAAATGCTGGGGAACCGCTGTAACTCCTATGATAATCTGCCTCATGACAATGAGAGT
GAGGAGGAAGGAGGGCTGCTTCATATCCCAGCCCTTATGTCTCCTCATTCAGCTGAGGAT
GTTGACTTGAGCCCACCAGACATTGGAGTAGCCAGCCTGGATTTTGATCCAATGTCATTT
CAATGTAGTCCTCCTAAGGCCGAATCAGAATGTCTGGAGAGTGGTGCTTCCTTTTTAGAT
TCACCAGGATACTCCAAGGATAAACCAAGTGCCAATAAAAAGGATGCAGAAACAGGTAGT
AGCCAATGTCAGACTCCAGGAAGCACAGCAAGCTCTGAACCTGTCTCTCCTCTTCAGGAG
AAACTGAGTCCATTCTTTACCCTGGACTTGAGCCCAACTGAAGATAAATCATCTAAGCCA
TCCTCCTTTACTGAAAAGGTCGTCTATGCTTTCTCTCCGAAGATAGGACGGAAATTAAGC
AAATCACCTTCTATGAGCATATCTGAGCCAATTTCAGTGACCCTACCACCACGGGTGTCA
GAAGTCATTGGTACAGTCTCAAATACCACAGCTCAGAATGCATCATCTTCAACCTGGGAC
AAATGCGTTGAAGAAAGGGATGCCACAAATAGATCCCCCACCCAGATAGTAAAGATGAAA
ACAAATGAGACAGTTGCCCAAGAAGCATATGAATCTGAAGTCCAGCCCCTGGACCAGGTG
GCTGCTGAAGAAGTAGAATTGCCAGGGAAAGAGGATCAGTCTGTCTCAAGCAGTCAGAGT
AAGGCTGTAGCTTCTGGACAGACTCAGACAGGAGCAGTTACCCATGACCCCCCTCAGGAT
TCCGTTCCTGTCAGTTCAGTCTCTCTTATCCCACCACCACCGCCTCCGAAAAATGTTGCC
CGAATGTTGGCGCTAGCATTAGCTGAGTCCGCACAGCAAGCCTCAACTCAGTCATTGAAA
AGACCAGGGACCTCTCAGGCTGGGTATACAAATTATGGAGACATAGCGGTGGCTACAACT
GAAGATAATCTGTCCAGTTCTTACTCTGCAGTTGCTCTAGATAAGGCCTATTTCCAAACC
GATCGACCAGCAGAGCAGTTCCACCTCCAGAATAATGCACCAGGAAACTGTGACCATCCT
CTACCAGAGACAACAGCTACTGGGGATCCTACCCATTCCAACACAACTGAATCTGGGGAG
CAACATCACCAAGTAGACTTAACAGGGAATCAGCCACATCAAGCATATTTATCTGGGGAC
CCAGAAAAGGCCAGAATTACTTCAGTTCCCTTAGACTCAGAGAAGTCTGATGATCATGTA
AGTTTCCCTGAAGACCAGTCTGGGAAGAACAGTATGCCAACTGTCTCCTTCTTGGATCAG
GACCAGTCTCCACCCCGTTTCTACAGTGGAGATCAGCCTCCTTCTTATCTTGGTGCAAGT
GTGGATAAACTCCATCACCCTTTAGAATTTGCAGACAAATCTCCCACACCTCCTAATTTA
CCTAGCGATAAAATCTACCCTCCTTCTGGGTCCCCCGAAGAGAATACCAGCACAGCCACC
ATGACTTACATGACAACTACTCCAGCAACAGCCCAAATGAGCACCAAGGAAGCCAGCTGG
GATGTGGCTGAACAACCCACCACTGCTGATTTTGCTGCTGCCACACTTCAGCGCACGCAC
AGAACTAATCGTCCCCTTCCCCCTCCGCCTTCCCAGAGATCTGCAGAGCAGCCACCAGTT
GTGGGGCAGGTACAAGCAGCAACCAATATAGGATTAAATAATTCCCACAAGGTTCAAGGA
GTAGTTCCAGTTCCAGAGAGGCCACCTGAACCTCGAGCCATGGATGACCCTGCGTCTGCC
TTCATCAGTGACAGTGGTGCTGCTGCTGCTCAGTGTCCCATGGCTACAGCTGTCCAGCCA
GGCCTGCCTGAGAAAGTGCGGGACGGTGCCCGGGTCCCGCTGCTGCACCTCCGCGCCGAG
TCTGTCCCTGCGCATCCCTGTGGCTTTCCTGCACCACTGCCCCCCACCAGGATGATGGAG
AGTAAGATGATTGCTGCCATACACTCCAGCAGTGCAGATGCCACCAGCAGTTCAAATTAT
CATTCCTTTGTCACTGCTTCATCCACCTCTGTGGACGATGCATTGCCTTTACCACTTCCT
GTCCCACAACCTAAGCATGCTTCTCAGAAAACAGTTTACTCCTCCTTTGCTAGGCCCGAT
GTCACCACTGAACCCTTTGGTCCAGATAACTGTTTGCATTTCAATATGACTCCAAACTGC
CAGTACCGTCCCCAGAGTGTACCTCCCCATCACAATAAATTGGAGCAGCACCAAGTGTAT
GGTGCCAGGTCAGAGCCACCAGCCTCCATGGGTCTTCGTTATAACACATATGTGGCCCCA
GGAAGAAACGCATCTGGACACCACTCCAAGCCATGCAGCCGGGTCGAGTATGTGTCTTCT
TTGAGCTCCTCTGTCAGGAATACCTGTTACCCCGAAGACATTCCACCGTACCCTACCATC
CGGAGAGTGCAGTCTCTCCATGCTCCGCCGTCTTCCATGATTCGCTCTGTTCCCATTTCA
CGGACAGAAGTTCCCCCAGATGATGAGCCAGCCTACTGCCCAAGACCTCTGTACCAATAT
AAGCCATATCAGTCCTCCCAGGCCCGCTCAGATTATCATGTCACTCAGCTTCAGCCTTAC
TTTGAGAATGGCCGGGTCCACTACAGGTATAGCCCATATTCCAGTTCTTCTAGTTCCTAT
TACAGTCCAGATGGGGCCCTGTGTGATGTGGATGCCTATGGCACAGTCCAGTTAAGACCC
CTTCACCGCCTTCCCAATCGAGACTTTGCTTTCTACAATCCTAGGCTGCAAGGAAAGAGC
TTGTACAGTTATGCTGGTTTGGCTCCACGTCCCCGGGCCAACGTGACTGGCTATTTCTCT
CCCAACGACCATAATGTAGTCAGCATGCCTCCGGCTGCTGATGTGAAGCACACCTACACC
TCATGGGATCTTGAGGACATGGAAAAATACCGCATGCAGTCCATCCGGAGAGAGAGCCGT
GCTCGGCAGAAGGTGAAAGGGCCTGTCATGTCCCAATATGATAACATGACCCCGGCGGTG
CAGGACGACTTGGGTGGGATCTATGTCATCCATCTGCGTAGTAAATCAGATCCTGGGAAA
ACTGGACTTCTCTCAGTGGCAGAAGGAAAGGAGAGCCGCCATGCAGCCAAGGCCATCAGT
CCCGAGGGAGAGGACCGCTTCTATAGGAGGCATCCCGAGGCAGAGATGGACAGAGCCCAC
CATCACGGAGGCCATGGTAGCACGCAGCCGGAGAAGCCATCCCTGCCTCAGAAGCAGAGC
AGCCTGAGGAGCAGGAAGCTTCCTGACATGGGCTGCAGTCTTCCTGAGCACAGGGCACAC
CAAGAAGCAAGCCATAGGCAGTTCTGTGAGTCAAAGAATGGGCCCCCTTATCCCCAGGGA
GCTGGCCAGTTAGATTATGGGTCCAAAGGGATTCCAGACACTTCTGAGCCAGTCAGCTAC
CACAACTCTGGAGTAAAATATGCTGCATCCGGGCAAGAATCTTTAAGACTGAACCACAAA
GAGGTAAGGCTCTCCAAAGAGATGGAGCGACCCTGGGTTAGGCAGCCTTCTGCCCCAGAG
AAACACTCCAGAGACTGCTACAAGGAGGAAGAACACCTCACTCAGTCAATCGTCCCACCC
CCTAAACCAGAGAGGAGTCATAGCCTCAAACTCCATCATACCCAGAACGTGGAGAGGGAC
CCCAGTGTGCTGTACCAGTACCAACCACACGGCAAGCGCCAGAGCAGTGTGACTGTTGTG
TCCCAGTATGATAACCTGGAAGATTACCACTCCCTGCCTCAGCACCAGCGAGGAGTCTTT
GGAGGGGGCGGCATGGGGACGTATGTGCCCCCTGGCTTTCCCCATCCACAGAGCAGGACC
TATGCTACAGCGTTGGGTCAAGGGGCCTTCCTGCCCGCAGAGTTGTCCTTGCAGCATCCT
GAAACACAGATCCATGCAGAA
Protein sequence predicted based on nucleotide sequence
METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNV HPRERPDWEETLSAMARGADVPEIPGDLTLKTCGSTASMKVKHVKKLPFTKGHFPKMAEC AHFHYENVEFGSIQLSLSEEQNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLD KHLHLCIYDRRFSQLSELPRSDTLKDSPESVTQMLMAYLSRLSAIAGNKINCGPALTWME IDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWW RGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLK QRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRH EFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLI KIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACF SGTAAFMEVRIQSVVVEFILNHVDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTL EEAQARTQAQVNSPIVTENKYIEVGEGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSW RSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDS KLFRPRRPRSSSDALSASFNGEMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAED VDLSPPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGS SQCQTPGSTASSEPVSPLQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLS KSPSMSISEPISVTLPPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMK TNETVAQEAYESEVQPLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQD SVPVSSVSLIPPPPPPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGDIAVATT EDNLSSSYSAVALDKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTHSNTTESGE QHHQVDLTGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKNSMPTVSFLDQ DQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPEENTSTAT MTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQRSAEQPPV VGQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQCPMATAVQP GLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESKMIAAIHSSSADATSSSNY HSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPDVTTEPFGPDNCLHFNMTPNC QYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAPGRNASGHHSKPCSRVEYVSS LSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPDDEPAYCPRPLYQY KPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGALCDVDAYGTVQLRP LHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHNVVSMPPAADVKHTYT SWDLEDMEKYRMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDLGGIYVIHLRSKSDPGK TGLLSVAEGKESRHAAKAISPEGEDRFYRRHPEAEMDRAHHHGGHGSTQPEKPSLPQKQS SLRSRKLPDMGCSLPEHRAHQEASHRQFCESKNGPPYPQGAGQLDYGSKGIPDTSEPVSY HNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAPEKHSRDCYKEEEHLTQSIVPP PKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSVTVVSQYDNLEDYHSLPQHQRGVF GGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQHPETQIHAE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
