Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745325
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5691nts
Open reading frame: 1 to 5691
ATGGCTCAAGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAG
GAGCAGAAGGAGAAGGAGGAATCCAACAGGAAGCGGAGAAACCGCTCCCGTGACCGAAAG
AAGAAGGCCGATGCTGCTACCAGCTTTCTGAGAGCAGCAAGATCAGGTAACTTGGACAAA
GCTTTGGATCACCTGAGGAATGGGGTAGATATTAACACCTGTAACCAGAATGGGTTGAAT
GGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATGGTGGTTGAACTTCTGCACAAA
GAAATCATTCTAGAAACGACAACCAAGAAGGGGAACACGGCCCTGCACATCGCTGCTCTA
GCCGGGCAGGATGAGGTGGTCCGGGAGCTTGTCAACTATGGAGCCAACGTCAACGCCCAG
TCACAGAAAGGTTTTACACCCCTGTACATGGCAGCACAAGAGAACCACTTGGAAGTGGTT
AAGTTTTTACTGGAAAATGGAGCTAACCAGAATGTAGCCACAGAAGACGGCTTCACGCCT
CTGGCGGTAGCCCTGCAGCAGGGCCATGAGAACGTCGTCGCGCACCTCATCAACTACGGC
ACCAAGGGGAAGGTGCGCCTCCCGGCCCTGCACATCGCGGCCCGCAACGACGACACGCGC
ACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGACGTGCTTTCCAAGACGGGATTC
ACGCCCCTGCACATTGCGGCTCACTACGAGAACCTCAACGTGGCCCAGTTGCTCCTCAAC
AGAGGAGCCAGCGTCAATTTCACACCACAGAACGGCATCACGCCACTGCACATCGCCTCC
CGCAGGGGCAACGTGATCATGGTGCGGCTGCTGCTGGATCGGGGAGCCCAGATAGAAACC
AAGACCAAGGACGAATTGACACCTCTCCACTGTGCAGCTCGAAATGGGCACGTGCGAATC
TCAGAGATCCTGCTGGACCACGGGGCACCAATCCAAGCCAAAACCAAGAACGGCCTGTCC
CCAATTCACATGGCGGCTCAGGGCGACCACCTCGACTGTGTCCGGCTCCTGTTGCAATAC
GACGCAGAGATAGACGACATCACCCTGGACCACCTGACCCCACTCCACGTGGCTGCCCAC
TGTGGACACCACAGGGTGGCTAAGGTCCTTCTGGATAAAGGGGCCAAACCCAACTCCAGA
GCCCTGAATGGCTTTACCCCCTTACACATCGCCTGCAAAAAGAACCACGTCCGTGTCATG
GAGCTGCTGCTGAAGACGGGAGCCTCGATCGACGCGGTCACCGAGTCTGGCCTGACACCT
CTCCACGTGGCCTCCTTCATGGGGCACCTTCCCATCGTGAAGAACCTCCTGCAGCGGGGA
GCGTCGCCCAACGTCTCCAACGTGAAAGTGGAGACTCCGCTACACATGGCAGCCAGAGCC
GGGCACACGGAAGTGGCCAAATATTTACTCCAGAACAAAGCCAAAGTCAATGCCAAGGCC
AAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATCGGCCACACAAACATGGTGAAG
CTCCTGCTGGAAAATAACGCCAACCCCAACCTGGCCACCACCGCCGGGCACACCCCCCTG
CACATTGCAGCCCGTGAGGGCCATGTGGAAACAGTCCTGGCCCTTCTGGAAAAGGAAGCA
TCCCAGGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCGGCCAAGTACGGG
AAGGTGCGGGTGGCAGAGCTGCTGCTGGAGCGGGACGCACACCCGAATGCTGCCGGAAAA
AATGGCCTGACCCCCCTGCACGTGGCCGTCCATCACAACAACCTGGACATCGTCAAGCTG
CTGCTTCCCCGGGGCGGCTCCCCGCACAGCCCTGCCTGGAATGGCTACACCCCTTTGCAC
ATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGTCTGCTGCAGTATGGGGGCTCA
GCAAACGCCGAGTCGGTGCAAGGTGTGACGCCCCTTCACCTGGCCGCCCAGGAGGGCCAC
GCAGAGATGGTGGCTCTGCTGCTCTCGAAACAAGCCAATGGCAACCTGGGGAACAAGAGC
GGACTCACTCCCCTCCATCTGGTAGCACAAGAAGGCCACGTTCCAGTGGCAGATGTGCTG
ATCAAACACGGCGTCATGGTGGACGCCACCACCCGGATGGGCTACACTCCCCTCCATGTG
GCCAGTCACTATGGAAACATCAAGCTGGTGAAGTTTCTGCTGCAGCACCAGGCAGATGTC
AATGCCAAGACCAAGCTAGGATACAGCCCCCTGCACCAGGCAGCCCAGCAGGGACACACA
GACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCAAACGAGGTCAGCTCGGATGGA
ACCACACCTCTGGCCATAGCCAAGCGCTTGGGCTACATTTCTGTCACCGACGTGCTCAAG
GTCGTCACGGATGAAACCAGTTTCGTGTTAGTCAGTGATAAGCATCGAATGAGTTTCCCT
GAGACAGTTGATGAGATCCTGGATGTCTCGGAAGATGAAGGAACTGCTCATATAACTATA
ATGGGGGAAGAACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAA
GAGAAGGAGCTGCTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATC
CCCAGGATTCCCTGTGCCATGCCTGAGACAGTGGTGATCAGGTCAGAAGAGCAGGAGCAG
GCATCTAAAGAGTATGATGAGGACTCCCTCATCCCCAGCAGCCCGGCCACCGAAACCTCA
GACAACATCAGCCCGGTGGCCAGCCCGGTGCATACAGGGTTTCTGGTGAGCTTCATGGTT
GACGCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCCTGCGAGTGGTGATCCCG
CCACGGACGTGCGCAGCGCCCACCCGCATAACCTGCCGCCTGGTCAAGCCCCAGAAGCTC
AGCACGCCGCCCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGG
CCCACGGGGGCACAGTTCCTGAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCAT
GGCCGTGGGGACCGCGAGCTCGTGGTTCTGAGGAGCGAAAACGGCTCCGTGTGGAAGGAG
CACAGGAGCCGCTATGGAGAGAGCTACCTGGATCAGATCCTCAACGGGATGGACGAAGAG
CTGGGGAGCCTGGAGGAGCTAGAGAAGAAGAGGGTGTGCCGAATCATCACCACCGACTTC
CCGCTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACGACACCATCGGTCCCGAA
GGGGGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGAGAATGCC
GTCACCAAGAGAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACT
AAGCTCCTGGGCAACCAGGCCACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGG
AAGTTCCACCGCCCCATTGGGCTTCGGATCCCACTACCTCCTTCCTGGACCGACAACCCG
AGGGACAGCGGGGAGGGAGACACCACCAGCCTGCGCCTGCTTTGCAGCGTCATTGGAGGA
ACAGACCAAGCCCAGTGGGAAGACATAACAGGAACCACCAAACTTGTATATGCCAACGAG
TGCGCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGTCGGACTGTCCTCGGACT
GCTGAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCCCTACATG
GCCAAATTCGTCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTAC
TGCATGACAGATGATAAAGTGGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTG
GCCCGGAGCAGGGACATAGAGGTGTTGGAAGGAATGTCCCTGTTTGCAGAACTCTCTGGG
AACCTGGTGCCTGTGAAGAAAGCTGCCCAGCAGCGGAGCTTCCACTTCCAGTCATTTCGG
GAGAACCGTCTGGCCATGCCTGTAAAGGTGAGGGACAGCAGTCGAGAGCCGGGAGGGTCC
CTGTCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGCACATTCTCTGCCACCTG
AACATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAGGACCCCG
ACGCCCCTGGCCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGG
ACAGAGCAGGCAGAGATGAAGATGGCTGTTATCTCAGAGCACCTCGGTCTGAGCTGGGCA
GAGTTGGCCCGGGAGCTGCAGTTCAGTGTGGAAGACATCAACAGGATCCGAGTGGAAAAT
CCCAACTCCCTGTTGGAGCAGAGTGTGGCCTTGCTGAACCTCTGGGTCATCCGTGAAGGC
CAAAACGCAAACATGGAGAATCTGTACACAGCCCTGCAGAGCATTGACCGTGGCGAGATC
GTGAACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGAAGCCAGACAGGCGGCAC
ACCGACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGTTACTCCTCACTGCAGGAC
GAGCTGCTGTCCCCTGCCTCCCTGGGCTGTGCACTTTCCTCTCCGCTACGTGCAGACCAG
TACTGGAATGAGGTGGCCGTCCTAGACGCCATCCCCTTGGCGGCCACGGAGCATGACACC
ATGCTGGAGATGTCTGACATGCAAGTGTGGTCTGCGGGCCTCACGCCTTCTCTGGTCACT
GCTGAGGACTCCTCTCTGGAGTGTAGCAAGGCTGAGGACTCTGATGCCACAGGTCACGAG
TGGAAGTTGGAGGGGGCACTCTCAGAGGAACCGCGGGGCCCCGAGTTGGGCTCTCTGGAA
CTTGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTTATCGATTTGCTTGAA
CAGGAGGAAGGTCAGAGGTCAGAAGAGAAGCTGCCAGGTTCTAAGAGGCAGGATGACGCG
ACAGGTGCAGGGCAGGACTCAGAGAATGAAGTGTCTCTTGTTTCAGGCCATCAGAGGGGG
CAAGCCCGAATCACACATTCCCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGA
CTGCAGGACTGGGATGCAGACGGCTCGATTGTCTCATACCTGCAAGATGCTGCACAAGGT
TCCTGGCAAGAGGAGGTCACGCAAGGTCCACACTCATTCCAGGGAACAAGTACCATGACT
GAAGGGCTAGAGCCCGGTGGATCTCAGGAGTACGAGAAGGTCCTGGTGTCTGTAAGTGAG
CACACGTGGACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCGACAGGGACCGGAGGCAG
CAAGGCCAAGAAGAGCAAGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGTGCAGGGG
AATGAGTTTCAGAATATTCCAGGGGAACAGGTGACAGAGGAGCAATTCACGGATGAGCAG
GGCAACATTGTCACCAAGAAGATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGC
GCCGATGCCGCCCAGGAGCACGAGGAGGATCACACCTCGACACCCAACCCC
Protein sequence predicted based on nucleotide sequence
MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDK ALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAAL AGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTP LAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGF TPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIET KTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY DAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVM ELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARA GHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPL HIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGK NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGS ANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVL IKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHT DIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFP ETVDEILDVSEDEGTAHITIMGEELISFKAERRDSRDVDEEKELLDFVPKLDQVVESPAI PRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMV DARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALG PTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHRSRYGESYLDQILNGMDEE LGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLKSKLVPLVQATFPENA VTKRVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNP RDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRT AEAVNFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEV ARSRDIEVLEGMSLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAMPVKVRDSSREPGGS LSFLRKAMKYEDTQHILCHLNITMPPCAKGSGAEDRRRTPTPLALRYSILSESTPGSLSG TEQAEMKMAVISEHLGLSWAELARELQFSVEDINRIRVENPNSLLEQSVALLNLWVIREG QNANMENLYTALQSIDRGEIVNMLEGSGRQSRNLKPDRRHTDRDYSLSPSQMNGYSSLQD ELLSPASLGCALSSPLRADQYWNEVAVLDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVT AEDSSLECSKAEDSDATGHEWKLEGALSEEPRGPELGSLELVEDDTVDSDATNGLIDLLE QEEGQRSEEKLPGSKRQDDATGAGQDSENEVSLVSGHQRGQARITHSPTVSQVTERSQDR LQDWDADGSIVSYLQDAAQGSWQEEVTQGPHSFQGTSTMTEGLEPGGSQEYEKVLVSVSE HTWTEQPEAESSQADRDRRQQGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQ GNIVTKKIIRKVVRQIDLSSADAAQEHEEDHTSTPNP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
