Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745396
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3261nts
Open reading frame: 1 to 3261
ATGACAACCGAATCTGGATCCGACTCGGAATCCAAGCCTGACCAAGAGGCTGAGCCCCAG
GAAGCTGCTGGGGCTCAAGGACGAGCTGGGGCTCCCGTTCCGGAGCCTCCCAAAGAAGAG
CAACAGCAGGCTTTAGAGCAGTTCGCCGCCGCTGCAGCTCACAGCACCCCTGTACGGAGG
GAGGTCACTGACAAGGAACAGGAGTTTGCTGCCAGGGCTGCAAAACAGCTCGAATATCAG
CAATTAGAAGACGATAAACTTTCTCAGAAATCATCTAGCAGTAAACTCTCTCGCTCTCCA
TTGAAGATTGTCAAAAAGCCTAAAAGCATGCAGTGCAAAGTGATACTTCTGGATGGATCA
GAATATACCTGTGATGTAGAGAAACGCTCCAGAGGCCAAGTGCTGTTTGATAAAGTGTGT
GAACACTTGAACCTGCTAGAGAAAGACTACTTTGGGCTTACGTATCGAGATGCTGAAAAC
CAGAAGAATTGGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCTTGG
CACTTTTCATTTAATGTGAAATTTTATCCACCAGACCCTGCTCAACTATCTGAAGATATC
ACCAGGTACTACCTCTGCTTGCAGTTGCGAGATGACATCGTGTCCGGAAGGCTGCCCTGC
TCCTTTGTTACCCTGGCCTTGCTTGGCTCCTACACTGTCCAGTCAGAGCTGGGAGACTAT
GACCCAGATGAATGTGGGAGCGATTACATCAGTGAGTTCCGCTTTGCACCAAACCACACT
AAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCA
GAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAGTTATCAATGTATGGGGTAGATTTA
CATCATGCTAAGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGTCTG
TTGATATATCGCGACCGGCTTAGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATT
TCATACAAACGGAACAACTTTTACATTAAGATCAGGCCCGGAGAGTTTGAACAATTTGAA
AGCACCATCGGGTTTAAGCTGCCAAACCATCGAGCTGCCAAGCGTTTATGGAAAGTATGT
GTGGAGCATCATACATTTTTCAGACTACTCTTACCAGAAGCACCTCCCAAGAAATTCCTA
ACCTTGGGTTCTAAGTTTCGTTATAGTGGCAGGACACAAGCGCAAACGAGAAGAGCCAGT
GCGTTGATAGATCGGCCCGCACCTTACTTTGAACGCTCATCTAGCAAACGTTATACCATG
TCTCGCAGCTTGGATGGAGAGGTTGGTACTGGCCAGTACGCCACAACCAAAGGGATCTCT
CAGACAAACTTGATCACCACCGTGACTCCGGAGAAGAAGGCTGAGGAGGAGCGGGACGAG
GAAGAGGACAAACGGAGGAAGGGGGAAGAAGTCACCCCCATCTCGGCCATCCGGCACGAG
GGAAAGTCACCTGGGCTTGGCACTGACTCCTGTCCCTTGTCACCCCCATCCACCCATTGT
GCCCCCACATCTCCCACAGAGCTCCGTAGGAGGTGTAAGGAGAATGACTGCAAACTGCCA
GGTTATGAGCCGTCCAGAGCTGAGCATCTGCCTGGAGAGCCCGCCTTGGACTCTGATGGC
CCAGGGAGGCCTTACCTAGGGGATCAAGATGTGGCTTTTAGCTACAGGCAGCAAACTGGC
AAGGGGACCACCCTGTTCTCCTTCTCCTTGCAGCTCCCTGAGTCATTCCCCTCCCTCCTA
GATGATGATGGATACCTCTCCTTCCCCAACCTTTCAGAAACCAACCTCCTGCCCCAGAGC
CTGCAGCATTACCTCCCGATCCGCTCACCGTCCCTTGTGCCCTGTTTCCTCTTCATCTTT
TTCTTTCTGCTGTCTGCCTCCTTCTCAGTGCCATACGCTCTCACTCTCTCCTTCCCTCTG
GCACTCTGCCTCTGCTACCTGGAACCCAAGGCCGCCTCCTTGAGCGCCTCCCTAGACAAT
GACCCGAGTGACAGTTCAGAGGAAGAGACTGACAGTGAGCGCACGGACACCGCAGCCGAC
GGGGAAACCACCGCTACTGAGTCCGACCAGGAGGAAGATGCAGAGCTCAAGGCACAGGAG
CTAGAAAAAACCCAAGATGACCTGATGAAACATCAAACCAACATTAGCGAGCTGAAAAGA
ACCTTCTTAGAAACATCAACAGACACTGCCGTAACGAATGAATGGGAGAAGAGGCTTTCC
ACCTCACCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTC
CCAGAAGAGACTAAGCAGTCTTCCGGGGAAAAGCTCATGGATGGCTCTGAAATCTTCAGT
TTATTAGAGTCTGCGCGAAAACCAACAGAATTCATAGGAGGGGTTACTTCGACTTCTCAA
AGCTGGGTTCAGAAAATGGAAACCAAGACGGAGTCCAGCGGCATAGAGACGGAACCCACC
GTCCACCACCTCCCGCTTAGCACTGAGAAGGTGGTGCAGGAGACAGTGTTGGTGGAGGAG
AGGCGTGTGGTGCACGCGAGTGGGGATGCTTCTTACTCGGCGGGAGACAGCGGGGATGCT
GCAGCACAGCCCGCATTCACAGGCATTAAAGGGAAAGAGGGCTCTGCCTTGACGGAGGGG
GCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGGAACAGGAAGAGACAGCT
GCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGAAACTCTG
GAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACCGAAACCATCAGCTTTGGATCT
GTTTCACCGGGAGGAGTAAAGCTGGAAATTTCCACGAAGGAAGTGCCCGTAGTCCACACC
GAAACCAAAACCATCACCTATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCA
GGCGTGCTGATGAGTGCACAGACGATCACATCTGAAACCACCAGTACCACCACCACTACG
CACATCACCAAAACTGTGAAAGGGGGCATTTCAGAGACAAGGATTGAGAAGCGAATAGTC
ATCACCGGGGATGCAGACATTGACCATGACCAGGCGCTGGCTCAGGCAATTAAAGAGGCC
AAAGAGCAGCACCCTGACATGTCCGTGACCAAAGTAGTCGTCCATAAAGAGACAGAGATC
ACACCAGAAGATGGAGAGGAT
Protein sequence predicted based on nucleotide sequence
MTTESGSDSESKPDQEAEPQEAAGAQGRAGAPVPEPPKEEQQQALEQFAAAAAHSTPVRR EVTDKEQEFAARAAKQLEYQQLEDDKLSQKSSSSKLSRSPLKIVKKPKSMQCKVILLDGS EYTCDVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDAENQKNWLDPAKEIKKQVRSGAW HFSFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDY DPDECGSDYISEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDL HHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFE STIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRAS ALIDRPAPYFERSSSKRYTMSRSLDGEVGTGQYATTKGISQTNLITTVTPEKKAEEERDE EEDKRRKGEEVTPISAIRHEGKSPGLGTDSCPLSPPSTHCAPTSPTELRRRCKENDCKLP GYEPSRAEHLPGEPALDSDGPGRPYLGDQDVAFSYRQQTGKGTTLFSFSLQLPESFPSLL DDDGYLSFPNLSETNLLPQSLQHYLPIRSPSLVPCFLFIFFFLLSASFSVPYALTLSFPL ALCLCYLEPKAASLSASLDNDPSDSSEEETDSERTDTAADGETTATESDQEEDAELKAQE LEKTQDDLMKHQTNISELKRTFLETSTDTAVTNEWEKRLSTSPVRLAARQEDAPMIEPLV PEETKQSSGEKLMDGSEIFSLLESARKPTEFIGGVTSTSQSWVQKMETKTESSGIETEPT VHHLPLSTEKVVQETVLVEERRVVHASGDASYSAGDSGDAAAQPAFTGIKGKEGSALTEG AKEEGGEEVAKAVLEQEETAAASRERQEEQSAAIHISETLEQKPHFESSTVKTETISFGS VSPGGVKLEISTKEVPVVHTETKTITYESSQVDPGTDLEPGVLMSAQTITSETTSTTTTT HITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEI TPEDGED
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
