Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745856
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5403nts
Open reading frame: 1 to 5403
ATGCCTGAAAATCCTGCTACAGATAAACTCCAGGTGCTGCAGGTACTTGATCGCCTGAAA
ATGAAATTGCAGGAGAAGGGTGACACGTCGCAGAATGAGAAGTTATCTATGTTTTATGAG
ACACTAAAGAGTCCTCTCTTCAACCAGATACTCACACTTCAGCAGTCCATCAAGCAACTG
AAGGGTCAACTCAACCATATACCCTCAGATTGTTCAGCCAACTTTGATTTTTCTAGGAAA
GGTTTGTTAGTGTTCACAGATGGTTCCATTACTAATGGAAATGTCCACAGGCCCTCTAAT
AACTCGACTGTATCTGGGTTATTTCCGTGGACCCCGAAGTTGGGAAATGAAGACTTTAAC
TCAGTCATTCAACAGATGGCTCAGGGCCGGCAAATTGAATATATAGATATAGAACGGCCT
TCAACTGGAGGCCTTGGATTCAGTGTGGTGGCCCTCAGAAGTCAAAATCTCGGAAAAGTT
GATATCTTCGTGAAGGATGTCCAGCCAGGGAGTGTAGCAGACAGGGATCAAAGATTGAAG
GAAAATGATCAAATATTGGCCATTAATCACACGCCATTGGATCAGAACATTTCCCATCAG
CAAGCAATTGCATTATTACAACAAACCACTGGATCTTTGAGACTGATTGTGGCCAGGGAA
CCAGTCCACACAAAAAGCAGTACTTCTAGCAGCCTAAATGATACAACTCTGCCTGAAACA
GTTTGTTGGGGCCATGTTGAAGAGGTTGAGCTCATTAATGATGGCTCTGGCCTAGGTTTT
GGAATAGTTGGAGGAAAAACAAGTGGCGTGGTTGTGAGGACTATAGTTCCTGGAGGATTA
GCAGATCGAGATGGAAGACTCCAGACAGGGGACCACATCTTGAAGATTGGTGGCACAAAC
GTGCAGGGAATGACCAGTGAGCAAGTTGCACAAGTTCTAAGGAACTGTGGGAATTCAGTC
AGGATGCTCGTTGCTAGAGATCCAGCTGGTGACATTTCAGTCACCCCCCCTGCCCCTGCA
GCCTTACCTGTTGCCCTCCCTACTGTAGCCAGCAAGGGCCCTGGTTCTGACAGTTCTCTT
TTTGAAACTTATAATGTTGAGCTTGTGAGAAAAGATGGGCAGAGCCTTGGAATTAGAATT
GTTGGCTATGTTGGAACATCTCATACAGGGGAAGCTTCAGGGATTTATGTGAAAAGTATA
ATACCTGGCAGTGCTGCGTACCACAATGGCCACATTCAAGTGAATGACAAAATAGTTGCC
GTCGATGGCGTGAACATTCAGGGTTTTGCCAACCATGATGTTGTTGAAGTATTACGAAAT
GCAGGCCAGGTGGTACACCTAACCCTAGTTCGAAGGAAGACATCCTCATCTACTTCTCCA
CTTGAACCACCTTCAGACAGAGGAACTGTTGTAGAACCACTGAAACCACCAGCTCTCTTT
CTAACTGGAGCAGTCGAAACTGAAACTAATGTGGATGGTGAAGATGAGGAAATTAAAGAG
AGAATTGATACTCTGAAAAATGACAACATACAAGCCCTGGAAAAACTGGAAAAAGTCCCA
GACTCTCCAGAAAATGAGCTGAAATCAAGATGGGAAAACCTGTTGGGTCCTGATTATGAA
GTAATGGTTGCTACTCTGGACACACAGATTGCAGATGATGCTGAGTTACAGAAATATTCA
AAGCTGCTGCCTATTCACACTCTGAGGCTTGGTGTGGAAGTGGATTCCTTTGATGGGCAC
CATTATATTTCTTCAATTGTTTCTGGTGGTCCTGTTGATACACTGGGCCTCCTACAGCCA
GAAGATGAGCTCCTTGAGGTCAATGGCATGCAGCTTTATGGAAAATCTCGCCGAGAAGCA
GTCTCCTTTCTTAAAGAAGTGCCACCCCCTTTTACTTTGGTTTGCTGTCGGAGGTTGTTT
GATGATGAAGCTTCTGTAGATGAACCAAGGCGCACTGAAACCTCTCTTCCTGAGACAGAG
GTTGACCACAATATGGATGTCAATACTGAAGAAGATGATGATGGGGAATTAGCACTGTGG
TCCCCTGAAGTCAAGATTGTTGAACTAGTAAAAGATTGTAAAGGTTTGGGATTCAGCATT
TTGGATTACCAGGACCCTTTAGATCCTACAAGATCAGTGATTGTGATCCGCTCCCTGGTA
GCAGATGGTGTAGCAGAAAGAAGTGGGGGACTATTACCTGGAGATCGCCTGGTCAGCGTC
AATGAATACTGTTTGGACAACACCTCACTTGCTGAAGCTGTGGAAATATTGAAAGCTGTG
CCACCAGGCCTAGTACACCTTGGCATCTGTAAGCCTCTGGTGGAAGATAATGAAGAAGAA
AGTTGTTATATTCTGCATTCCAGCAGCAACGAAGACAAGACCGAATTTTCAGGAACAATC
CATGATATAAATTCCTCTTTGATACTCGAAGCACCCAAGGGATTTAGAGATGAACCATAT
TTTAAAGAAGAACTTGTGGATGAACCATTTCTAGATCTGGGAAAGTCTTTCCATTCCCAA
CAAAAAGAGATAGAGCAAAGCAAGGAGGCCTGGGAGATGCATGAATTTCTGACTCCTAGA
TTGCAGGAAATGGATGAAGAAAGAGAAATGCTTGTTGATGAAGAATATGAGTTATATCAA
GATCCCTCACCATCCATGGAGTTGTATCCCTTGTCGCACATTCAAGAGGCCACTCCTGTG
CCCTCTGTGAATGAACTTCACTTTGGTACACAGTGGTTGCATGATAATGAACCATCCGAG
TCTCAAGAGGCAAGAACCGGGAGGACTGTCTATTCCCAGGAGGCACAGCCCTATGGCTAT
TGCCCTGAAAATGTGATGAAAGAAAATTTTGTCATGGAGTCCCTACCATCTGTACCATCA
ACTGAAGGAAACAGTCAACAAGGCAGATTTGACGACCTGGAAAATCTTAATTCATTAGCA
AAAACTAGTCTGGATTTAGGCATGATCCCGAATGATGTCCAAGGTCCTAGCTTGCTCATT
GACCTTCCTGTTGTGGCTCAAAGGAGGGAGCAAGAAGATTTGCCTTTATATCAACACCAA
GCGACACGAGTTATTTCCAAGGCCTCAGCATACACAGGAATGTTGTCTTCTAGATATGCC
ACTGATACATGTGAGTTACCTGAGAGAGAAGAAGGCGAAGGAGAAGAAACTCCAAATTTT
AGCCACTGGGGTCCACCGAGAATTGTTGAGATTTTTAGAGAACCCAATGTGTCTCTTGGG
ATCAGCATTGTTGGTGGACAAACTGTTATAAAACGTCTAAAGAATGGAGAGGAGCTTAAA
GGTATATTCATCAAACAAGTTTTAGAAGACAGTCCAGCAGGGAAGACGAACGCACTTAAA
ACTGGAGATAAAATACTTGAGGTGTCTGGAGTAGATTTGCAGAATGCCTCACACAGCGAA
GCAGTTGAGGCCATTAAGAATGCAGGAAACCCTGTGGTGTTCATTGTTCAGAGTTTGTCA
TCCACTCCACGAGTCATTCCTAACGTACATAACAAGGCCAACAAAATCACCGGTAACCAG
AACCAGGACACCCAAGAAAAGAAAGAAAAGAGGCAAGGAACTGCTCCACCGCCAATGAAA
CTTCCTCCTCCTTATAAAGCTCTGACTGATGACAGTGATGAAAATGAAGAAGAAGATGCC
TTTACCGACCAAAAAATCAGACAAAGATATGCAGATCTGCCTGGAGAACTGCACATTATT
GAACTTGAAAAAGATAAGAATGGACTTGGACTCAGCCTTGCTGGTAATAAAGACCGATCA
CGCATGAGCATATTTGTGGTGGGAATTAACCCGGAAGGACCTGCTGCCGCAGATGGACGA
ATGCGTATTGGAGATGAACTCTTAGAGATAAACAATCAGATTCTGTATGGAAGAAGTCAC
CAAAATGCATCTGCCATTATTAAGACTGCCCCATCAAAGGTCAAGCTGGTTTTCATCAGA
AACGAGGATGCAGTCAATCAGATGGCCGTTACTCCCTTTCCAGTGCCATCAAGTTCTCCA
TCTTCTATTGAGGATCAGAGCGGCACCGAACCTATTAGTAGTGAGGAAGATGGCAGCGTC
GAAGTTGGTATTAAACAATTGCCTGAAAGTGAAAGCTTCAAACTGGCTGTCAGCCAGATG
AAACAGCAAAAATATCCAACAAAAGTCTCCTTCAGTTCACAAGAGATACCATTAGCACCA
GCTTCATCATACCATTCAACAGATGCAGACTTCACAGGCTATGGTGGTTTCCAGGCTCCT
CTGTCAGTGGACCCCGCAACCTGTCCCATTGTCCCTGGACAGGAAATGATTATAGAAATA
TCCAAGGGACGTTCAGGGCTTGGTCTGAGCATTGTGGGAGGAAAAGACACACCCTTGAAT
GCTATAGTTATCCATGAAGTCTATGAAGAAGGGGCAGCAGCCAGAGATGGAAGACTTTGG
GCTGGTGACCAGATATTAGAGGTTAATGGGGTTGACCTGAGGAACTCCAGCCACGAAGAA
GCCATCACAGCCCTGAGGCAGACCCCCCAGAAGGTGCGGCTGGTGGTGTATAGAGATGAG
GCACACTACCGGGATGAGGAGAACCTGGAGATTTTCCCTGTGGATCTGCAGAAGAAAGCT
GGCCGGGGCCTGGGCCTGAGCATCGTTGGGAAACGAAATGGAAGCGGAGTGTTTATTTCT
GACATCGTGAAAGGCGGAGCCGCAGACCTGGATGGGAGATTGATTCAGGGAGATCAGATC
TTATCTGTGAATGGGGAGGACATGAGAAATGCCTCACAGGAGACAGTGGCCACCATCCTC
AAGTGTGCACAGGGACTTGTGCAGCTAGAGATTGGAAGACTCCGAGCTGGTTCCTGGACC
TCCGCAAGGACGACATCACAGAACAGTCAGGGTAGTCAGCAGAGTGCACACAGTAGCTGT
CATCCCTCCTTCGCTCCTGTCATCACTGGCCTGCAAAACCTGGTTGGCACAAAAAGGGTT
TCAGATCCTTCCCAGAAAAATTCAGGCACAGATATGGAACCAAGGACTGTTGAGATAAAC
AGGGAGCTCAGTGATGCCCTTGGAATCAGTATTGCTGGAGGAAGAGGAAGTCCCTTAGGA
GATATCCCCGTATTTATTGCCATGATTCAGGCTAGCGGAGTGGCCGCACGGACACAGAAG
CTTAAAGTTGGAGATCGGATTGTCAGCATTAACGGGCAACCTTTGGATGGCCTGTCTCAC
GCGGATGTGGTTAATCTGCTGAAGAACGCCTACGGGCGCATTATCCTGCAGGTTGTAGCA
GATACCAATATAAGCGCCATAGCAGCTCAGCTTGAAAACATGTCTACAGGCTACCACCTT
GGTTCGCCCACTGCTGAACACCATCCAGAAGACACAGAGGAGCAGCTGCAGATGACGGCC
GAT
Protein sequence predicted based on nucleotide sequence
MPENPATDKLQVLQVLDRLKMKLQEKGDTSQNEKLSMFYETLKSPLFNQILTLQQSIKQL KGQLNHIPSDCSANFDFSRKGLLVFTDGSITNGNVHRPSNNSTVSGLFPWTPKLGNEDFN SVIQQMAQGRQIEYIDIERPSTGGLGFSVVALRSQNLGKVDIFVKDVQPGSVADRDQRLK ENDQILAINHTPLDQNISHQQAIALLQQTTGSLRLIVAREPVHTKSSTSSSLNDTTLPET VCWGHVEEVELINDGSGLGFGIVGGKTSGVVVRTIVPGGLADRDGRLQTGDHILKIGGTN VQGMTSEQVAQVLRNCGNSVRMLVARDPAGDISVTPPAPAALPVALPTVASKGPGSDSSL FETYNVELVRKDGQSLGIRIVGYVGTSHTGEASGIYVKSIIPGSAAYHNGHIQVNDKIVA VDGVNIQGFANHDVVEVLRNAGQVVHLTLVRRKTSSSTSPLEPPSDRGTVVEPLKPPALF LTGAVETETNVDGEDEEIKERIDTLKNDNIQALEKLEKVPDSPENELKSRWENLLGPDYE VMVATLDTQIADDAELQKYSKLLPIHTLRLGVEVDSFDGHHYISSIVSGGPVDTLGLLQP EDELLEVNGMQLYGKSRREAVSFLKEVPPPFTLVCCRRLFDDEASVDEPRRTETSLPETE VDHNMDVNTEEDDDGELALWSPEVKIVELVKDCKGLGFSILDYQDPLDPTRSVIVIRSLV ADGVAERSGGLLPGDRLVSVNEYCLDNTSLAEAVEILKAVPPGLVHLGICKPLVEDNEEE SCYILHSSSNEDKTEFSGTIHDINSSLILEAPKGFRDEPYFKEELVDEPFLDLGKSFHSQ QKEIEQSKEAWEMHEFLTPRLQEMDEEREMLVDEEYELYQDPSPSMELYPLSHIQEATPV PSVNELHFGTQWLHDNEPSESQEARTGRTVYSQEAQPYGYCPENVMKENFVMESLPSVPS TEGNSQQGRFDDLENLNSLAKTSLDLGMIPNDVQGPSLLIDLPVVAQRREQEDLPLYQHQ ATRVISKASAYTGMLSSRYATDTCELPEREEGEGEETPNFSHWGPPRIVEIFREPNVSLG ISIVGGQTVIKRLKNGEELKGIFIKQVLEDSPAGKTNALKTGDKILEVSGVDLQNASHSE AVEAIKNAGNPVVFIVQSLSSTPRVIPNVHNKANKITGNQNQDTQEKKEKRQGTAPPPMK LPPPYKALTDDSDENEEEDAFTDQKIRQRYADLPGELHIIELEKDKNGLGLSLAGNKDRS RMSIFVVGINPEGPAAADGRMRIGDELLEINNQILYGRSHQNASAIIKTAPSKVKLVFIR NEDAVNQMAVTPFPVPSSSPSSIEDQSGTEPISSEEDGSVEVGIKQLPESESFKLAVSQM KQQKYPTKVSFSSQEIPLAPASSYHSTDADFTGYGGFQAPLSVDPATCPIVPGQEMIIEI SKGRSGLGLSIVGGKDTPLNAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEE AITALRQTPQKVRLVVYRDEAHYRDEENLEIFPVDLQKKAGRGLGLSIVGKRNGSGVFIS DIVKGGAADLDGRLIQGDQILSVNGEDMRNASQETVATILKCAQGLVQLEIGRLRAGSWT SARTTSQNSQGSQQSAHSSCHPSFAPVITGLQNLVGTKRVSDPSQKNSGTDMEPRTVEIN RELSDALGISIAGGRGSPLGDIPVFIAMIQASGVAARTQKLKVGDRIVSINGQPLDGLSH ADVVNLLKNAYGRIILQVVADTNISAIAAQLENMSTGYHLGSPTAEHHPEDTEEQLQMTA D
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
