Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745888
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2736nts
Open reading frame: 1 to 2736
ATGGTAAAAGCTCCCCAGAGTGAAGAGAGGCTGGCCAGAGGAGGAAAGGAGAATAACTCA
GTTTTAGCCTGTGGAGCCCAGGCCTCTTGGAGCATCTTTGGGGCTGACGCAGCGGAGGTT
CCGGGCACACGTGGCCACTCCCAGCAGGAGGCGGCCATGCCCCACATTCCCGAGGACGAG
GAGCCCCCCGGAGAGCCACAGGCAGCCCAGAGCCCTGCCGGCCAAGGTCCTCCTGCCGCA
GGAGTATCTTGCAGTCCAACTCCCACGATTGTCCTGACTGGGGATGCCACTTCACCAGAA
GGAGAAACCGACAAAAACCTGGCCAACAGAGTTCACAGTCCCCACAAGAGGCTTTCTCAC
CGACACTTGAAGGTGTCCACTGCCTCCCTGACATCTGTGGACCCCGCGGGGCACATCATT
GACCTGGTGAATGACCAGCTGCCAGACATCAGCATCTCAGAGGAGGACAAGAAGAAAAAC
CTGGCGCTGCTGGAAGAAGCCAAGTTGGTGAGTGAGCGATTCCTGACCCGCCGTGGGAGG
AAGTCCAGGAGCAGCCCCGGAGACTCCCCATCGGCTGTTTCCCCGAACCTCAGCCCCAGC
GCTTCTCCTACATCCTCTCGGAGCAACTCACTTACAGTCCCCACCCCGCCAGGTTTGGAT
GTGTGCAGTGGCCCGCCATCCCCTCTGCCTGGAGCACCACCACAGAAGGGGGATGAGGCC
GACGTCTCTTCACCTCACCCTGGCGAGCCTAACGTCCCCAAAGGGCTAGCTGACAGGAAG
CAGAATGACCAGAGGAAAGTGTCACAGGGCAGGCTGGCTCCTCGTCCTCCTCCAGTTGAG
AAGTCCAAAGAGATTGCAATAGAACAAAAGGAAAACTTCGATCCCCTCCAGTACCCCGAG
ACAACACCCAAAGGCCTAGCTCCTGTTACAAACAGCAGTGGGAAAATGGCCCTGAACAGC
CCTCAGCCTGGCCCCGTGGAGAGCGAGCTGGGGAAGCAGCTCTTGAAAACGGGCTGGGAG
GGCAGCCCTCTGCCGAGAAGTCCAACCCAGGATGCGGCAGGAGTGGGTCCCCCAGCCTCC
CAGGGGAGAGGCCCAGCTGGAGAGCCGATGGGGCCCGAGGCTGGCTCCAAAGCTGAGCTT
CCACCCACTGTGTCCCGGCCCCCGCTGCTGCGAGGGCTCTCCTGGGACAGTGGCCCTGAA
GAACCTGGCCCCCGGCTGCAGAAAGTGCTTGCCAAGCTGCCACTAGCAGAGGAAGAAAAG
CGTTTTGCAGGCAAGGCCGGCGGCAAGCTGGCCAAGGCCCCTGGTCTGAAAGACTTTCAG
ATACAAGTGCAGCCCGTGCGGATGCAGAAACTGACCAAGCTCCGAGAGGAGCACATCCTG
ATGAGAAATCAGAACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTGAGCAG
GAAAAAGGGCTTCCTTCTGAACTCTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGTGGC
TTAGATGTCATGCCTAATATTTCTGATGTGCTGCTGCGCAAACTCCGGGTCCACAGGAGT
CTCCCTGGAAGTGCCCCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTTTGTGCAACTG
TCCTTGGCCTTTAGAAATGACAGCTACACTCTGGAATCTAGAATTAACCAGGCTGAAAGG
GAACGCAACCTGACAGAGGAGAACACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCATT
ACGTCCTCAGCTTCACTCTGGCACCACTGTGAGCACCGGGAAACCTACCAGAAGTTGCTG
GAGGACATCGCTGTCCTGCACCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGGTGGTA
GGCGCCGTCCGCCAGGAAAAGCGCATGTCGAAAGCAACGGAAGTGATGATGCAGTATGTG
GAGAATCTAAAGAGGACGTATGAGAAGGACCATGCGGAGCTCATGGAGTTTAAAAAGCTT
GCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGAAGATGGGGTCCCTCGCACGGCA
CGGTCCATGTCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTCAGCGTTGCTGTG
GTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTCCC
TCCTTACCAGCCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTACCTGTCACTTCAGCA
CTGCCTGCACTTTTGGAAAATGGAAAGACAAATGGGGACCCAGATTGTGAAGCCTCTGCT
CCTGCGCTGACCCTGAGCTGCCTGGAGGAGCTTAGTCAGGAAACCAAGGCCAGGATGGAG
GAAGAAGCCTACAGCAAGGGATTCCAAGAAGGTCTAAAGAAGACCAAAGAACTTCAAGAC
CTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCCTGAGGAACCTGAAGAGGTAGAA
GAAACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAATTGGTCCATTTC
TTACAAGTCATGTATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTGCA
GTGATGCTGGTCTTGACTGTTGTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAG
CAGGCTGATGGGCCCCTTGGAAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCTGGTGG
AGCTCAGGACTCCAGCATGAGCAGCCTACAGAGCAG
Protein sequence predicted based on nucleotide sequence
MVKAPQSEERLARGGKENNSVLACGAQASWSIFGADAAEVPGTRGHSQQEAAMPHIPEDE EPPGEPQAAQSPAGQGPPAAGVSCSPTPTIVLTGDATSPEGETDKNLANRVHSPHKRLSH RHLKVSTASLTSVDPAGHIIDLVNDQLPDISISEEDKKKNLALLEEAKLVSERFLTRRGR KSRSSPGDSPSAVSPNLSPSASPTSSRSNSLTVPTPPGLDVCSGPPSPLPGAPPQKGDEA DVSSPHPGEPNVPKGLADRKQNDQRKVSQGRLAPRPPPVEKSKEIAIEQKENFDPLQYPE TTPKGLAPVTNSSGKMALNSPQPGPVESELGKQLLKTGWEGSPLPRSPTQDAAGVGPPAS QGRGPAGEPMGPEAGSKAELPPTVSRPPLLRGLSWDSGPEEPGPRLQKVLAKLPLAEEEK RFAGKAGGKLAKAPGLKDFQIQVQPVRMQKLTKLREEHILMRNQNLVGLKLPDLSEAAEQ EKGLPSELSPAIEEEESKSGLDVMPNISDVLLRKLRVHRSLPGSAPPLTEKEVENVFVQL SLAFRNDSYTLESRINQAERERNLTEENTEKELENFKASITSSASLWHHCEHRETYQKLL EDIAVLHRLAARLSSRAEVVGAVRQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKL ANQNSSRSCGPSEDGVPRTARSMSLTLGKNMPRRRVSVAVVPKFNALNLPGQTPSSSSIP SLPALSESPNGKGSLPVTSALPALLENGKTNGDPDCEASAPALTLSCLEELSQETKARME EEAYSKGFQEGLKKTKELQDLKEEEEEQKSESPEEPEEVEETEEEEKGPRSSKLEELVHF LQVMYPKLCQHWQVIWMMAAVMLVLTVVLGLYNSYNSCAEQADGPLGRSTCSAAQRDSWW SSGLQHEQPTEQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
