Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746640
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5595nts
Open reading frame: 1 to 5595
ATGTCCCAGAAATCCTGGATAGAAAGCACTTTAACCAAGAGGGAATGTGTGTATATTATA
CCAAGTTCCAAGGACCCTCACAGATGCCTTCCAGGATGTCAAATTTGTCAGCAACTCGTC
AGGTGTTTTTGTGGTCGCTTGGTCAAGCAACATGCTTGTTTTACTGCAAGTCTTGCCATG
AAATACTCAGATGTGAAATTGGGCGACCATTTTAATCAGGCAATAGAAGAATGGTCCGTG
GAAAAGCATACAGAACAGAGCCCAACGGATGCTTATGGAGTGATAAATTTTCAAGGGGGT
TCTCATTCCTACAGAGCTAAGTATGTGAGGCTATCATATGACACCAAACCTGAAGTCATT
CTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATCTCCGTACAT
GGGGGCATGCAGAAATTTGAGCTTCACCCACGAATCAAGCAGTTGCTTGGCAAAGGTCTT
ATTAAAGCTGCAGTTACAACTGGAGCCTGGATTTTAACTGGCGGCGTAAACACAGGTGTG
GCAAAACATGTTGGAGATGCCCTCAAAGAACATGCTTCCAGATCATCTCGAAAGATTTGC
ACTATCGGAATAGCTCCATGGGGAGTGATTGAAAACAGAAATGATCTTGTTGGGAGAGAT
GTGGTTGCTCCTTATCAAACCTTATTGAACCCCCTGAGCAAATTGAATGTTTTGAATAAT
CTCCATTCCCATTTCATATTGGTGGATGATGGCACTGTTGGAAAGTATGGCGCCGAAGTC
AGACTGAGAAGAGAACTTGAAAAAACTATTAATCAGCAAAGAATTCATGCCAGGATTGGC
CAGGGTGTCCCCGTGGTGGCACTTATATTTGAGGGTGGGCCAAATGTTATCCTCACAGTT
CTTGAATACCTTCAGGAAAGCCCCCCTGTTCCAGTGGTTGTGTGTGAAGGAACAGGCAGA
GCTGCCGATCTCCTCGCGTATATTCATAAACAAACAGAAGAAGGAGGGAATCTTCCTGAT
GCAGCAGAGCCCGATATTATTTCCACTATTAAAAAAACATTTAACTTTGGCCAGAATGAA
GCACTTCATTTATTTCAAACACTGATGGAGTGCATGAAAAGAAAGGAGCTTATCACTGTT
TTCCATATTGGGTCAGATGAACATCAAGATATAGATGTAGCCATACTTACCGCACTGCTA
AAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGATAGAGTT
GACATTGCCAAAAATCATGTATTTGTTTATGGCCAGCAGTGGCTGGTTGGATCCTTGGAA
CAAGCTATGCTTGATGCTCTTGTAATGGATCGGGTTGCATTTGTAAAACTTCTTATTGAA
AATGGAGTGAGCATGCATAAATTCCTTACCATTCCCAGGCTGGAAGAACTTTACAACACT
AAACAAGGTCCAACTAATCCAATGCTGTTTCATCTTGTTCGAGACGTCAAACAGGGAAAT
CTTCCTCCAGGATATAAGATCACTCTGATTGATATAGGACTTGTTATTGAATATCTCATG
GGAGGAACCTACAGATGCACCTATACTAGGAAACGTTTTCGATTAATATATAATAGTCTT
GGTGGAAATAATCGGAGGTCTGGCCGAAATACCTCCAGCAGCACTCCTCAGTTGCGAAAG
AGTCATGAATCTTTTGGCAATAGGGCAGATAAAAAGGAAAAAATGAGGCATAACCATTTC
ATTAAGACAGCACAGCCTTACCGACCAAAGATTGATACAGTTATGGAAGAAGGAAAGAAG
AAAAGAACCAAAGATGAAATTGTAGACATTGATGATCCAGAAACCAAGCGCTTTCCTTAT
CCACTTAATGAACTTTTAATTTGGGCTTGCCTTATGAAGAGGCAGGTCATGGCCCGTTTT
TTATGGCAACATGGTGAAGAATCCATGGCTAAAGCATTAGTTGCCTGTAAGATCTATCGT
TCAATGGCATATGAAGCAAAGCAGAGTGACCTGGTAGATGATACTTCCGAAGAACTAAAA
CAGTATTCCAATGATTTTGGTCAGTTGGCCGTTGAATTATTGGAACAGTCCTTCAGACAA
GATGAAACCATGGCTATGAAACTGCTCACTTATGAACTGAAGAACTGGAGTAATTCAACC
TGCCTTAAGCTAGCAGTTTCCTCAAGACTTAGACCTTTTGTAGCCCACACCTGTACACAA
ATGTTGTTATCTGATATGTGGATGGGAAGGCTCAATATGAGGAAAAATTCCTGGTACAAG
GTCATACTAAGCATTTTGGTTCCCCCTGCCATATTGCTGTTAGAGTATAAAACTAAGGCT
GAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAGATGACAATGGATGACAGCGAA
AACAACTTTCAGAACATCACAGAAGAGATCCCCATGGAAGTGTTTAAAGAAGTACGGATT
TTGGATAGTAATGAAGGAAAGAATGAGATGGAGATACAAATGAAATCTAAAAAGCTGCCA
ATTACGCGAAAGTTTTATGCCTTTTATCATGCACCAATTGTAAAATTCTGGTTTAACACC
TTGGCATATTTAGGATTTCTGATGCTTTATACATTTGTGGTTCTTGTACAAATGGAACAG
TTACCCTCAGTTCAAGAATGGATTGTTATTGCTTATATTTTTACTTATGCCATTGAGAAA
GTCCGGGAGATCTTTATGTCTGAAGCTGGGAAAGTAAACCAGAAGATTAAAGTATGGTTT
AGTGATTACTTCAACATCAGTGATACAATTGCCATAATTTCTTTCTTCATTGGATTTGGC
CTAAGATTTGGAGCCAAATGGAACTTTGCAAATGCATATGATAATCATGTTTTTGTGGCT
GGAAGATTAATTTACTGTCTTAACATAATATTTTGGTATGTGCGTTTGCTGGATTTTCTA
GCTGTAAATCAACAGGCAGGCCCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATG
TTCTACATTGTGGTGATTATGGCTCTTGTATTACTTAGTTTTGGTGTTCCTAGAAAGGCA
ATACTTTATCCCCATGAAGCACCATCTTGGACTCTTGCTAAAGATATAGTTTTTCACCCA
TACTGGATGATTTTTGGTGAAGTTTATGCATACGAAATTGATGTGTGTGCAAATGATTCT
GTTATCCCTCAAATCTGTGGTCCTGGGACGTGGTTGACTCCATTTCTTCAAGCAGTCTAC
CTCTTTGTACAGTATATCATTATGGTTAATCTTCTTATTGCATTTTTCAACAACGTGTAT
TTACAAGTGAAGGCAATTTCCAATATCGTATGGAAGTACCAGCGTTATCATTTTATTATG
GCTTATCATGAGAAACCAGTTCTGCCTCCTCCACTTATCATTCTTAGCCATATAGTTTCT
CTGTTTTGCTGCATATGTAAGAGAAGAAAGAAAGATAAGACTTCCGATGGACCAAAACTT
TTCTTAACAGAAGAAGATCAAAAGAAGCTTCATGATTTTGAAGAGCAGTGTGTTGAAATG
TATTTCAACGAAAAAGATGACAAATTTCATTCCGGGAGTGAAGAGAGAATTCGTGTCACT
TTTGAAAGAGTGGAACAGATGTGCATTCAGATTAAAGAAGTTGGAGATCGTGTCAACTAC
ATAAAAAGATCATTACAATCATTAGATTCTCAAATTGGCCATTTGCAAGATCTTTCAGCC
CTGACGGTAGATACATTAAAAACACTCACTGCCCAGAAAGCGTCGGAAGCTAGCAAAGTT
CATAATGAAATCACAAGAGAACTCAGCATTTCCAAACACTTGGCTCAAAACCTTATTGAT
GATGGTCCCGTACGCCCTTCAGTATGGAAAAAGCATGGTGTTGTAAATACACTTAGCTCC
TCTCTGCCCCAAGGTGATCTTGAAAGTAATAATCCTTTTCATTGTAATATTTTAATGAAA
GATGACAAAGATCCCCAGTGTAATATATTTGGTCAAGACTTACCAGCAGTACCCCAGAGA
AAAGAATTTAATTTTCCAGAGGCTGGTTCCTCTTCTGGTGCCTTATTCCCAAGTGCTGTT
TCCCCTCCAGAACTGCGACAGAGACTACATGGGGTAGAACTCTTAAAAATATTTAATAAA
AATCAAAAACTAGGCAGTTCATCTACTAGCATACCACATCTGTCATCCCCACCAACCAAA
TTTTTTGTTAGTACACCATCTCAGCCAAGTTGCAAAAGCCACTTGGAAACTGGAACCAAA
GATCAAGAAACTGTTTGCTCTAAAGCTACAGAAGGAGATAATACAGAATTTGGAGCATTT
GTAGGACACAGGGATAGCATGGATTTACAGAGATTTAAAGAAACATCAAACAAGATAAAA
ATACTATCCAATAACAACACTTCTGAAAACACTTTGAAACGAGTGAGTTCTCTTGCTGGA
TTTACTGACTGTCACAGAACTTCCATTCCTGTTCATTCAAAACAAGCAGAAAAAATCAGT
AGAAGGCCATCTACCGAAGACACTCATGAAGTAGATTCTAAAGCAGCTTTAATACCGGAT
TGGTTGCAAGATAGACCATCAAACAGAGAAATGCCATCTGAAGAAGGAACATTAAATGGC
CTCACTTCTCCATTTAAGCCAGCTATGGATACAAATTACTATTATTCAGCTGTGGAAAGA
AATAACTTGATGAGGTTATCCCAGAGCATTCCATTTACACCTGTGCCACCAAGAGGGGAG
CCTGTCACAGTGTATCGGTTGGAAGAGAGTTCTCCCAACATACTAAATAACAGCATGTCT
TCTTGGTCACAACTAGGCCTCTGTGCCAAAATAGAGTTTTTAAGCAAAGAGGAGATGGGA
GGAGGTTTACGAAGAGCTGTCAAAGTACAGTGTACCTGGTCAGAACATGATATCCTCAAA
TCAGGGCATCTTTATATTATCAAATCTTTTCTTCCAGAGGTGGTTAATACATGGTCAAGT
ATTTACAAAGAAGATACAGTCCTGCATCTTTGTCTGAGAGAAATTCAACAACAGAGGGCA
GCACAAAAGCTTACGTTTGCCTTTAATCAAATGAAACCCAAATCCATACCATATTCTCCA
AGGTTCCTTGAAGTTTTCCTGCTGTATTGCCATTCAGCAGGACAGTGGTTTGCTGTGGAA
GAATGTATGACTGGAGAGTTTAGGAAATACAACAATAATAATGGAGATGAGATTATTCCA
ACTAATACTCTGGAAGAGATCATGCTAGCCTTTAGCCACTGGACTTACGAATATACAAGA
GGGGAGTTACTGGTACTTGATTTGCAAGGTGTTGGTGAAAATTTGACTGACCCCTCTGTG
ATAAAAGCAGAAGAAAAGAGATCATGTGATATGGTTTTTGGCCCAGCAAATCTAGGAGAA
GATGCAATTAAAAACTTCAGAGCAAAACATCACTGTAATTCTTGCTGTAGAAAGCTTAAA
CTTCCAGATCTGAAGAGGAATGACTATACGCCTGATAAAATTATATTTCCTCAGGATGAG
CCTTCAGATTTGAATCTTCAACCTGGAAATTCCACCAAAGAATCAGAATCAACTAATTCT
GTTCGACTGATGTTA
Protein sequence predicted based on nucleotide sequence
MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAM KYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVI LQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGV AKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNN LHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTV LEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNE ALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRV DIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNT KQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSL GGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKK KRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYR SMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNST CLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKA EMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLP ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEK VREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVA GRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKA ILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVY LFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVS LFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVT FERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV HNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMK DDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNK NQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAF VGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKIS RRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVER NNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMG GGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRA AQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIP TNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGE DAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNS VRLML
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
