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ACSM1 (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ACSM1
Gene Name:  acyl-CoA synthetase medium-chain family member 1
Sequence             
Original Clone ID: HR6714A-36-564-15.1
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Northeast Structural Genomics Consortium
Rutgers University
PSI:Biology
HsCD00751571

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 1593nts         Open reading frame : 1 to 1593

ATGGGAGCCCCAAGATGGAATGACTATGAAGTACCGGAGGAATTTAACTTTGCAAGTTAT
GTACTGGACTACTGGGCTCAAAAGGAGAAGGAGGGCAAGAGAGGTCCAAATCCAGCTTTT
TGGTGGGTGAATGGCCAAGGGGATGAAGTAAAGTGGAGCTTCAGAGAGATGGGAGACCTA
ACCCGCCGTGTAGCCAACGTCTTCACACAGACCTGTGGCCTACAACAGGGAGACCATCTG
GCCTTGATGCTGCCTCGAGTTCCTGAGTGGTGGCTGGTGGCTGTGGGCTGCATGCGAACA
GGGATCATCTTCATTCCTGCGACCATCCTGTTGAAGGCCAAAGACATTCTCTATCGACTA
CAGTTGTCTAAAGCCAAGGGCATTGTGACCATAGATGCCCTTGCCTCAGAGGTGGACTCC
ATAGCTTCTCAGTGCCCCTCTCTGAAAACCAAGCTCCTGGTGTCTGATCACAGCCGTGAA
GGGTGGCTGGACTTCCGATCGCTGGTTAAATCAGCATCCCCAGAACACACCTGTGTTAAG
TCAAAGACCTTGGACCCAATGGTCATCTTCTTCACCAGTGGGACCACAGGCTTCCCCAAG
ATGGCAAAACACTCCCATGGGTTGGCCTTACAACCCTCCTTCCCAGGAAGTAGGAAATTA
CGGAGCCTGAAGACATCTGATGTCTCCTGGTGCCTGTCGGACTCAGGATGGATTGTGGCT
ACCATTTGGACCCTGGTAGAACCATGGACAGCGGGTTGTACAGTCTTTATCCACCATCTG
CCACAGTTTGACACCAAGGTCATCATACAGACATTGTTGAAATACCCCATTAACCACTTT
TGGGGGGTATCATCTATATATCGAATGATTCTGCAGCAGGATTTCACCAGCATCAGGTTC
CCTGCCCTGGAGCACTGCTATACTGGCGGGGAGGTCGTGTTGCCCAAGGATCAGGAGGAG
TGGAAAAGACGGACGGGCCTTCTGCTCTACGAGAACTATGGGCAGTCGGAAACGGGACTA
ATTTGTGCCACCTACTGGGGAATGAAGATCAAGCCGGGTTTCATGGGGAAGGCCACTCCA
CCCTACGACGTCCAGGTCATTGATGACAAGGGCAGCATCCTGCCACCTAACACAGAAGGA
AACATTGGCATCAGAATCAAACCTGTCAGGCCTGTGAGCCTCTTCATGTGCTATGAGGGT
GACCCAGAGAAGACAGCTAAAGTGGAATGTGGGGACTTCTACAACACTGGGGACAGAGGT
AAGATGGATGAAGAGGGCTACATTTGTTTCCTGGGGAGGAGTGATGACATCATTAATGCC
TCTGGGTATCGCATCGGGCCTGCAGAGGTTGAAAGCGCTTTGGTGGAGCACCCAGCGGTG
GCGGAGTCAGCCGTGGTGGGCAGCCCAGACCCGATTCGAGGGGAGGTGGTGAAGGCCTTT
ATTGTCCTGACCCCACAGTTCCTGTCCCATGACAAGGATCAGCTGACCAAGGAACTGCAG
CAGCATGTCAAGTCAGTGACAGCCCCATACAAGTACCCAAGGAAGGTGGAGTTTGTCTCA
GAGCTGCCAAAAACCATCACTGGCAAGATTTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: 
Mutations:
None
Discrepancy:
a1207g;t1290a,F429L;

c1386t,E461D;a1565g,L521R;

g1575a
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR6714A
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism

Gene

Target GenBank: None

 

Protein

Structural Annotation Wiki : HR6714A
UniProt : Q08AH1

Clone

Cloning Information : 628576
Original Clone ID : HR6714A-36-564-15.1

Experimental

TargetTrack - Experimental data : HR6714A

Organism


Species Specific ID: 116285