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IFNGR2 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  IFNGR2
Gene Name:  interferon gamma receptor 2 (interferon gamma transducer 1)
Sequence             
Original Clone ID: FLH251679.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00075278

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1014nts         Open reading frame : 1 to 1014
Reference Sequence Alignment

ATGCGACCGACGCTGCTGTGGTCGCTGCTGCTGCTGCTCGGAGTCTTCGCCGCCGCCGCC
GCGGCCCCGCCAGACCCTCTTTCCCAGCTGCCCGCTCCTCAGCACCCGAAGATTCGCCTG
TACAACGCAGAGCAGGTCCTGAGTTGGGAGCCAGTGGCCCTGAGCAATAGCACGAGGCCT
GTTGTCTACCAAGTGCAGTTTAAATACACCGACAGTAAATGGTTCACGGCCGACATCATG
TCCATAGGGGTGAATTGTACACAGATCACAGCAACAGAGTGTGACTTCACTGCCGCCAGT
CCCTCAGCAGGCTTCCCAATGGATTTCAATGTCACTCTACGCCTTCGAGCTGAGCTGGGA
GCACTCCATTCTGCCTGGGTGACAATGCCTTGGTTTCAACACTATCGGAATGTGACTGTC
GGGCCTCCAGAAAACATTGAGGTGACCCCAGGAGAAGGCTCCCTCATCATCAGGTTCTCC
TCTCCCTTTGACATCGCTGATACCTCCACGGCCTTTTTTTGTTATTATGTCCATTACTGG
GAAAAAGGAGGAATCCAACAGGTCAAAGGCCCTTTCAGAAGCAACTCCATTTCATTGGAT
AACTTAAAACCCTCCAGAGTGTACTGTTTACAAGTCCAGGCACAACTGCTTTGGAACAAA
AGTAACATCTTTAGAGTCGGGCATTTAAGCAACATATCTTGCTACGAAACAATGGCAGAT
GCCTCCACTGAGCTTCAGCAAGTCATCCTGATCTCCGTGGGAACATTTTCGTTGCTGTCG
GTGCTGGCAGGAGCCTGTTTCTTCCTGGTCCTGAAATATAGAGGCCTGATTAAATACTGG
TTTCACACTCCACCAAGCATCCCATTACAGATAGAAGAGTATTTAAAAGACCCAACTCAG
CCCATCTTAGAGGCCTTGGACAAGGACAGCTCACCAAAGGATGACGTCTGGGACTCTGTG
TCCATTATCTCGTTTCCGGAAAAGGAGCAAGAAGATGTTCTCCAAACGCTTTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1139
Start on reference sequence 126
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IFNGR2
Symbol Nomenclature : IFNGR2
SYNONYM : AF-1; IFGR2; IFNGT1
Designation : IFN-gamma receptor 2|IFN-gamma-R2|interferon gamma receptor 2|interferon gamma receptor accessory factor 1|interferon gamma receptor accessory factor-1|interferon gamma receptor beta chain|interferon gamma transducer 1
Full Nomenclature : interferon gamma receptor 2 (interferon gamma transducer 1)
GENEID : 3460
HGNC : 5440
MIM : 147569
Vega : OTTHUMG00000065188
Target GenBank: BC003624

 

Reference Sequence Alignment

  

HsCD00075278      ATGCGACCGACGCTGCTGTGGTCGCTGCTGCTGCTGCTCGGAGTCTTCGCCGCCGCCGCC
NM_005534.3       ATGCGACCGACGCTGCTGTGGTCGCTGCTGCTGCTGCTCGGAGTCTTCGCCGCCGCCGCC
                  ************************************************************

HsCD00075278      GCGGCCCCGCCAGACCCTCTTTCCCAGCTGCCCGCTCCTCAGCACCCGAAGATTCGCCTG
NM_005534.3       GCGGCCCCGCCAGACCCTCTTTCCCAGCTGCCCGCTCCTCAGCACCCGAAGATTCGCCTG
                  ************************************************************

HsCD00075278      TACAACGCAGAGCAGGTCCTGAGTTGGGAGCCAGTGGCCCTGAGCAATAGCACGAGGCCT
NM_005534.3       TACAACGCAGAGCAGGTCCTGAGTTGGGAGCCAGTGGCCCTGAGCAATAGCACGAGGCCT
                  ************************************************************

HsCD00075278      GTTGTCTACCAAGTGCAGTTTAAATACACCGACAGTAAATGGTTCACGGCCGACATCATG
NM_005534.3       GTTGTCTACCAAGTGCAGTTTAAATACACCGACAGTAAATGGTTCACGGCCGACATCATG
                  ************************************************************

HsCD00075278      TCCATAGGGGTGAATTGTACACAGATCACAGCAACAGAGTGTGACTTCACTGCCGCCAGT
NM_005534.3       TCCATAGGGGTGAATTGTACACAGATCACAGCAACAGAGTGTGACTTCACTGCCGCCAGT
                  ************************************************************

HsCD00075278      CCCTCAGCAGGCTTCCCAATGGATTTCAATGTCACTCTACGCCTTCGAGCTGAGCTGGGA
NM_005534.3       CCCTCAGCAGGCTTCCCAATGGATTTCAATGTCACTCTACGCCTTCGAGCTGAGCTGGGA
                  ************************************************************

HsCD00075278      GCACTCCATTCTGCCTGGGTGACAATGCCTTGGTTTCAACACTATCGGAATGTGACTGTC
NM_005534.3       GCACTCCATTCTGCCTGGGTGACAATGCCTTGGTTTCAACACTATCGGAATGTGACTGTC
                  ************************************************************

HsCD00075278      GGGCCTCCAGAAAACATTGAGGTGACCCCAGGAGAAGGCTCCCTCATCATCAGGTTCTCC
NM_005534.3       GGGCCTCCAGAAAACATTGAGGTGACCCCAGGAGAAGGCTCCCTCATCATCAGGTTCTCC
                  ************************************************************

HsCD00075278      TCTCCCTTTGACATCGCTGATACCTCCACGGCCTTTTTTTGTTATTATGTCCATTACTGG
NM_005534.3       TCTCCCTTTGACATCGCTGATACCTCCACGGCCTTTTTTTGTTATTATGTCCATTACTGG
                  ************************************************************

HsCD00075278      GAAAAAGGAGGAATCCAACAGGTCAAAGGCCCTTTCAGAAGCAACTCCATTTCATTGGAT
NM_005534.3       GAAAAAGGAGGAATCCAACAGGTCAAAGGCCCTTTCAGAAGCAACTCCATTTCATTGGAT
                  ************************************************************

HsCD00075278      AACTTAAAACCCTCCAGAGTGTACTGTTTACAAGTCCAGGCACAACTGCTTTGGAACAAA
NM_005534.3       AACTTAAAACCCTCCAGAGTGTACTGTTTACAAGTCCAGGCACAACTGCTTTGGAACAAA
                  ************************************************************

HsCD00075278      AGTAACATCTTTAGAGTCGGGCATTTAAGCAACATATCTTGCTACGAAACAATGGCAGAT
NM_005534.3       AGTAACATCTTTAGAGTCGGGCATTTAAGCAACATATCTTGCTACGAAACAATGGCAGAT
                  ************************************************************

HsCD00075278      GCCTCCACTGAGCTTCAGCAAGTCATCCTGATCTCCGTGGGAACATTTTCGTTGCTGTCG
NM_005534.3       GCCTCCACTGAGCTTCAGCAAGTCATCCTGATCTCCGTGGGAACATTTTCGTTGCTGTCG
                  ************************************************************

HsCD00075278      GTGCTGGCAGGAGCCTGTTTCTTCCTGGTCCTGAAATATAGAGGCCTGATTAAATACTGG
NM_005534.3       GTGCTGGCAGGAGCCTGTTTCTTCCTGGTCCTGAAATATAGAGGCCTGATTAAATACTGG
                  ************************************************************

HsCD00075278      TTTCACACTCCACCAAGCATCCCATTACAGATAGAAGAGTATTTAAAAGACCCAACTCAG
NM_005534.3       TTTCACACTCCACCAAGCATCCCATTACAGATAGAAGAGTATTTAAAAGACCCAACTCAG
                  ************************************************************

HsCD00075278      CCCATCTTAGAGGCCTTGGACAAGGACAGCTCACCAAAGGATGACGTCTGGGACTCTGTG
NM_005534.3       CCCATCTTAGAGGCCTTGGACAAGGACAGCTCACCAAAGGATGACGTCTGGGACTCTGTG
                  ************************************************************

HsCD00075278      TCCATTATCTCGTTTCCGGAAAAGGAGCAAGAAGATGTTCTCCAAACGCTTTGA
NM_005534.3       TCCATTATCTCGTTTCCGGAAAAGGAGCAAGAAGATGTTCTCCAAACGCTTTGA
                  ******************************************************

Pathways

Panther : Interferon-gamma signaling pathway
Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Interferon Signaling
Reactome : Interferon gamma signaling
Reactome : Regulation of IFNG signaling
WikiPathway : Type II interferon signaling (IFNG)

Protein

SMART domain : FN3 : Fibronectin type 3 domain
UniProt : P38484
UniProt : A8K881
HPRD : 00956

Clone

Cloning Information : 251679
HIP Master Clone ID : 92380
Original Clone ID : FLH251679.01X

Organism


TAX_ID : 9606
Species Specific ID: 3460

Genome

Chromosome : 21
Map Location : 21q22.11
Ensembl : ENSG00000159128

Reagents

Labome : IFNGR2-antibody