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DNAJA1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DNAJA1
Gene Name:  DnaJ (Hsp40) homolog, subfamily A, member 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH169265.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23359685
Title: Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome
Authors: HIP
HsCD00075966

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1194nts         Open reading frame : 1 to 1194
Reference Sequence Alignment

ATGGTGAAAGAAACAACTTACTACGATGTTTTGGGGGTCAAACCCAATGCTACTCAGGAA
GAATTGAAAAAGGCTTATAGGAAACTGGCCTTGAAGTACCATCCTGATAAGAACCCAAAT
GAAGGAGAGAAGTTTAAACAGATTTCTCAAGCTTACGAAGTTCTCTCTGATGCAAAGAAA
AGGGAATTATATGACAAAGGAGGAGAACAGGCAATTAAAGAGGGTGGAGCAGGTGGCGGT
TTTGGCTCCCCCATGGACATCTTTGATATGTTTTTTGGAGGAGGAGGAAGGATGCAGAGA
GAAAGGAGAGGTAAAAATGTTGTACATCAGCTCTCAGTAACCCTAGAAGACTTATATAAT
GGTGCAACAAGAAAACTGGCTCTGCAAAAGAATGTGATTTGTGACAAATGTGAAGGTAGA
GGAGGTAAGAAAGGAGCAGTAGAGTGCTGTCCCAATTGCCGAGGTACTGGAATGCAAATA
AGAATTCATCAGATAGGACCTGGAATGGTTCAGCAAATTCAGTCTGTGTGCATGGAGTGC
CAGGGCCATGGGGAGCGGATCAGTCCTAAAGATAGATGTAAAAGCTGCAACGGAAGGAAG
ATAGTTCGAGAGAAGAAAATTTTAGAAGTTCATATTGACAAAGGCATGAAAGATGGCCAG
AAGATAACATTCCATGGTGAAGGAGACCAAGAACCAGGACTGGAGCCAGGCGATATTATC
ATTGTGTTAGATCAGAAGGACCATGCTGTTTTTACTCGACGAGGAGAAGACCTTTTCATG
TGTATGGACATACAGCTCGTTGAAGCACTGTGTGGCTTCCAGAAGCCAATATCTACTCTT
GACAACCGAACCATCGTCATCACCTCTCATCCAGGTCAGATTGTCAAGCATGGAGATATC
AAGTGTGTACTAAATGAAGGCATGCCAATTTATCGTAGACCATATGAAAAGGGTCGCCTA
ATCATCGAATTTAAGGTAAACTTTCCTGAGAATGGCTTTCTCTCTCCTGATAAACTGTCT
TTGCTGGAAAAACTCCTACCCGAGAGGAAGGAAGTGGAAGAGACTGATGAGATGGACCAA
GTAGAACTGGTGGACTTTGATCCAAATCAGGAAAGACGGCGCCACTACAATGGAGAAGCA
TATGAGGATGATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1298
Start on reference sequence 105
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DNAJA1
Symbol Nomenclature : DNAJA1
SYNONYM : DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
Designation : HSJ-2|dnaJ homolog subfamily A member 1|dnaJ protein homolog 2|heat shock 40 kDa protein 4|heat shock protein J2|heat shock protein, DNAJ-like 2|human DnaJ protein 2|neural precursor cell expressed, developmentally down-regulated 7
Full Nomenclature : DnaJ (Hsp40) homolog, subfamily A, member 1
GENEID : 3301
Locus Tag : RP11-54K16.1
HGNC : 5229
MIM : 602837
Vega : OTTHUMG00000019760
Target GenBank: BC008182

 

Reference Sequence Alignment

  

HsCD00075966      ATGGTGAAAGAAACAACTTACTACGATGTTTTGGGGGTCAAACCCAATGCTACTCAGGAA
NM_001539.2       ATGGTGAAAGAAACAACTTACTACGATGTTTTGGGGGTCAAACCCAATGCTACTCAGGAA
                  ************************************************************

HsCD00075966      GAATTGAAAAAGGCTTATAGGAAACTGGCCTTGAAGTACCATCCTGATAAGAACCCAAAT
NM_001539.2       GAATTGAAAAAGGCTTATAGGAAACTGGCTTTGAAGTACCATCCTGATAAGAACCCAAAT
                  ***************************** ******************************

HsCD00075966      GAAGGAGAGAAGTTTAAACAGATTTCTCAAGCTTACGAAGTTCTCTCTGATGCAAAGAAA
NM_001539.2       GAAGGAGAGAAGTTTAAACAGATTTCTCAAGCTTACGAAGTTCTCTCTGATGCAAAGAAA
                  ************************************************************

HsCD00075966      AGGGAATTATATGACAAAGGAGGAGAACAGGCAATTAAAGAGGGTGGAGCAGGTGGCGGT
NM_001539.2       AGGGAATTATATGACAAAGGAGGAGAACAGGCAATTAAAGAGGGTGGAGCAGGTGGCGGT
                  ************************************************************

HsCD00075966      TTTGGCTCCCCCATGGACATCTTTGATATGTTTTTTGGAGGAGGAGGAAGGATGCAGAGA
NM_001539.2       TTTGGCTCCCCCATGGACATCTTTGATATGTTTTTTGGAGGAGGAGGAAGGATGCAGAGA
                  ************************************************************

HsCD00075966      GAAAGGAGAGGTAAAAATGTTGTACATCAGCTCTCAGTAACCCTAGAAGACTTATATAAT
NM_001539.2       GAAAGGAGAGGTAAAAATGTTGTACATCAGCTCTCAGTAACCCTAGAAGACTTATATAAT
                  ************************************************************

HsCD00075966      GGTGCAACAAGAAAACTGGCTCTGCAAAAGAATGTGATTTGTGACAAATGTGAAGGTAGA
NM_001539.2       GGTGCAACAAGAAAACTGGCTCTGCAAAAGAATGTGATTTGTGACAAATGTGAAGGTAGA
                  ************************************************************

HsCD00075966      GGAGGTAAGAAAGGAGCAGTAGAGTGCTGTCCCAATTGCCGAGGTACTGGAATGCAAATA
NM_001539.2       GGAGGTAAGAAAGGAGCAGTAGAGTGCTGTCCCAATTGCCGAGGTACTGGAATGCAAATA
                  ************************************************************

HsCD00075966      AGAATTCATCAGATAGGACCTGGAATGGTTCAGCAAATTCAGTCTGTGTGCATGGAGTGC
NM_001539.2       AGAATTCATCAGATAGGACCTGGAATGGTTCAGCAAATTCAGTCTGTGTGCATGGAGTGC
                  ************************************************************

HsCD00075966      CAGGGCCATGGGGAGCGGATCAGTCCTAAAGATAGATGTAAAAGCTGCAACGGAAGGAAG
NM_001539.2       CAGGGCCATGGGGAGCGGATCAGTCCTAAAGATAGATGTAAAAGCTGCAACGGAAGGAAG
                  ************************************************************

HsCD00075966      ATAGTTCGAGAGAAGAAAATTTTAGAAGTTCATATTGACAAAGGCATGAAAGATGGCCAG
NM_001539.2       ATAGTTCGAGAGAAGAAAATTTTAGAAGTTCATATTGACAAAGGCATGAAAGATGGCCAG
                  ************************************************************

HsCD00075966      AAGATAACATTCCATGGTGAAGGAGACCAAGAACCAGGACTGGAGCCAGGCGATATTATC
NM_001539.2       AAGATAACATTCCATGGTGAAGGAGACCAAGAACCAGGACTGGAGCCAGGCGATATTATC
                  ************************************************************

HsCD00075966      ATTGTGTTAGATCAGAAGGACCATGCTGTTTTTACTCGACGAGGAGAAGACCTTTTCATG
NM_001539.2       ATTGTGTTAGATCAGAAGGACCATGCTGTTTTTACTCGACGAGGAGAAGACCTTTTCATG
                  ************************************************************

HsCD00075966      TGTATGGACATACAGCTCGTTGAAGCACTGTGTGGCTTCCAGAAGCCAATATCTACTCTT
NM_001539.2       TGTATGGACATACAGCTCGTTGAAGCACTGTGTGGCTTCCAGAAGCCAATATCTACTCTT
                  ************************************************************

HsCD00075966      GACAACCGAACCATCGTCATCACCTCTCATCCAGGTCAGATTGTCAAGCATGGAGATATC
NM_001539.2       GACAACCGAACCATCGTCATCACCTCTCATCCAGGTCAGATTGTCAAGCATGGAGATATC
                  ************************************************************

HsCD00075966      AAGTGTGTACTAAATGAAGGCATGCCAATTTATCGTAGACCATATGAAAAGGGTCGCCTA
NM_001539.2       AAGTGTGTACTAAATGAAGGCATGCCAATTTATCGTAGACCATATGAAAAGGGTCGCCTA
                  ************************************************************

HsCD00075966      ATCATCGAATTTAAGGTAAACTTTCCTGAGAATGGCTTTCTCTCTCCTGATAAACTGTCT
NM_001539.2       ATCATCGAATTTAAGGTAAACTTTCCTGAGAATGGCTTTCTCTCTCCTGATAAACTGTCT
                  ************************************************************

HsCD00075966      TTGCTGGAAAAACTCCTACCCGAGAGGAAGGAAGTGGAAGAGACTGATGAGATGGACCAA
NM_001539.2       TTGCTGGAAAAACTCCTACCCGAGAGGAAGGAAGTGGAAGAGACTGATGAGATGGACCAA
                  ************************************************************

HsCD00075966      GTAGAACTGGTGGACTTTGATCCAAATCAGGAAAGACGGCGCCACTACAATGGAGAAGCA
NM_001539.2       GTAGAACTGGTGGACTTTGATCCAAATCAGGAAAGACGGCGCCACTACAATGGAGAAGCA
                  ************************************************************

HsCD00075966      TATGAGGATGATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTTG
NM_001539.2       TATGAGGATGATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAA
                  ****************************************************:.

Pathways

NCI : Regulation of Androgen receptor activity
WikiPathway : Diurnally regulated genes with circadian orthologs

Protein

SMART domain : DnaJ : DnaJ molecular chaperone homology domain
UniProt : Q5T7Q0
UniProt : P31689
HPRD : 04159

Clone

Cloning Information : 169265
HIP Master Clone ID : 142731
Original Clone ID : FLH169265.01L

Organism


TAX_ID : 9606
Species Specific ID: 3301

Genome

Chromosome : 9
Map Location : 9p13.3
Ensembl : ENSG00000086061

Reagents

Labome : DNAJA1-antibody