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WDR31 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  WDR31
Gene Name:  WD repeat domain 31
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH263887.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00076410

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1101nts         Open reading frame : 1 to 1101
Reference Sequence Alignment

ATGCTGCTACTCAGGTGCCAACTGAAACAAGCTCCTCCACAGAAGGTTTCGTTTAGGTTT
TGTGTCGTGATGGGGAAACAGCAAAGCAAACTCAAACACAGCACTTATAAATACGGGCCT
GATGAAATTATAGAAGAGAGAATTCAAACTAAAGCTTTTCAAGAGTATAGCCCAGCTCAC
ATGGATACCGTCTCTGTCGTGGCTGCTTTGAACTCAGACCTTTGTGTCTCTGGAGGGAAA
GATAAGACAGTTGTGGCCTATAATTGGAAAACTGGAAATGTGGTGAAAAGGTTCAAAGGA
CATGAACATGAGATCACCAAGGTAGCCTGTATTCCCAAATCCAGCCAGTTCTTCAGTGCC
TCTCGTGACAGGATGGTCATGATGTGGGACTTGCACGGTTCCTCACAACCAAGGCAGCAA
TTGTGTGGCCATGCCATGGTGGTCACCGGATTGGCTGTGAGTCCAGACTCATCACAGCTG
TGCACTGGCTCTCGGGACAACACCCTGCTTCTGTGGGATGTGGTGACAGGACAGAGTGTG
GAAAGAGCATCTGTCTCCAGGAACGTGGTCACTCACCTGTGCTGGGTCCCCAGAGAACCA
TACATACTACAGACCTCTGAAGATAAAACCCTCAGATTATGGGACAGTCGGGGGCTGCAG
GTAGCTCATATGTTTCCTGCAAAGCAGCACATTCAGACCTACTGTGAAGTCAGTGTGGAT
GGACACAAGTGTATCTCCTGCAGCAATGGCTTTGGAGGAGAAGGCTGTGAAGCCACGTTG
TGGGACCTAAGACAGACTCGAAACAGAATATGTGAGTATAAGGGGCATTTCCAGACTGTC
GCATCCTGCGTCTTTCTACCAAGAGCATTGGCCTTGATGCCTTTAATTGCTACCTCATCA
CATGATTGCAAGGTGAAGATTTGGAACCAAGATACTGGAGCCTGCCTTTTCACCTTGTCT
CTGGATGGATCAGGACCCTTGACTTCTCTGGCTGTTGGTGACGCCATCTCCTTATTGTGT
GCAAGTTTTAACAGAGGAATTCACTTACTCAGAATGGACCACAGCCAAGGGCTGGAACTG
CAGGAAGTGGCAGCATTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1338
Start on reference sequence 238
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : WDR31
Symbol Nomenclature : WDR31
Designation : WD repeat-containing protein 31
Full Nomenclature : WD repeat domain 31
GENEID : 114987
HGNC : 21421
Vega : OTTHUMG00000020525
Target GenBank: BC012352

 

Reference Sequence Alignment

  

HsCD00076410        ATGCTGCTACTCAGGTGCCAACTGAAACAAGCTCCTCCACAGAAGGTTTCGTTTAGGTTT
XM_005251690.1      ATGCTGCTACTCAGGTGCCAACTGAAACAAGCTCCTCCACAGAAGGTTTCGTTTAGGTTT
NM_145241.3         ATGCTGCTACTCAGGTGCCAACTGAAACAAGCTCCTCCACAGAAGGTTTCGTTTAGGTTT
NM_001012361.2      ATGCTGCTACTCAGGTGCCAACTGAAACAAGCTCCTCCACAGAAGGTTTCGTTTAGGTTT
                    ************************************************************

HsCD00076410        TGTGTCGTGATGGGGAAACAGCAAAGCAAACTCAAACACAGCACTTATAAATACG---GG
XM_005251690.1      TGTGTCGTGATGGGGAAACAGCAAAGCAAACTCAAACACAGCACTTATAAATACGGCAGG
NM_145241.3         TGTGTCGTGATGGGGAAACAGCAAAGCAAACTCAAACACAGCACTTATAAATACG---GG
NM_001012361.2      TGTGTCGTGATGGGGAAACAGCAAAGCAAACTCAAACACAGCACTTATAAATACGGCAGG
                    *******************************************************   **

HsCD00076410        CCTGATGAAATTATAGAAGAGAGAATTCAAACTAAAGCTTTTCAAGAGTATAGCCCAGCT
XM_005251690.1      CCTGATGAAATTATAGAAGAGAGAATTCAAACTAAAGCTTTTCAAGAGTATAGCCCAGCT
NM_145241.3         CCTGATGAAATTATAGAAGAGAGAATTCAAACTAAAGCTTTTCAAGAGTATAGCCCAGCT
NM_001012361.2      CCTGATGAAATTATAGAAGAGAGAATTCAAACTAAAGCTTTTCAAGAGTATAGCCCAGCT
                    ************************************************************

HsCD00076410        CACATGGATACCGTCTCTGTCGTGGCTGCTTTGAACTCAGACCTTTGTGTCTCTGGAGGG
XM_005251690.1      CACATGGATACCGTCTCTGTCGTGGCTGCTTTGAACTCAGACCTTTGTGTCTCTGGAGGG
NM_145241.3         CACATGGATACCGTCTCTGTCGTGGCTGCTTTGAACTCAGACCTTTGTGTCTCTGGAGGG
NM_001012361.2      CACATGGATACCGTCTCTGTCGTGGCTGCTTTGAACTCAGACCTTTGTGTCTCTGGAGGG
                    ************************************************************

HsCD00076410        AAAGATAAGACAGTTGTGGCCTATAATTGGAAAACTGGAAATGTGGTGAAAAGGTTCAAA
XM_005251690.1      AAAGATAAGACAGTTGTGGCCTATAATTGGAAAACTGGAAATGTGGTGAAAAGGTTCAAA
NM_145241.3         AAAGATAAGACAGTTGTGGCCTATAATTGGAAAACTGGAAATGTGGTGAAAAGGTTCAAA
NM_001012361.2      AAAGATAAGACAGTTGTGGCCTATAATTGGAAAACTGGAAATGTGGTGAAAAGGTTCAAA
                    ************************************************************

HsCD00076410        GGACATGAACATGAGATCACCAAGGTAGCCTGTATTCCCAAATCCAGCCAGTTCTTCAGT
XM_005251690.1      GGACATGAACATGAGATCACCAAGGTAGCCTGTATTCCCAAATCCAGCCAGTTCTTCAGT
NM_145241.3         GGACATGAACATGAGATCACCAAGGTAGCCTGTATTCCCAAATCCAGCCAGTTCTTCAGT
NM_001012361.2      GGACATGAACATGAGATCACCAAGGTAGCCTGTATTCCCAAATCCAGCCAGTTCTTCAGT
                    ************************************************************

HsCD00076410        GCCTCTCGTGACAGGATGGTCATGATGTGGGACTTGCACGGTTCCTCACAACCAAGGCAG
XM_005251690.1      GCCTCTCGTGACAGGATGGTCATGATGTGGGACTTGCACGGTTCCTCACAACCAAGGCAG
NM_145241.3         GCCTCTCGTGACAGGATGGTCATGATGTGGGACTTGCACGGTTCCTCACAACCAAGGCAG
NM_001012361.2      GCCTCTCGTGACAGGATGGTCATGATGTGGGACTTGCACGGTTCCTCACAACCAAGGCAG
                    ************************************************************

HsCD00076410        CAATTGTGTGGCCATGCCATGGTGGTCACCGGATTGGCTGTGAGTCCAGACTCATCACAG
XM_005251690.1      CAATTGTGTGGCCATGCCATGGTGGTCACCGGATTGGCTGTGAGTCCAGACTCATCACAG
NM_145241.3         CAATTGTGTGGCCATGCCATGGTGGTCACCGGATTGGCTGTGAGTCCAGACTCATCACAG
NM_001012361.2      CAATTGTGTGGCCATGCCATGGTGGTCACCGGATTGGCTGTGAGTCCAGACTCATCACAG
                    ************************************************************

HsCD00076410        CTGTGCACTGGCTCTCGGGACAACACCCTGCTTCTGTGGGATGTGGTGACAGGACAGAGT
XM_005251690.1      CTGTGCACTGGCTCTCGGGACAACACCCTGCTTCTGTGGGATGTGGTGACAGGACAGAGT
NM_145241.3         CTGTGCACTGGCTCTCGGGACAACACCCTGCTTCTGTGGGATGTGGTGACAGGACAGAGT
NM_001012361.2      CTGTGCACTGGCTCTCGGGACAACACCCTGCTTCTGTGGGATGTGGTGACAGGACAGAGT
                    ************************************************************

HsCD00076410        GTGGAAAGAGCATCTGTCTCCAGGAACGTGGTCACTCACCTGTGCTGGGTCCCCAGAGAA
XM_005251690.1      GTGGAAAGAGCATCTGTCTCCAGGAACGTGGTCACTCACCTGTGCTGGGTCCCCAGAGAA
NM_145241.3         GTGGAAAGAGCATCTGTCTCCAGGAACGTGGTCACTCACCTGTGCTGGGTCCCCAGAGAA
NM_001012361.2      GTGGAAAGAGCATCTGTCTCCAGGAACGTGGTCACTCACCTGTGCTGGGTCCCCAGAGAA
                    ************************************************************

HsCD00076410        CCATACATACTACAGACCTCTGAAGATAAAACCCTCAGATTATGGGACAGTCGGGGGCTG
XM_005251690.1      CCATACATACTACAGACCTCTGAAGATAAAACCCTCAGATTATGGGACAGTCGGGGGCTG
NM_145241.3         CCATACATACTACAGACCTCTGAAGATAAAACCCTCAGATTATGGGACAGTCGGGGGCTG
NM_001012361.2      CCATACATACTACAGACCTCTGAAGATAAAACCCTCAGATTATGGGACAGTCGGGGGCTG
                    ************************************************************

HsCD00076410        CAGGTAGCTCATATGTTTCCTGCAAAGCAGCACATTCAGACCTACTGTGAAGTCAGTGTG
XM_005251690.1      CAGGTAGCTCATATGTTTCCTGCAAAGCAGCACATTCAGACCTACTGTGAAGTCAGTGTG
NM_145241.3         CAGGTAGCTCATATGTTTCCTGCAAAGCAGCACATTCAGACCTACTGTGAAGTCAGTGTG
NM_001012361.2      CAGGTAGCTCATATGTTTCCTGCAAAGCAGCACATTCAGACCTACTGTGAAGTCAGTGTG
                    ************************************************************

HsCD00076410        GATGGACACAAGTGTATCTCCTGCAGCAATGGCTTTGGAGGAGAAGGCTGTGAAGCCACG
XM_005251690.1      GATGGACACAAGTGTATCTCCTGCAGCAATGGCTTTGGAGGAGAAGGCTGTGAAGCCACG
NM_145241.3         GATGGACACAAGTGTATCTCCTGCAGCAATGGCTTTGGAGGAGAAGGCTGTGAAGCCACG
NM_001012361.2      GATGGACACAAGTGTATCTCCTGCAGCAATGGCTTTGGAGGAGAAGGCTGTGAAGCCACG
                    ************************************************************

HsCD00076410        TTGTGGGACCTAAGACAGACTCGAAACAGAATATGTGAGTATAAGGGGCATTTCCAGACT
XM_005251690.1      TTGTGGGACCTAAGACAGACTCGAAACAGAATATGTGAGTATAAGGGGCATTTCCAGACT
NM_145241.3         TTGTGGGACCTAAGACAGACTCGAAACAGAATATGTGAGTATAAGGGGCATTTCCAGACT
NM_001012361.2      TTGTGGGACCTAAGACAGACTCGAAACAGAATATGTGAGTATAAGGGGCATTTCCAGACT
                    ************************************************************

HsCD00076410        GTCGCATCCTGCGTCTTTCTACCAAGAGCATTGGCCTTGATGCCTTTAATTGCTACCTCA
XM_005251690.1      GTCGCATCCTGCGTCTTTCTACCAAGAGCATTGGCCTTGATGCCTTTAATTGCTACCTCA
NM_145241.3         GTCGCATCCTGCGTCTTTCTACCAAGAGCATTGGCCTTGATGCCTTTAATTGCTACCTCA
NM_001012361.2      GTCGCATCCTGCGTCTTTCTACCAAGAGCATTGGCCTTGATGCCTTTAATTGCTACCTCA
                    ************************************************************

HsCD00076410        TCACATGATTGCAAGGTGAAGATTTGGAACCAAGATACTGGAGCCTGCCTTTTCACCTTG
XM_005251690.1      TCACATGATTGCAAGGTGAAGATTTGGAACCAAGATACTGGAGCCTGCCTTTTCACCTTG
NM_145241.3         TCACATGATTGCAAGGTGAAGATTTGGAACCAAGATACTGGAGCCTGCCTTTTCACCTTG
NM_001012361.2      TCACATGATTGCAAGGTGAAGATTTGGAACCAAGATACTGGAGCCTGCCTTTTCACCTTG
                    ************************************************************

HsCD00076410        TCTCTGGATGGATCAGGACCCTTGACTTCTCTGGCTGTTGGTGACGCCATCTCCTTATTG
XM_005251690.1      TCTCTGGATGGATCAGGACCCTTGACTTCTCTGGCTGTTGGTGACGCCATCTCCTTATTG
NM_145241.3         TCTCTGGATGGATCAGGACCCTTGACTTCTCTGGCTGTTGGTGACGCCATCTCCTTATTG
NM_001012361.2      TCTCTGGATGGATCAGGACCCTTGACTTCTCTGGCTGTTGGTGACGCCATCTCCTTATTG
                    ************************************************************

HsCD00076410        TGTGCAAGTTTTAACAGAGGAATTCACTTACTCAGAATGGACCACAGCCAAGGGCTGGAA
XM_005251690.1      TGTGCAAGTTTTAACAGAGGAATTCACTTACTCAGAATGGACCACAGCCAAGGGCTGGAA
NM_145241.3         TGTGCAAGTTTTAACAGAGGAATTCACTTACTCAGAATGGACCACAGCCAAGGGCTGGAA
NM_001012361.2      TGTGCAAGTTTTAACAGAGGAATTCACTTACTCAGAATGGACCACAGCCAAGGGCTGGAA
                    ************************************************************

HsCD00076410        CTGCAGGAAGTGGCAGCATTCTTG
XM_005251690.1      CTGCAGGAAGTGGCAGCATTCTGA
NM_145241.3         CTGCAGGAAGTGGCAGCATTCTGA
NM_001012361.2      CTGCAGGAAGTGGCAGCATTCTGA
                    ********************** .

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q8NA23
HPRD : 18299

Clone

Cloning Information : 263887
HIP Master Clone ID : 196581
Original Clone ID : FLH263887.01L

Organism


TAX_ID : 9606
Species Specific ID: 114987

Genome

Chromosome : 9
Map Location : 9q32
Ensembl : ENSG00000148225

Reagents

Labome : WDR31-antibody