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UNRIP (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  STRAP
Gene Name:  serine/threonine kinase receptor associated protein
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH222027.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00076607

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1053nts         Open reading frame : 1 to 1053
Reference Sequence Alignment

ATGGCAATGAGACAGACGCCGCTCACCTGCTCTGGCCACACGCGACCCGTGGTTGATTTG
GCCTTCAGTGGCATCACGCCTTATGGGTATTTCTTAATCAGCGCTTGCAAAGATGGTAAA
CCTATGCTACGCCAGGGAGATACAGGAGACTGGATTGGAACATTTTTGGGTCATAAAGGT
GCTGTTTGGGGTGCAACACTGAATAAGGATGCCACCAAAGCAGCTACAGCAGCTGCAGAT
TTCACAGCCAAAGTGTGGGATGCTGTCTCAGGAGATGAATTGATGACCCTGGCTCATAAA
CACATTGTCAAGACTGTGGATTTCACGCAGGATAGTAATTATTTGTTAACCGGGGGACAG
GATAAACTGTTACGCATATATGACTTGAACAAACCTGAAGCAGAACCTAAGGAAATTAGT
GGTCATACTTCTGGTATAAAAAAAGCTCTGTGGTGCAGTGAGGATAAACAGATTCTTTCT
GCTGATGACAAAACTGTTCGACTTTGGGATCATGCTACTATGACAGAAGTGAAATCTCTA
AATTTTAATATGTCTGTTAGTAGTATGGAATATATTCCTGAGGGAGAGATTTTGGTTATA
ACTTATGGACGATCTATTGCTTTTCATAGTGCAGTAAGTTTGGACCCAATTAAATCCTTT
GAAGCTCCTGCAACCATCAATTCTGCATCTCTTCATCCTGAGAAAGAATTTCTTGTTGCA
GGCGGTGAAGATTTTAAACTTTATAAGTATGATTATAATAGTGGAGAAGAATTAGAATCC
TACAAGGGACACTTTGGTCCTATTCACTGTGTGAGATTTAGTCCTGATGGAGAACTCTAT
GCCAGTGGTTCAGAAGATGGAACATTGAGACTATGGCAAACTGTGGTAGGAAAAACGTAT
GGCCTTTGGAAATGTGTGCTTCCTGAAGAAGATAGTGGTGAGCTGGCAAAGCCAAAGATT
GGTTTTCCAGAGACAACAGAAGAGGAGCTAGAAGAAATTGCTTCAGAGAATTCAGATTGC
ATCTTTCCTTCAGCTCCTGATGTTAAGGCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1198
Start on reference sequence 146
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STRAP
Symbol Nomenclature : STRAP
SYNONYM : MAWD; PT-WD; UNRIP
Designation : MAP activator with WD repeats|WD-40 repeat protein PT-WD|serine-threonine kinase receptor-associated protein|unr-interacting protein
Full Nomenclature : serine/threonine kinase receptor associated protein
GENEID : 11171
HGNC : 30796
MIM : 605986
Vega : OTTHUMG00000168791
Target GenBank: BC000162

 

Reference Sequence Alignment

  

HsCD00076607      ATGGCAATGAGACAGACGCCGCTCACCTGCTCTGGCCACACGCGACCCGTGGTTGATTTG
NM_007178.3       ATGGCAATGAGACAGACGCCGCTCACCTGCTCTGGCCACACGCGACCCGTGGTTGATTTG
                  ************************************************************

HsCD00076607      GCCTTCAGTGGCATCACGCCTTATGGGTATTTCTTAATCAGCGCTTGCAAAGATGGTAAA
NM_007178.3       GCCTTCAGTGGCATCACGCCTTATGGGTATTTCTTAATCAGCGCTTGCAAAGATGGTAAA
                  ************************************************************

HsCD00076607      CCTATGCTACGCCAGGGAGATACAGGAGACTGGATTGGAACATTTTTGGGTCATAAAGGT
NM_007178.3       CCTATGCTACGCCAGGGAGATACAGGAGACTGGATTGGAACATTTTTGGGTCATAAAGGT
                  ************************************************************

HsCD00076607      GCTGTTTGGGGTGCAACACTGAATAAGGATGCCACCAAAGCAGCTACAGCAGCTGCAGAT
NM_007178.3       GCTGTTTGGGGTGCAACACTGAATAAGGATGCCACCAAAGCAGCTACAGCAGCTGCAGAT
                  ************************************************************

HsCD00076607      TTCACAGCCAAAGTGTGGGATGCTGTCTCAGGAGATGAATTGATGACCCTGGCTCATAAA
NM_007178.3       TTCACAGCCAAAGTGTGGGATGCTGTCTCAGGAGATGAATTGATGACCCTGGCTCATAAA
                  ************************************************************

HsCD00076607      CACATTGTCAAGACTGTGGATTTCACGCAGGATAGTAATTATTTGTTAACCGGGGGACAG
NM_007178.3       CACATTGTCAAGACTGTGGATTTCACGCAGGATAGTAATTATTTGTTAACCGGGGGACAG
                  ************************************************************

HsCD00076607      GATAAACTGTTACGCATATATGACTTGAACAAACCTGAAGCAGAACCTAAGGAAATTAGT
NM_007178.3       GATAAACTGTTACGCATATATGACTTGAACAAACCTGAAGCAGAACCTAAGGAAATTAGT
                  ************************************************************

HsCD00076607      GGTCATACTTCTGGTATAAAAAAAGCTCTGTGGTGCAGTGAGGATAAACAGATTCTTTCT
NM_007178.3       GGTCATACTTCTGGTATAAAAAAAGCTCTGTGGTGCAGTGAGGATAAACAGATTCTTTCT
                  ************************************************************

HsCD00076607      GCTGATGACAAAACTGTTCGACTTTGGGATCATGCTACTATGACAGAAGTGAAATCTCTA
NM_007178.3       GCTGATGACAAAACTGTTCGACTTTGGGATCATGCTACTATGACAGAAGTGAAATCTCTA
                  ************************************************************

HsCD00076607      AATTTTAATATGTCTGTTAGTAGTATGGAATATATTCCTGAGGGAGAGATTTTGGTTATA
NM_007178.3       AATTTTAATATGTCTGTTAGTAGTATGGAATATATTCCTGAGGGAGAGATTTTGGTTATA
                  ************************************************************

HsCD00076607      ACTTATGGACGATCTATTGCTTTTCATAGTGCAGTAAGTTTGGACCCAATTAAATCCTTT
NM_007178.3       ACTTATGGACGATCTATTGCTTTTCATAGTGCAGTAAGTTTGGACCCAATTAAATCCTTT
                  ************************************************************

HsCD00076607      GAAGCTCCTGCAACCATCAATTCTGCATCTCTTCATCCTGAGAAAGAATTTCTTGTTGCA
NM_007178.3       GAAGCTCCTGCAACCATCAATTCTGCATCTCTTCATCCTGAGAAAGAATTTCTTGTTGCA
                  ************************************************************

HsCD00076607      GGCGGTGAAGATTTTAAACTTTATAAGTATGATTATAATAGTGGAGAAGAATTAGAATCC
NM_007178.3       GGCGGTGAAGATTTTAAACTTTATAAGTATGATTATAATAGTGGAGAAGAATTAGAATCC
                  ************************************************************

HsCD00076607      TACAAGGGACACTTTGGTCCTATTCACTGTGTGAGATTTAGTCCTGATGGAGAACTCTAT
NM_007178.3       TACAAGGGACACTTTGGTCCTATTCACTGTGTGAGATTTAGTCCTGATGGAGAACTCTAT
                  ************************************************************

HsCD00076607      GCCAGTGGTTCAGAAGATGGAACATTGAGACTATGGCAAACTGTGGTAGGAAAAACGTAT
NM_007178.3       GCCAGTGGTTCAGAAGATGGAACATTGAGACTATGGCAAACTGTGGTAGGAAAAACGTAT
                  ************************************************************

HsCD00076607      GGCCTTTGGAAATGTGTGCTTCCTGAAGAAGATAGTGGTGAGCTGGCAAAGCCAAAGATT
NM_007178.3       GGCCTTTGGAAATGTGTGCTTCCTGAAGAAGATAGTGGTGAGCTGGCAAAGCCAAAGATT
                  ************************************************************

HsCD00076607      GGTTTTCCAGAGACAACAGAAGAGGAGCTAGAAGAAATTGCTTCAGAGAATTCAGATTGC
NM_007178.3       GGTTTTCCAGAGACAACAGAAGAGGAGCTAGAAGAAATTGCTTCAGAGAATTCAGATTGC
                  ************************************************************

HsCD00076607      ATCTTTCCTTCAGCTCCTGATGTTAAGGCCTTG
NM_007178.3       ATCTTTCCTTCAGCTCCTGATGTTAAGGCCTGA
                  ******************************* .

Pathways

NCI : TGF-beta receptor signaling
Reactome : Disease
Reactome : Downregulation of TGF-beta receptor signaling
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGF-beta receptor signaling activates SMADs
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q9Y3F4
HPRD : 05816

Clone

Cloning Information : 222027
HIP Master Clone ID : 141359
Original Clone ID : FLH222027.01L

Organism


TAX_ID : 9606
Species Specific ID: 11171

Genome

Chromosome : 12
Map Location : 12p12.3
Ensembl : ENSG00000023734

Reagents

Labome : STRAP-antibody