Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00779364
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3609nts
Open reading frame: 1 to 3609
ATGACTCGATCGCCGCCCCTCAGAGAGCTGCCCCCGAGTTACACACCCCCAGCTCGAACC
GCAGCACCCCAGATCCTAGCTGGGAGCCTGAAGGCTCCACTCTGGCTTCGTGCTTACTTC
CAGGGCCTGCTCTTCTCTCTGGGATGCGGGATCCAGAGACATTGTGGCAAAGTGCTCTTT
CTGGGACTGTTGGCCTTTGGGGCCCTGGCATTAGGTCTCCGCATGGCCATTATTGAGACA
AACTTGGAACAGCTCTGGGTAGAAGTGGGCAGCCGGGTGAGCCAGGAGCTGCATTACACC
AAGGAGAAGCTGGGGGAGGAGGCTGCATACACCTCTCAGATGCTGATACAGACCGCACGC
CAGGAGGGAGAGAACATCCTCACACCCGAAGCACTTGGCCTCCACCTCCAGGCAGCCCTC
ACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAGTCCTGGGATTTGAACAAAATCTGC
TACAAGTCAGGAGTTCCCCTTATTGAAAATGGAATGATTGAGCGGATGATTGAGAAGCTG
TTTCCGTGCGTGATCCTCACCCCCCTCGACTGCTTCTGGGAGGGAGCCAAACTCCAAGGG
GGCTCCGCCTACCTGCCCGGCCGCCCGGATATCCAGTGGACCAACCTGGATCCAGAGCAG
CTGCTGGAGGAGCTGGGTCCCTTTGCCTCCCTTGAGGGCTTCCGGGAGCTGCTAGACAAG
GCACAGGTGGGCCAGGCCTACGTGGGGCGGCCCTGTCTGCACCCTGATGACCTCCACTGC
CCACCTAGTGCCCCCAACCATCACAGCAGGCAGGCTCCCAATGTGGCTCACGAGCTGAGT
GGGGGCTGCCATGGCTTCTCCCACAAATTCATGCACTGGCAGGAGGAATTGCTGCTGGGA
GGCATGGCCAGAGACCCCCAAGGAGAGCTGCTGAGGGCAGAGGCCCTGCAGAGCACCTTC
TTGCTGATGAGTCCCCGCCAGCTGTACGAGCATTTCCGGGGTGACTATCAGACACATGAC
ATTGGCTGGAGTGAGGAGCAGGCCAGCACAGTGCTACAAGCCTGGCAGCGGCGCTTTGTG
CAGCTGGCCCAGGAGGCCCTGCCTGAGAACGCTTCCCAGCAGATCCATGCCTTCTCCTCC
ACCACCCTGGATGACATCCTGCATGCGTTCTCTGAAGTCAGTGCTGCCCGTGTGGTGGGA
GGCTATCTGCTCATGCTGGCCTATGCCTGTGTGACCATGCTGCGGTGGGACTGCGCCCAG
TCCCAGGGTTCCGTGGGCCTTGCCGGGGTACTGCTGGTGGCCCTGGCGGTGGCCTCAGGC
CTTGGGCTCTGTGCCCTGCTCGGCATCACCTTCAATGCTGCCACTACCCAGGTGCTGCCC
TTCTTGGCTCTGGGAATCGGCGTGGATGACGTATTCCTGCTGGCGCATGCCTTCACAGAG
GCTCTGCCTGGCACCCCTCTCCAGGAGCGCATGGGCGAGTGTCTGCAGCGCACGGGCACC
AGTGTCGTACTCACATCCATCAACAACATGGCCGCCTTCCTCATGGCTGCCCTCGTTCCC
ATCCCTGCGCTGCGAGCCTTCTCCCTACAGGCGGCCATAGTGGTTGGCTGCACCTTTGTA
GCCGTGATGCTTGTCTTCCCAGCCATCCTCAGCCTGGACCTACGGCGGCGCCACTGCCAG
CGCCTTGATGTGCTCTGCTGCTTCTCCAGTCCCTGCTCTGCTCAGGTGATTCAGATCCTG
CCCCAGGAGCTGGGGGACGGGACAGTACCAGTGGGCATTGCCCACCTCACTGCCACAGTT
CAAGCCTTTACCCACTGTGAAGCCAGCAGCCAGCATGTGGTCACCATCCTGCCTCCCCAA
GCCCACCTGGTGCCCCCACCTTCTGACCCACTGGGCTCTGAGCTCTTCAGCCCTGGAGGG
TCCACACGGGACCTTCTAGGCCAGGAGGAGGAGACAAGGCAGAAGGCAGCCTGCAAGTCC
CTGCCCTGTGCCCGCTGGAATCTTGCCCATTTCGCCCGCTATCAGTTTGCCCCGTTGCTG
CTCCAGTCACATGCTAAGGCCATCGTGCTGGTGCTCTTTGGTGCTCTTCTGGGCCTGAGC
CTCTACGGAGCCACCTTGGTGCAAGACGGCCTGGCCCTGACGGATGTGGTGCCTCGGGGC
ACCAAGGAGCATGCCTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTGTACGAGGTGGCC
CTGGTGACCCAGGGTGGCTTTGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTGCAC
CAGCGCTTCAGTTCCCTCAAGGCGGTGCTGCCCCCACCGGCCACCCAGGCACCCCGCACC
TGGCTGCACTATTACCGCAACTGGCTACAGGGAATCCAGGCTGCCTTTGACCAGGACTGG
GCTTCTGGGCGCATCACCCGCCACTCGTACCGCAATGGCTCTGAGGATGGGGCCCTGGCC
TACAAGCTGCTCATCCAGACTGGAGACGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACC
ACAAGGAAGCTGGTGGACAGAGAGGGACTGATTCCACCCGAGCTCTTCTACATGGGGCTG
ACCGTGTGGGTGAGCAGTGACCCCCTGGGTCTGGCAGCCTCACAGGCCAACTTCTACCCC
CCACCTCCTGAATGGCTGCACGACAAATACGACACCACGGGGGAGAACCTTCGCATCCCG
CCAGCTCAGCCCTTGGAGTTTGCCCAGTTCCCCTTCCTGCTGCGTGGCCTCCAGAAGACT
GCAGACTTTGTGGAGGCCATCGAGGGGGCCCGGGCAGCATGCGCAGAGGCCGGCCAGGCT
GGGGTGCACGCCTACCCCAGCGGCTCCCCCTTCCTCTTCTGGGAACAGTATCTGGGCCTG
CGGCGCTGCTTCCTGCTGGCCGTCTGCATCCTGCTGGTGTGCACTTTCCTCGTCTGTGCT
CTGCTGCTCCTCAACCCCTGGACGGCTGGCCTCATAGTGCTGGTCCTGGCGATGATGACA
GTGGAACTCTTTGGTATCATGGGTTTCCTGGGCATCAAGCTGAGTGCCATCCCCGTGGTG
ATCCTTGTGGCCTCTGTAGGCATTGGCGTTGAGTTCACAGTCCACGTGGCTCTGGGCTTC
CTGACCACCCAGGGCAGCCGGAACCTGCGGGCCGCCCATGCCCTTGAGCACACATTTGCC
CCCGTGACCGATGGGGCCATCTCCACATTGCTGGGTCTGCTCATGCTTGCTGGTTCCCAC
TTTGACTTCATTGTAAGGTACTTCTTTGCGGCGCTGACAGTGCTCACGCTCCTGGGCCTC
CTCCATGGACTCGTGCTGCTGCCTGTGCTGCTGTCCATCCTGGGCCCGCCGCCAGAGGTG
ATACAGATGTACAAGGAAAGCCCAGAGATCCTGAGTCCACCAGCTCCACAGGGAGGCGGG
CTTAGGTGGGGGGCATCCTCCTCCCTGCCCCAGAGCTTTGCCAGAGTGACTACCTCCATG
ACCGTGGCCATCCACCCACCCCCCCTGCCTGGTGCCTACATCCATCCAGCCCCTGATGAG
CCCCCTTGGTCCCCTGCTGCCACTAGCTCTGGCAACCTCAGTTCCAGGGGACCAGGTCCA
GCCACTGGG
Protein sequence predicted based on nucleotide sequence
MTRSPPLRELPPSYTPPARTAAPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLF LGLLAFGALALGLRMAIIETNLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAR QEGENILTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKL FPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDK AQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAHELSGGCHGFSHKFMHWQEELLLG GMARDPQGELLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASTVLQAWQRRFV QLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQ SQGSVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDVFLLAHAFTE ALPGTPLQERMGECLQRTGTSVVLTSINNMAAFLMAALVPIPALRAFSLQAAIVVGCTFV AVMLVFPAILSLDLRRRHCQRLDVLCCFSSPCSAQVIQILPQELGDGTVPVGIAHLTATV QAFTHCEASSQHVVTILPPQAHLVPPPSDPLGSELFSPGGSTRDLLGQEEETRQKAACKS LPCARWNLAHFARYQFAPLLLQSHAKAIVLVLFGALLGLSLYGATLVQDGLALTDVVPRG TKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRT WLHYYRNWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLIQTGDAQEPLDFSQLT TRKLVDREGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIP PAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEAGQAGVHAYPSGSPFLFWEQYLGL RRCFLLAVCILLVCTFLVCALLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVV ILVASVGIGVEFTVHVALGFLTTQGSRNLRAAHALEHTFAPVTDGAISTLLGLLMLAGSH FDFIVRYFFAALTVLTLLGLLHGLVLLPVLLSILGPPPEVIQMYKESPEILSPPAPQGGG LRWGASSSLPQSFARVTTSMTVAIHPPPLPGAYIHPAPDEPPWSPAATSSGNLSSRGPGP ATG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
