DNASU Plasmid Repository • 480.965.5697 | Email

ACTR1B (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ACTR1B
Gene Name:  ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH226041.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00079036

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1131nts        
Open reading frame : 1 to 1131
Reference Sequence Alignment

ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1207
Start on reference sequence 77
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Al Lau
Niroshan Ramachandran
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR1 AttR1 site for recombination in empty vector 549 565
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR1B
Symbol Nomenclature : ACTR1B
SYNONYM : ARP1B; CTRN2; PC3
Designation : actin-related protein 1B|beta-centractin|centractin beta
Full Nomenclature : ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GENEID : 10120
HGNC : 168
MIM : 605144
Vega : OTTHUMG00000130549
Target GenBank: BC004374

 

Reference Sequence Alignment

  

HsCD00079036      ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005735.3       ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005736.3       ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
                  ************** .* ***************** ** ***********:** ** ***

HsCD00079036      ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005735.3       ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005736.3       ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
                  *********** *****:**:** ***** *********** ** *********** ** 

HsCD00079036      CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005735.3       CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005736.3       CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
                  **.** ******.* ** ** *****:******** **.** ***.******* **.**.

HsCD00079036      AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005735.3       AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005736.3       AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
                  *****:***********.******** :*.******** *********** ***.* ** 

HsCD00079036      CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005735.3       CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005736.3       AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
                  ...** ******************** *****.** ***** ** ***** *********

HsCD00079036      ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005735.3       ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005736.3       ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
                  ** *****.******************** ** ***** **  *.*****..*:**.***

HsCD00079036      CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005735.3       CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005736.3       CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
                  *****....** **.**.** ***** *********** ***** ** ** *********

HsCD00079036      ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005735.3       ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005736.3       ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
                  *****.*****.***** ** *****:*****..* ** *****.***** **.** **:

HsCD00079036      GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005735.3       GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005736.3       GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
                  ***** **.***** *********************************** *********

HsCD00079036      ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005735.3       ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005736.3       ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
                  ***** .* ***** ** ***** ******** ****:*** **.*** : ***** ***

HsCD00079036      GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005735.3       GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005736.3       GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
                  **.**  : ******** :*.**  ***********.*****..*.*.**.*****..**

HsCD00079036      GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005735.3       GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005736.3       GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
                  ** ** *****.*****.*****.**.*********.* **.*****.*****.*  ***

HsCD00079036      TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005735.3       TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005736.3       TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
                  ***:.  ****:** ******** .**** .* ** *** *.************** ***

HsCD00079036      CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005735.3       CTGCTGTTCCAGCCGGACCTTGTCGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005736.3       TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
                   **** ***..***.**  * .* **.** ********.** .**** *****  ***  

HsCD00079036      TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005735.3       TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005736.3       TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
                  ********:** *****.************** ** ***** *** * ***** ** ***

HsCD00079036      TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005735.3       TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005736.3       TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
                  *****:*****:** ** ******** ** **:***.*. *.** **************.

HsCD00079036      CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005735.3       CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005736.3       CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
                  **:** *****.***.* **.****.***.**:**.** *****..*.***** *****.

HsCD00079036      TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005735.3       TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005736.3       TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
                  ******** *********** ***** ******** ***************** ******

HsCD00079036      AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTTG
NM_005735.3       AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTAG
NM_005736.3       AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
                  *****.*****.**.** **  ****: * ** ** .*.***** ****:.

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Adaptive Immune System
Reactome : Immune System
Reactome : MHC class II antigen presentation

Protein

SMART domain : ACTIN : Actin
UniProt : P42025
HPRD : 05508

Clone

Cloning Information : 226041
HIP Master Clone ID : 174147
Original Clone ID : FLH226041.01L

Organism


TAX_ID : 9606
Species Specific ID: 10120

Genome

Chromosome : 2
Map Location : 2q11.1-q11.2
Ensembl : ENSG00000115073

Reagents

Labome : ACTR1B-antibody