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DPF3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DPF3
Gene Name:  D4, zinc and double PHD fingers, family 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH258309.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079923

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1074nts         Open reading frame : 1 to 1074
Reference Sequence Alignment

ATGGCGACTGTCATTCACAACCCCCTGAAAGCGCTCGGGGACCAGTTCTACAAGGAAGCC
ATTGAGCACTGCCGGAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCC
TTCCTGGACTCACAGACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGG
CACCGAGGCCCAGGCCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGC
AAGAAGAGACGACTGCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCT
GAAGTGGAGCTTCCCCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCC
TTGCTCCGTGGCGAGGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAG
GAAATACAGAGGGTTTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGAT
TTGGAAGAGGATATTCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCACGGCTCTGCA
GGGGGCAGGAGGAGGCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGT
GACATCTGTGGCAAGCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACT
CACCTGGCCAGCGAGGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAAC
CACAGAAATGAGAACCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAAC
TACTGTGACTTCTGCTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAG
CTGGTGTCCTGCGCAGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAG
AAGGAGGCAGCGGCCGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGT
GACACGTCAACTTTCCGCGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGA
GGACGCCGCAGTGGCCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1087
Start on reference sequence 14
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DPF3
Symbol Nomenclature : DPF3
SYNONYM : BAF45C; CERD4
Designation : BRG1-associated factor 45C|zinc finger protein DPF3|zinc finger protein cer-d4
Full Nomenclature : D4, zinc and double PHD fingers, family 3
GENEID : 8110
HGNC : 17427
MIM : 601672
Vega : OTTHUMG00000171364
Target GenBank: BC060801

 

Reference Sequence Alignment

  

HsCD00079923        ------------------------------------------------------------
NM_001280543.1      ------------------------------------------------------------
NM_012074.4         ------------------------------------------------------------
NM_001280544.1      ATGTTTTATGGCAGAATAAATGGGCGTAACTTCGCCGCATCCTCGCTGCCGGTTGCTTTC
                                                                                

HsCD00079923        ------------------------------------------------------------
NM_001280543.1      ------------------------------------------------------------
NM_012074.4         ------------------------------------------------------------
NM_001280544.1      GCTGCAACACCGCTGATGCTGTTTCTACCGAACCCACAACTGATTTTCAGTTTCCCCATT
                                                                                

HsCD00079923        ---------------------------------------------ATGGCGACTGTCATT
NM_001280543.1      ----------------ATGGGGTTCACAGACCTGGAAGAGCCCATCTCTGGATGCCCTGG
NM_012074.4         ---------------------------------------------ATGGCGACTGTCATT
NM_001280544.1      TCCAGCCGAAATCACATAACCGGGCTGATGCCACCTGGTAAACTCAAGTTAGAGAACCTA
                                                                 .:   ..    *   

HsCD00079923        CACAACCCCCTGAAAGCG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_001280543.1      AGGCCCATGGGCTCTAGGGCTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_012074.4         CACAACCCCCTGAAAGCG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
NM_001280544.1      TTTCACATGTGCACCAGG-CTCGGGGACCAGTTCTACAAGGAAGCCATTGAGCACTGCCG
                       ..*.     :. . * *****************************************

HsCD00079923        GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_001280543.1      GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_012074.4         GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
NM_001280544.1      GAGTTACAACTCACGGCTGTGTGCAGAGCGCAGCGTGCGTCTTCCCTTCCTGGACTCACA
                    ************************************************************

HsCD00079923        GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_001280543.1      GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_012074.4         GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
NM_001280544.1      GACTGGGGTGGCCCAGAACAACTGCTACATCTGGATGGAGAAGAGGCACCGAGGCCCAGG
                    ************************************************************

HsCD00079923        CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGACT
NM_001280543.1      CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
NM_012074.4         CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
NM_001280544.1      CCTTGCCCCGGGCCAGCTGTATACATACCCTGCCCGCTGCTGGCGCAAGAAGAGACGATT
                    ********************************************************** *

HsCD00079923        GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_001280543.1      GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_012074.4         GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
NM_001280544.1      GCACCCACCTGAAGATCCAAAACTGCGGCTGCTGGAGATAAAACCTGAAGTGGAGCTTCC
                    ************************************************************

HsCD00079923        CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_001280543.1      CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_012074.4         CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
NM_001280544.1      CCTGAAGAAGGATGGGTTCACCTCAGAGAGCACCACGCTGGAAGCCTTGCTCCGTGGCGA
                    ************************************************************

HsCD00079923        GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_001280543.1      GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_012074.4         GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
NM_001280544.1      GGGGGTTGAGAAGAAGGTGGATGCCAGGGAGGAGGAAAGCATCCAGGAAATACAGAGGGT
                    ************************************************************

HsCD00079923        TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_001280543.1      TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_012074.4         TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
NM_001280544.1      TTTGGAAAATGATGAAAATGTAGAAGAAGGGAATGAAGAAGAGGATTTGGAAGAGGATAT
                    ************************************************************

HsCD00079923        TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCACGGCTCTGCAGGGGGCAGGAGGAG
NM_001280543.1      TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
NM_012074.4         TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
NM_001280544.1      TCCCAAGCGAAAGAACAGGACTAGAGGACGGGCTCGCGGCTCTGCAGGGGGCAGGAGGAG
                    ***********************************.************************

HsCD00079923        GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_001280543.1      GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_012074.4         GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
NM_001280544.1      GCACGACGCCGCCTCTCAGGAAGACCACGACAAACCTTACGTCTGTGACATCTGTGGCAA
                    ************************************************************

HsCD00079923        GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_001280543.1      GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_012074.4         GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
NM_001280544.1      GCGCTACAAGAACCGACCGGGGCTCAGCTACCACTATGCTCACACTCACCTGGCCAGCGA
                    ************************************************************

HsCD00079923        GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_001280543.1      GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_012074.4         GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
NM_001280544.1      GGAGGGGGATGAAGCTCAAGACCAGGAGACTCGGTCCCCACCCAACCACAGAAATGAGAA
                    ************************************************************

HsCD00079923        CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_001280543.1      CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_012074.4         CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
NM_001280544.1      CCACAGGCCCCAGAAAGGACCGGATGGAACAGTCATTCCCAATAACTACTGTGACTTCTG
                    ************************************************************

HsCD00079923        CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_001280543.1      CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_012074.4         CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
NM_001280544.1      CTTGGGGGGCTCCAACATGAACAAGAAGAGTGGGCGGCCTGAAGAGCTGGTGTCCTGCGC
                    ************************************************************

HsCD00079923        AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_001280543.1      AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_012074.4         AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
NM_001280544.1      AGACTGTGGACGCTCTGCTCATTTGGGAGGAGAAGGCAGGAAGGAGAAGGAGGCAGCGGC
                    ************************************************************

HsCD00079923        CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_001280543.1      CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_012074.4         CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
NM_001280544.1      CGCAGCACGTACCACGGAGGACTTATTCGGTTCCACGTCAGAAAGTGACACGTCAACTTT
                    ************************************************************

HsCD00079923        CCGCGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_001280543.1      CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_012074.4         CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
NM_001280544.1      CCACGGCTTTGATGAGGACGATTTGGAAGAGCCTCGCTCCTGTCGAGGACGCCGCAGTGG
                    **.*********************************************************

HsCD00079923        CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTTG
NM_001280543.1      CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
NM_012074.4         CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
NM_001280544.1      CCGGGGTTCGCCCACAGCAGATAAAAAGGGCAGTTGCTAA
                    **************************************:.

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q92784
HPRD : 03395

Clone

Cloning Information : 258309
HIP Master Clone ID : 194925
Original Clone ID : FLH258309.01L

Organism


TAX_ID : 9606
Species Specific ID: 8110

Genome

Chromosome : 14
Map Location : 14q24.2
Ensembl : ENSG00000205683

Reagents

Labome : DPF3-antibody