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HSPA9B (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HSPA9
Gene Name:  heat shock 70kDa protein 9 (mortalin)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH258747.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00080058

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2040nts         Open reading frame : 1 to 2040
Reference Sequence Alignment

ATGATAAGTGCCAGCCGAGCTGCAGCAGCCCGTCTCGTGGGCGCCGCAGCCTCCCGGGGC
CCTACGGCCGCCCGCCACCAGGATAGCTGGAATGGCCTTAGTCATGAGGCTTTTAGACTT
GTTTCAAGGCGGGATTATGCATCAGAAGCAATCAAGGGAGCAGTTGTTGGTATTGATTTG
GGTACTACCAACTCCTGCGTGGCAGTTATGGAAGGTAAACGAGCAAAGGTGCTGGAGAAT
GCCGAAGGTGCCAGAACCACCCCTTCAGTTGTGGCCTTTACAGCAGATGGTGAGCGACTT
GTTGGAATGCCGGCCAAGCGACAGGCTGTCACCAACCCAAACAATACATTTTATGCTACC
AAGCGTCTCATTGGCCGGCGATATGATGATCCTGAAGTACAGAAAGACATTAAAAATGTT
CCCTTTAAAATTGTCCGTGCCTCCAATGGTGATGCCTGGGTTGAGGCTCATGGGAAATTG
TATTCTCCGAGTCAGATTGGAGCATTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAAT
TACTTGGGGCGCACAGCAAAAAATGCTGTGATCACAGTCCCAGCTTATTTCAATGACTCG
CAGAGACAGGCCACTAAAGATGCTGGCCAGATATCTGGACTGAATGTGCTTCGGGTGATT
AATGAGCCCACAGCTGCTGCTCTTGCCTATGGTCTAGACAAATCAGAAGACAAAGTCATT
GCTGTATATGATTTAGGTGGTGGAACTTTTGATATTTCTATCCTGGAAATTCAGAAAGGA
GTATTTGAGGTGAAATCCACAAATGGGGATACCTTCTTAGGTGGGGAAGACTTTGACCAG
GCCTTGCTACGGCACATTGTGAAGGAGTTCAAGAGAGAGACAGGGGTTGATTTGACTAAA
GACAACATGGCACTTCAGAGGGTACGGGAAGCTGCTGAAAAGGCTAAGTGTGAACTCTCC
TCATCTGTGCAGACTGACATCAATTTGCCCTATCTTACAATGGATTCTTCTGGACCCAAG
CATTTGAATATGAAGTTGACCCGTGCTCAATTTGAAGGGATTGTCACTGATCTAATCAGA
AGGACTATCGCTCCATGCCAAAAAGCTATGCAAGATGCAGAAGTCAGCAAGAGTGACATA
GGAGAAGTGATTCTTGTGGGTGGCATGACTAGGATGCCCAAGGTTCAGCAGACTGTACAG
GATCTTTTTGGCAGAGCCCCAAGTAAAGCTGTCAATCCTGATGAGGCTGTGGCCATTGGA
GCTGCCATTCAGGGAGGTGTGTTGGCCGGCGATGTCACGGATGTGCTGCTCCTTGATGTC
ACTCCCCTGTCTCTGGGTATTGAAACTCTAGGAGGTGTCTTTACCAAACTTATTAATAGG
AATACCACTATTCCAACCAAGAAGAGCCAGGTATTCTCTACTGCCGCTGATGGTCAAACG
CAAGTGGAAATTAAAGTGTGTCAGGGTGAAAGAGAGATGGCTGGAGACAACAAACTCCTT
GGACAGTTTACTTTGATTGGAATTCCACCAGCCCCTCGTGGAGTTCCTCAGATTGAAGTT
ACATTTGACATTGATGCCAATGGGATAGTACATGTTTCTGCTAAAGATAAAGGCACAGGA
CGTGAGCAGCAGATTGTAATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAAT
ATGGTTAAAAATGCAGAGAAATATGCTGAAGAAGACCGGCGAAAGAAGGAACGAGTTGAA
GCAGTTAATATGGCTGAAGGAATCATTCACGACACAGAAACCAAGATGGAAGAATTCAAG
GACCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTTCCAAAATGAGGGAG
CTCCTGGCTAGAAAAGACAGCGAAACAGGAGAAAATATTAGACAGGCAGCATCCTCTCTT
CAGCAGGCATCATTGAAGCTGTTCGAAATGGCATACAAAAAGATGGCATCTGAGCGAGAA
GGCTCTGGAAGTTCTGGCACTGGGGAACAAAAGGAAGATCAAAAGGAGGAAAAACAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2091
Start on reference sequence 52
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HSPA9
Symbol Nomenclature : HSPA9
SYNONYM : CSA; GRP-75; GRP75; HSPA9B; MOT; MOT2; MTHSP75; PBP74
Designation : 75 kDa glucose-regulated protein|heat shock 70kD protein 9B|mortalin, perinuclear|mortalin-2|p66-mortalin|peptide-binding protein 74|stress-70 protein, mitochondrial
Full Nomenclature : heat shock 70kDa protein 9 (mortalin)
GENEID : 3313
HGNC : 5244
MIM : 600548
Vega : OTTHUMG00000129206
Target GenBank: BC000478

 

Reference Sequence Alignment

  

HsCD00080058      ATGATAAGTGCCAGCCGAGCTGCAGCAGCCCGTCTCGTGGGCGCCGCAGCCTCCCGGGGC
NM_004134.6       ATGATAAGTGCCAGCCGAGCTGCAGCAGCCCGTCTCGTGGGCGCCGCAGCCTCCCGGGGC
                  ************************************************************

HsCD00080058      CCTACGGCCGCCCGCCACCAGGATAGCTGGAATGGCCTTAGTCATGAGGCTTTTAGACTT
NM_004134.6       CCTACGGCCGCCCGCCACCAGGATAGCTGGAATGGCCTTAGTCATGAGGCTTTTAGACTT
                  ************************************************************

HsCD00080058      GTTTCAAGGCGGGATTATGCATCAGAAGCAATCAAGGGAGCAGTTGTTGGTATTGATTTG
NM_004134.6       GTTTCAAGGCGGGATTATGCATCAGAAGCAATCAAGGGAGCAGTTGTTGGTATTGATTTG
                  ************************************************************

HsCD00080058      GGTACTACCAACTCCTGCGTGGCAGTTATGGAAGGTAAACGAGCAAAGGTGCTGGAGAAT
NM_004134.6       GGTACTACCAACTCCTGCGTGGCAGTTATGGAAGGTAAACAAGCAAAGGTGCTGGAGAAT
                  ****************************************.*******************

HsCD00080058      GCCGAAGGTGCCAGAACCACCCCTTCAGTTGTGGCCTTTACAGCAGATGGTGAGCGACTT
NM_004134.6       GCCGAAGGTGCCAGAACCACCCCTTCAGTTGTGGCCTTTACAGCAGATGGTGAGCGACTT
                  ************************************************************

HsCD00080058      GTTGGAATGCCGGCCAAGCGACAGGCTGTCACCAACCCAAACAATACATTTTATGCTACC
NM_004134.6       GTTGGAATGCCGGCCAAGCGACAGGCTGTCACCAACCCAAACAATACATTTTATGCTACC
                  ************************************************************

HsCD00080058      AAGCGTCTCATTGGCCGGCGATATGATGATCCTGAAGTACAGAAAGACATTAAAAATGTT
NM_004134.6       AAGCGTCTCATTGGCCGGCGATATGATGATCCTGAAGTACAGAAAGACATTAAAAATGTT
                  ************************************************************

HsCD00080058      CCCTTTAAAATTGTCCGTGCCTCCAATGGTGATGCCTGGGTTGAGGCTCATGGGAAATTG
NM_004134.6       CCCTTTAAAATTGTCCGTGCCTCCAATGGTGATGCCTGGGTTGAGGCTCATGGGAAATTG
                  ************************************************************

HsCD00080058      TATTCTCCGAGTCAGATTGGAGCATTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAAT
NM_004134.6       TATTCTCCGAGTCAGATTGGAGCATTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAAT
                  ************************************************************

HsCD00080058      TACTTGGGGCGCACAGCAAAAAATGCTGTGATCACAGTCCCAGCTTATTTCAATGACTCG
NM_004134.6       TACTTGGGGCACACAGCAAAAAATGCTGTGATCACAGTCCCAGCTTATTTCAATGACTCG
                  **********.*************************************************

HsCD00080058      CAGAGACAGGCCACTAAAGATGCTGGCCAGATATCTGGACTGAATGTGCTTCGGGTGATT
NM_004134.6       CAGAGACAGGCCACTAAAGATGCTGGCCAGATATCTGGACTGAATGTGCTTCGGGTGATT
                  ************************************************************

HsCD00080058      AATGAGCCCACAGCTGCTGCTCTTGCCTATGGTCTAGACAAATCAGAAGACAAAGTCATT
NM_004134.6       AATGAGCCCACAGCTGCTGCTCTTGCCTATGGTCTAGACAAATCAGAAGACAAAGTCATT
                  ************************************************************

HsCD00080058      GCTGTATATGATTTAGGTGGTGGAACTTTTGATATTTCTATCCTGGAAATTCAGAAAGGA
NM_004134.6       GCTGTATATGATTTAGGTGGTGGAACTTTTGATATTTCTATCCTGGAAATTCAGAAAGGA
                  ************************************************************

HsCD00080058      GTATTTGAGGTGAAATCCACAAATGGGGATACCTTCTTAGGTGGGGAAGACTTTGACCAG
NM_004134.6       GTATTTGAGGTGAAATCCACAAATGGGGATACCTTCTTAGGTGGGGAAGACTTTGACCAG
                  ************************************************************

HsCD00080058      GCCTTGCTACGGCACATTGTGAAGGAGTTCAAGAGAGAGACAGGGGTTGATTTGACTAAA
NM_004134.6       GCCTTGCTACGGCACATTGTGAAGGAGTTCAAGAGAGAGACAGGGGTTGATTTGACTAAA
                  ************************************************************

HsCD00080058      GACAACATGGCACTTCAGAGGGTACGGGAAGCTGCTGAAAAGGCTAAGTGTGAACTCTCC
NM_004134.6       GACAACATGGCACTTCAGAGGGTACGGGAAGCTGCTGAAAAGGCTAAATGTGAACTCTCC
                  ***********************************************.************

HsCD00080058      TCATCTGTGCAGACTGACATCAATTTGCCCTATCTTACAATGGATTCTTCTGGACCCAAG
NM_004134.6       TCATCTGTGCAGACTGACATCAATTTGCCCTATCTTACAATGGATTCTTCTGGACCCAAG
                  ************************************************************

HsCD00080058      CATTTGAATATGAAGTTGACCCGTGCTCAATTTGAAGGGATTGTCACTGATCTAATCAGA
NM_004134.6       CATTTGAATATGAAGTTGACCCGTGCTCAATTTGAAGGGATTGTCACTGATCTAATCAGA
                  ************************************************************

HsCD00080058      AGGACTATCGCTCCATGCCAAAAAGCTATGCAAGATGCAGAAGTCAGCAAGAGTGACATA
NM_004134.6       AGGACTATCGCTCCATGCCAAAAAGCTATGCAAGATGCAGAAGTCAGCAAGAGTGACATA
                  ************************************************************

HsCD00080058      GGAGAAGTGATTCTTGTGGGTGGCATGACTAGGATGCCCAAGGTTCAGCAGACTGTACAG
NM_004134.6       GGAGAAGTGATTCTTGTGGGTGGCATGACTAGGATGCCCAAGGTTCAGCAGACTGTACAG
                  ************************************************************

HsCD00080058      GATCTTTTTGGCAGAGCCCCAAGTAAAGCTGTCAATCCTGATGAGGCTGTGGCCATTGGA
NM_004134.6       GATCTTTTTGGCAGAGCCCCAAGTAAAGCTGTCAATCCTGATGAGGCTGTGGCCATTGGA
                  ************************************************************

HsCD00080058      GCTGCCATTCAGGGAGGTGTGTTGGCCGGCGATGTCACGGATGTGCTGCTCCTTGATGTC
NM_004134.6       GCTGCCATTCAGGGAGGTGTGTTGGCCGGCGATGTCACGGATGTGCTGCTCCTTGATGTC
                  ************************************************************

HsCD00080058      ACTCCCCTGTCTCTGGGTATTGAAACTCTAGGAGGTGTCTTTACCAAACTTATTAATAGG
NM_004134.6       ACTCCCCTGTCTCTGGGTATTGAAACTCTAGGAGGTGTCTTTACCAAACTTATTAATAGG
                  ************************************************************

HsCD00080058      AATACCACTATTCCAACCAAGAAGAGCCAGGTATTCTCTACTGCCGCTGATGGTCAAACG
NM_004134.6       AATACCACTATTCCAACCAAGAAGAGCCAGGTATTCTCTACTGCCGCTGATGGTCAAACG
                  ************************************************************

HsCD00080058      CAAGTGGAAATTAAAGTGTGTCAGGGTGAAAGAGAGATGGCTGGAGACAACAAACTCCTT
NM_004134.6       CAAGTGGAAATTAAAGTGTGTCAGGGTGAAAGAGAGATGGCTGGAGACAACAAACTCCTT
                  ************************************************************

HsCD00080058      GGACAGTTTACTTTGATTGGAATTCCACCAGCCCCTCGTGGAGTTCCTCAGATTGAAGTT
NM_004134.6       GGACAGTTTACTTTGATTGGAATTCCACCAGCCCCTCGTGGAGTTCCTCAGATTGAAGTT
                  ************************************************************

HsCD00080058      ACATTTGACATTGATGCCAATGGGATAGTACATGTTTCTGCTAAAGATAAAGGCACAGGA
NM_004134.6       ACATTTGACATTGATGCCAATGGGATAGTACATGTTTCTGCTAAAGATAAAGGCACAGGA
                  ************************************************************

HsCD00080058      CGTGAGCAGCAGATTGTAATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAAT
NM_004134.6       CGTGAGCAGCAGATTGTAATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAAT
                  ************************************************************

HsCD00080058      ATGGTTAAAAATGCAGAGAAATATGCTGAAGAAGACCGGCGAAAGAAGGAACGAGTTGAA
NM_004134.6       ATGGTTAAAAATGCAGAGAAATATGCTGAAGAAGACCGGCGAAAGAAGGAACGAGTTGAA
                  ************************************************************

HsCD00080058      GCAGTTAATATGGCTGAAGGAATCATTCACGACACAGAAACCAAGATGGAAGAATTCAAG
NM_004134.6       GCAGTTAATATGGCTGAAGGAATCATTCACGACACAGAAACCAAGATGGAAGAATTCAAG
                  ************************************************************

HsCD00080058      GACCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTTCCAAAATGAGGGAG
NM_004134.6       GACCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTTCCAAAATGAGGGAG
                  ************************************************************

HsCD00080058      CTCCTGGCTAGAAAAGACAGCGAAACAGGAGAAAATATTAGACAGGCAGCATCCTCTCTT
NM_004134.6       CTCCTGGCTAGAAAAGACAGCGAAACAGGAGAAAATATTAGACAGGCAGCATCCTCTCTT
                  ************************************************************

HsCD00080058      CAGCAGGCATCATTGAAGCTGTTCGAAATGGCATACAAAAAGATGGCATCTGAGCGAGAA
NM_004134.6       CAGCAGGCATCACTGAAGCTGTTCGAAATGGCATACAAAAAGATGGCATCTGAGCGAGAA
                  ************ ***********************************************

HsCD00080058      GGCTCTGGAAGTTCTGGCACTGGGGAACAAAAGGAAGATCAAAAGGAGGAAAAACAGTTG
NM_004134.6       GGCTCTGGAAGTTCTGGCACTGGGGAACAAAAGGAAGATCAAAAGGAGGAAAAACAGTAA
                  **********************************************************:.

Pathways

Panther : Parkinson disease
Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

UniProt : P38646
HPRD : 02770

Clone

Cloning Information : 258747
HIP Master Clone ID : 195270
Original Clone ID : FLH258747.01L

Organism


TAX_ID : 9606
Species Specific ID: 3313

Genome

Chromosome : 5
Map Location : 5q31.1
Ensembl : ENSG00000113013

Reagents

Labome : HSPA9-antibody