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ADM2 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ADM2
Gene Name:  adrenomedullin 2
Sequence             
Original Clone ID: FLH264744.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00080125

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 447nts         Open reading frame : 1 to 447
Reference Sequence Alignment

ATGGCCCGGATCCCGACGGCCGCCCTGGGTTGCATCAGCCTCCTCTGCCTGCAGCTCCCT
GGCTCGCTGTCCCGCAGCCTGGGCGGGGACCCGCGACCCGTCAAACCCAGGGAGCCCCCA
GCCCGGAGCCCTTCCAGCAGCCTGCAGCCCAGGCACCCCGCACCCCGACCTGTGGTCTGG
AAGCTTCACCGGGCCCTCCAGGCACAGAGGGGTGCCGGCCTGGCCCCTGTTATGGGTCAG
CCTCTCCGGGATGGTGGCCGCCAACACTCGGGCCCCCGAAGACACTCGGGCCCCCGCAGG
ACCCAAGCCCAGCTCCTGCGAGTGGGCTGTGTGCTGGGCACCTGCCAGGTGCAGAATCTC
AGCCACCGCCTGTGGCAACTCATGGGACCGGCCGGCCGGCAGGACTCAGCTCCTGTGGAC
CCCAGCAGCCCCCACAGCTATGGCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 712
Start on reference sequence 266
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ADM2
Symbol Nomenclature : ADM2
SYNONYM : AM2; dJ579N16.4
Designation : intermedin
Full Nomenclature : adrenomedullin 2
GENEID : 79924
Locus Tag : RP4-579N16.10-002
GI : 148921709
GenBank Accession : BC146569
HGNC : 28898
MIM : 608682
Vega : OTTHUMG00000150202
Target GenBank: NM_024866

 

Reference Sequence Alignment

  

HsCD00080125        ATGGCCCGGATCCCGACGGCCGCCCTGGGTTGCATCAGCCTCCTCTGCCTGCAGCTCCCT
NM_024866.5         ATGGCCCGGATCCCGACGGCCGCCCTGGGTTGCATCAGCCTCCTCTGCCTGCAGCTCCCT
NM_001253845.1      ATGGCCCGGATCCCGACGGCCGCCCTGGGTTGCATCAGCCTCCTCTGCCTGCAGCTCCCT
                    ************************************************************

HsCD00080125        GGCTCGCTGTCCCGCAGCCTGGGCGGGGACCCGCGACCCGTCAAACCCAGGGAGCCCCCA
NM_024866.5         GGCTCGCTGTCCCGCAGCCTGGGCGGGGACCCGCGACCCGTCAAACCCAGGGAGCCCCCA
NM_001253845.1      GGCTCGCTGTCCCGCAGCCTGGGCGGGGACCCGCGACCCGTCAAACCCAGGGAGCCCCCA
                    ************************************************************

HsCD00080125        GCCCGGAGCCCTTCCAGCAGCCTGCAGCCCAGGCACCCCGCACCCCGACCTGTGGTCTGG
NM_024866.5         GCCCGGAGCCCTTCCAGCAGCCTGCAGCCCAGGCACCCCGCACCCCGACCTGTGGTCTGG
NM_001253845.1      GCCCGGAGCCCTTCCAGCAGCCTGCAGCCCAGGCACCCCGCACCCCGACCTGTGGTCTGG
                    ************************************************************

HsCD00080125        AAGCTTCACCGGGCCCTCCAGGCACAGAGGGGTGCCGGCCTGGCCCCTGTTATGGGTCAG
NM_024866.5         AAGCTTCACCGGGCCCTCCAGGCACAGAGGGGTGCCGGCCTGGCCCCTGTTATGGGTCAG
NM_001253845.1      AAGCTTCACCGGGCCCTCCAGGCACAGAGGGGTGCCGGCCTGGCCCCTGTTATGGGTCAG
                    ************************************************************

HsCD00080125        CCTCTCCGGGATGGTGGCCGCCAACACTCGGGCCCCCGAAGACACTCGGGCCCCCGCAGG
NM_024866.5         CCTCTCCGGGATGGTGGCCGCCAACACTCGGGCCCCCGAAGACACTCGGGCCCCCGCAGG
NM_001253845.1      CCTCTCCGGGATGGTGGCCGCCAACACTCGGGCCCCCGAAGACACTCGGGCCCCCGCAGG
                    ************************************************************

HsCD00080125        ACCCAAGCCCAGCTCCTGCGAGTGGGCTGTGTGCTGGGCACCTGCCAGGTGCAGAATCTC
NM_024866.5         ACCCAAGCCCAGCTCCTGCGAGTGGGCTGTGTGCTGGGCACCTGCCAGGTGCAGAATCTC
NM_001253845.1      ACCCAAGCCCAGCTCCTGCGAGTGGGCTGTGTGCTGGGCACCTGCCAGGTGCAGAATCTC
                    ************************************************************

HsCD00080125        AGCCACCGCCTGTGGCAACTCATGGGACCGGCCGGCCGGCAGGACTCAGCTCCTGTGGAC
NM_024866.5         AGCCACCGCCTGTGGCAACTCATGGGACCGGCCGGCCGGCAGGACTCAGCTCCTGTGGAC
NM_001253845.1      AGCCACCGCCTGTGGCAACTCATGGGACCGGCCGGCCGGCAGGACTCAGCTCCTGTGGAC
                    ************************************************************

HsCD00080125        CCCAGCAGCCCCCACAGCTATGGCTAA
NM_024866.5         CCCAGCAGCCCCCACAGCTATGGCTGA
NM_001253845.1      CCCAGCAGCCCCCACAGCTATGGCTGA
                    *************************.*

Pathways

Reactome : Calcitonin-like ligand receptors
Reactome : Class B/2 (Secretin family receptors)
Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

UniProt : Q7Z4H4
HPRD : 10563

Clone

Cloning Information : 264744
HIP Master Clone ID : 196842
IMAGE ID : 100014799
Original Clone ID : FLH264744.01X

Organism


TAX_ID : 9606
Species Specific ID: 79924

Genome

Chromosome : 22
Map Location : 22q13.33
Ensembl : ENSG00000128165

Reagents

Labome : ADM2-antibody