DNASU Plasmid Repository • 480.965.5697 | Email

C20orf186 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  BPIFB4
Gene Name:  BPI fold containing family B, member 4
Sequence             
Original Clone ID: FLH264775.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00080156

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1728nts         Open reading frame : 1 to 1728
Reference Sequence Alignment

ATGCTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGT
GATATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAA
AAACTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTG
GGGGGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGA
AACAGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATAC
AGGGCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCT
GGAGTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCA
GCTGATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGAT
GGAGGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGC
ACTGTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTG
CGGCTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGG
AAGAGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGG
CTGACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTA
GGGGGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTG
AACCGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTG
GGTCTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGA
AGTGTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGAC
CTCAACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCA
GCTGTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAG
CTGGCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCT
CTAGACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACA
CTGGGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATC
AGGATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAG
GTGTTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGC
CTCATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATG
ATTGATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTT
GATATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCA
ATGAACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGC
AATGCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1728
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB4
Symbol Nomenclature : BPIFB4
SYNONYM : C20orf186; LPLUNC4; RY2G5; dJ726C3.5
Designation : BPI fold-containing family B member 4|ligand-binding protein RY2G5|likely ortholog of rat probable ligand-binding protein RY2G5|long palate, lung and nasal epithelium carcinoma associated 4|long palate, lung and nasal epithelium carcinoma-associated protein 4
Full Nomenclature : BPI fold containing family B, member 4
GENEID : 149954
GI : 148922356
GenBank Accession : BC146505
HGNC : 16179
Vega : OTTHUMG00000032235
Target GenBank: NM_182519

 

Reference Sequence Alignment

  

HsCD00080156      ------------------------------------------------------------
NM_182519.2       ATGTGGATGGCCTGGTGTGTGGCTGCGCTGTCTGTGGTGGCTGTGTGTGGCACCAGCCAC
                                                                              

HsCD00080156      ---------------------------------------------------------ATG
NM_182519.2       GAGACAAACACGGTCCTCAGGGTGACGAAAGATGTGTTGAGCAATGCCATTTCAGGCATG
                                                                           ***

HsCD00080156      CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
NM_182519.2       CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
                  ************************************************************

HsCD00080156      ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
NM_182519.2       ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
                  ************************************************************

HsCD00080156      CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
NM_182519.2       CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
                  ************************************************************

HsCD00080156      GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
NM_182519.2       GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
                  ************************************************************

HsCD00080156      AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
NM_182519.2       AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
                  ************************************************************

HsCD00080156      GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
NM_182519.2       GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
                  ************************************************************

HsCD00080156      GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
NM_182519.2       GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
                  ************************************************************

HsCD00080156      GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
NM_182519.2       GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
                  ************************************************************

HsCD00080156      GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
NM_182519.2       GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
                  ************************************************************

HsCD00080156      GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
NM_182519.2       GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
                  ************************************************************

HsCD00080156      CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
NM_182519.2       CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
                  ************************************************************

HsCD00080156      AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
NM_182519.2       AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
                  ************************************************************

HsCD00080156      ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
NM_182519.2       ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
                  ************************************************************

HsCD00080156      GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
NM_182519.2       GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
                  ************************************************************

HsCD00080156      CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
NM_182519.2       CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
                  ************************************************************

HsCD00080156      CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
NM_182519.2       CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
                  ************************************************************

HsCD00080156      GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
NM_182519.2       GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
                  ************************************************************

HsCD00080156      AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
NM_182519.2       AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
                  ************************************************************

HsCD00080156      GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
NM_182519.2       GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
                  ************************************************************

HsCD00080156      GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
NM_182519.2       GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
                  ************************************************************

HsCD00080156      GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
NM_182519.2       GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
                  ************************************************************

HsCD00080156      GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
NM_182519.2       GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
                  ************************************************************

HsCD00080156      ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
NM_182519.2       ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
                  ************************************************************

HsCD00080156      TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
NM_182519.2       TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
                  ************************************************************

HsCD00080156      ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
NM_182519.2       ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
                  ************************************************************

HsCD00080156      GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
NM_182519.2       GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
                  ************************************************************

HsCD00080156      ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
NM_182519.2       ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
                  ************************************************************

HsCD00080156      AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
NM_182519.2       AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
                  ************************************************************

HsCD00080156      GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCATAA
NM_182519.2       GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCATGA
                  *******************************************.*

Protein

SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
UniProt : P59827
HPRD : 16645

Clone

Cloning Information : 264775
HIP Master Clone ID : 196873
IMAGE ID : 100015034
Original Clone ID : FLH264775.01X

Organism


TAX_ID : 9606
Species Specific ID: 149954

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186191

Reagents

Labome : BPIFB4-antibody