Gene:
Gene Symbol:
TAF2
Gene Name:
TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
Original Clone ID:
FLH264922.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00080302
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCGCTGACTGGTGTAGAGCCCGCCAGAATGAACAGGAAGAAAGGAGACAAGGGCTTT
GAAAGCCCAAGGCCATATAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAATTTC
CAGAGAAAATCTGTTGTGGGATTTGTGGAACTGACTATATTTCCCACAGTTGCAAACTTG
AATAGAATCAAGTTGAACAGCAAACAGTGTAGAATATACCGAGTAAGGATCAATGATTTA
GAGGCTGCTTTTATTTATAATGACCCAACCTTGGAAGTTTGTCACAGTGAATCAAAACAG
AGAAACCTCAATTATTTTTCCAATGCTTATGCAGCTGCAGTTAGTGCTGTGGACCCTGAT
GCAGGAAATGGAGAACTTTGCATTAAGGTTCCATCAGAGCTATGGAAACACGTTGATGAG
TTAAAGGTCCTGAAGATACACATCAATTTTTCTTTGGATCAGCCCAAAGGAGGTCTTCAT
TTTGTGGTACCCAGTGTAGAGGGAAGTATGGCAGAGAGAGGTGCTCATGTTTTCTCTTGT
GGGTATCAAAATTCTACAAGATTTTGGTTCCCTTGTGTTGATTCATACTCTGAATTGTGT
ACATGGAAATTAGAATTTACAGTAGATGCTGCAATGGTTGCTGTTTCTAATGGCGATTTG
GTGGAGACAGTGTATACTCATGATATGAGGAAGAAAACTTTCCATTATATGCTTACCATT
CCTACAGCAGCGTCAAATATCTCCTTGGCCATTGGACCATTTGAAATACTGGTAGATCCA
TACATGCATGAGGTTACTCATTTTTGTTTGCCCCAACTTCTTCCATTGCTGAAACATACC
ACATCATACCTTCATGAAGTCTTTGAATTTTATGAAGAAATTCTTACATGTCGTTACCCA
TACTCCTGTTTTAAGACTGTCTTCATTGATGAGGCTTATGTTGAAGTGGCTGCTTATGCT
TCCATGAGCATTTTTAGCACAAATCTTTTACACAGTGCCATGATTATAGATGAGACACCT
TTGACTAGAAGGTGTTTAGCCCAATCCTTGGCCCAGCAGTTTTTTGGTTGTTTCATATCT
AGAATGTCTTGGTCTGATGAATGGGTGCTGAAGGGAATTTCAGGCTATATCTATGGACTT
TGGATGAAAAAAACTTTTGGTGTTAATGAGTACCGCCATTGGATTAAAGAGGAGCTAGAC
AAAATAGTGGCATATGAACTAAAAACTGGTGGGGTTTTACTACATCCCATATTTGGTGGA
GGAAAAGAGAAGGATAATCCGGCTTCCCATCTACACTTTTCAATAAAGCATCCACATACA
CTGTCCTGGGAATACTACACTATGTTTCAGTGTAAAGCCCACCTTGTGATGAGATTGATT
GAAAATAGGATCAGTATGGAATTTATGCTACAAGTTTTCAATAAACTGCTAAGTCTGGCT
AGTACTGCTTCATCTCAGAAGTTCCAGTCACATATGTGGAGTCAGATGTTGGTTTCCACA
TCTGGGTTTTTGAAATCCATTTCAAATGTCTCTGGCAAAGATATTCAGCCGTTAATAAAG
CAGTGGGTAGATCAGAGTGGAGTGGTAAAATTTTATGGAAGTTTTGCATTTAATAGAAAA
CGAAATGTCTTGGAACTGGAAATAAAACAGGACTATACATCTCCTGGAACTCAGAAATAC
GTGGGACCACTTAAAGTGACAGTGCAGGAGTTAGATGGATCCTTCAATCATACACTGCAA
ATTGAAGAAAACAGCCTTAAACATGATATACCCTGCCATTCCAAAAGTAGAAGGAATAAA
AAGAAAAAAATCCCACTGATGAATGGAGAAGAAGTTGATATGGATCTTTCTGCAATGGAT
GCTGATTCCCCTTTGCTGTGGATAAGGATAGACCCAGATATGTCAGTATTGAGGAAGGTA
GAATTTGAGCAAGCTGATTTTATGTGGCAGTATCAGCTCCGCTATGAGAGAGATGTTGTT
GCACAGCAGGAATCCATTTTGGCTTTGGAAAAATTCCCTACTCCAGCATCTCGGCTTGCA
CTCACTGATATATTAGAACAAGAGCAGTGTTTCTACAGAGTAAGAATGTCAGCTTGCTTC
TGTCTTGCAAAGATTGCAAATTCAATGGTGAGCACATGGACAGGACCACCAGCCATGAAG
TCACTCTTCACTAGGATGTTTTGTTGTAAAAGTTGTCCAAACATTGTGAAAACAAACAAC
TTTATGAGCTTTCAAAGCTATTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTATTA
AGAGATGTTCATAATCTTTGTCCTAAAGAAGTCTTAACATTTATTTTAGACTTAATCAAG
TACAATGACAACAGGAAAAATAAGTTTTCAGATAACTATTATCGTGCAGAAATGATTGAT
GCCCTGGCCAACTCTGTTACACCTGCAGTCAGTGTGAATAATGAAGTTAGAACTTTGGAT
AACTTAAATCCTGATGTGCGACTCATTCTTGAAGAAATCACCAGATTTTTGAATATGGAA
AAACTTCTTCCGAGTTACAGGCATACCATCACTGTCAGTTGTTTGAGAGCCATACGGGTA
CTTCAGAAGAACGGACATGTGCCAAGTGATCCAGCTCTTTTTAAATCTTATGCTGAATAT
GGCCACTTTGTGGACATTAGGATAGCAGCTTTGGAAGCAGTTGTTGATTATACTAAAGTG
GACAGAAGTTATGAAGAACTGCAATGGCTACTTAATATGATTCAGAATGACCCTGTACCC
TATGTAAGGCATAAGATTCTCAACATGTTGACTAAGAACCCACCATTTACTAAGAACATG
GAGTCTCCCTTATGCAATGAAGCCCTGGTAGATCAACTTTGGAAACTTATGAATTCTGGT
ACTTCACATGACTGGAGGTTACGGTGTGGTGCTGTGGACTTGTACTTCACACTTTTTGGC
CTCAGTAGACCTTCCTGTTTACCCTTGCCAGAGCTTGGGTTGGTTCTTAATCTAAAGGAG
AAAAAAGCTGTCTTGAATCCTACCATAATTCCAGAGTCAGTAGCAGGCAACCAAGAAGCT
GCAAATAATCCAAGCAGTCACCCACAGCTAGTTGGATTTCAGAACCCTTTTTCCAGTTCT
CAAGATGAGGAGGAGATTGATATGGATACTGTTCATGATAGCCAGGCCTTCATTTCCCAT
CATTTAAACATGCTTGAAAGGCCGTCAACTCCAGGGCTCTCGAAATATCGGCCAGCTAGC
TCCCGATCTGCTTTAATACCCCAGCACTCAGCAGGCTGTGACAGCACACCCACCACAAAA
CCCCAGTGGAGTTTGGAACTTGCACGGAAGGGAACAGGTAAAGAACAAGCACCTTTGGAG
ATGAGTATGCATCCAGCGGCAAGCGCTCCACTCTCAGTCTTTACTAAGGAATCTACAGCC
TCCAAACACAGTGACCACCATCACCACCATCACCATGAGCACAAGAAAAAGAAGAAGAAG
CATAAACATAAGCACAAACACAAGCATAAGCATGACAGTAAAGAAAAGGACAAGGAGCCT
TTCACTTTCTCCAGCCCTGCCAGTGGCAGGTCTATTCGTTCTCCTTCCCTTTCAGAC
Protein sequence predicted based on nucleotide sequence
MPLTGVEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANL NRIKLNSKQCRIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPD AGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSC GYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTI PTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYP YSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFIS RMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGG GKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLA STASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRK RNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNK KKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVV AQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMK SLFTRMFCCKSCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIK YNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME KLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKV DRSYEELQWLLNMIQNDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSG TSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPESVAGNQEA ANNPSSHPQLVGFQNPFSSSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPAS SRSALIPQHSAGCDSTPTTKPQWSLELARKGTGKEQAPLEMSMHPAASAPLSVFTKESTA SKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTFSSPASGRSIRSPSLSD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
271
End on reference sequence
3870
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
