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Zfp352 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Zfp352
Gene Name:  zinc finger protein 352
Sequence             
Original Clone ID: FLH265356.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00080734

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1647nts         Open reading frame : 1 to 1647

ATGGCAGAAAACACAGGTGCTTCCCAGCCAGGACCCTGCAATACACAGCCTTCCCCTGTG
GAGTCCAGCCAGCTTTTCTCAGGTCAGAGCTCAGAGATGCTTACCTGGAAACAAACAGTG
ATGGAATCTGCATCCTGTAGCACACAGGAGACAACCTGTACTCAGAACTCAACAGCATAC
CCAGGAAAAGCCATTGGTTTCCATTTTGGACAGCCATTTGAGAACACTTCTTGGAACCAG
CAAGTTGTGTCTGCTGAGCAGAAAATATCTCCTGCAGGTCTTTACCATACACAGCTCAAC
AGCGCCCCTCAGATATGCCTTCCTCCCCAGAAGACAACAAGAGTTGACACCAGGGAGACT
TTCTACAATGAAGACTACTGGATGAGGACACTGAACTCTGACAAGACTCTCTTGACCCAA
AATACAGCCATGTGTGAGAATCAGAGGGTTACCTTTGATAATCATCAAATTGCTCTCTAT
GGAAGCCATGCTGGCCAGGACCCAAATGTGGGTCACGCCTTTGCTTCTGAACATGACTGG
ACCTCCTACAACAGTCATATGACAACCAGGAATGGAGGGCATCTGACTCTCTATGAGAGT
CAGATGACAGCACCCAGTTGTAGCCAGACCCTTCATCCCAATCAAATCATAACATCCTTT
TCTGAACAGAATTATTTTGAGGATCAGCAGAGTAACCTAGCAGTTAATAATGGATTCTAT
GGAAATCAGGTGATATTGCCAAATGGATACCAGGCTTTCTATGAGCCTCAGATGAGAGCT
AACTTTGACCAAACAAATGACCAAATGAAATCCTTCAGTGGTCAGAATGTCTGCAAAGGT
CAGGAGAATCTCCTCAGTGGTGAGTGCTCCCTCTCTGGTTATCAGACATCAGGCTACAGA
TGTGACCAGGATTTGATTGTGAATCCTCAGGTGACAAGTCCAATTGGTGGCCAGCCTCTC
TCTGACTTTCAGACACAAACCTCAAGTTTTGATACAGCTCTTCCTGGGTCTAAGAGGACA
AGACCCAGTGCAGAAGACAACCTGGCTTGCCCTTTGGAGACAAGTCCCACATCTGAAGAA
ACACTCTACTTGGGTCAGATGAGGACCTCTGTTGATCAAAATTTCTACTCAAGTCAGAAT
GGAACACCTAATATTAAAGAAAGCCTTGATCCTCAGGTGACTTCATTTAGTTTGCAAGCT
CCATATGTGGGTGAATCCTCATACCCTTCATGTTCCCCTTTGATTCAAAAACAACCTCAG
AAAAACTCTTCGGCTTCCAGTTTAGTCCAGAGACAGCATCCAGAGATGAAGTTAGACTTC
AAGACCCAGAGCCCTGTGTCACAGAAGGACCCTAGTCCTTTGAAGCTCTACTTTTGTACT
TATAAGGGTTGTACGAAATTTTACAAAAGAGCTTACCACCTCAAAGAACACCAGAAGAAA
CACACTGATAAGAGGAAATACGGGTGTGATGAGCCTGGGTGCACATGGTCGTTTTTCCGC
CTATGTGATCTCAACAGACACAAAGAAAAGCACAATGGTGAGAGGTTCTACGCTTGTCCC
TTGTGCTCTACAAACTATTCCAGACTGCTTTATCTCAAAAAGCATCTGGAGAAGAAACAT
GCTCAAGCACAACCCACCACTGCAACC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1854
Start on reference sequence 205
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Zfp352
Symbol Nomenclature : Zfp352
SYNONYM : 2czf48
Full Nomenclature : zinc finger protein 352
GENEID : 236537
Locus Tag : RP23-354E5.1
GI : 147898090
GenBank Accession : BC140425
Mouse Genome Informatics : 2387418
Vega : OTTMUSG00000007775
Target GenBank: NM_153102

 

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : A2AML7

Clone

Cloning Information : 265356
HIP Master Clone ID : 197454
IMAGE ID : 100014466
Original Clone ID : FLH265356.01F

Organism


TAX_ID : 10090
Species Specific ID: 236537

Genome

Chromosome : 4
Map Location : 4 C5|4
Ensembl : ENSMUSG00000070902

Reagents

Labome : Zfp352-siRNA