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Nlrp9c (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nlrp9c
Gene Name:  NLR family, pyrin domain containing 9C
Sequence             
Original Clone ID: FLH265575.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00080952

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3012nts         Open reading frame : 1 to 3012

ATGGTTGACTCATCTAGCTATGGCTTGCTGCAGTATTTTCAAAAACTCAGTGATGAAGAG
TTCCAGAGATTTAAAGAGCTTCTCCAGAAAGAGCAAGAGAAATTTAAACTCAAACCACTC
TCCTGGACTAAGATTAAGAACACTTCCAAAGAAGACCTGGTAACGCAATTGTACACACAT
TATCCAAGACAGGTATGGGATATGGTGTTGAACCTGTTTCTACAGGTCAATAGGAAAGAC
CTCTCAACCATGGCTCAGATAGAAAGGAGAGATAAACAAAATAAATACAAAGAGTTTATG
AAGAATTTATTTCAATACATATGGACATCTGAGACCAACACTTATATGCCTGATAGAAGC
TACAACACCATTATAGATCGTCAATACAAAGCATTACTGGACATATTTGATTCTGAGTCA
GACCCAGCTACTGCAGTTGTATTAGGTACTAGAGGAAAAGGAAAAACAGTTTTTTTAAGA
AAAGCAATGTTGGATTGGGCATCAGGAGTTTTACTGCAGAACCGGTTTCAGTATGTTTTC
TTCTTCTCTGTCTTCTCACTTAACAATACCACAGAGTTAAGCTTAGCTGAGCTTATCTCA
AGTAAATTGCCTGAGTGTTCTGAGACACTGGATGACATTTTATCCAATCCAAAGAGAATC
TTGTTTGTTCTGGATGGATTTGATTACCTGAAATTTGACTTGGAACTCAGGACAAACTTG
TGCAATGATTGGAGGAAGAGGCTGCCAACACAAAATGTTTTGAGCAGTTTGCTACAGAAA
ATAATGCTCCCAGAGTGCTCTCTGCTTCTTGAATTGGGAGAGTCAAGTTGTTCAAAAATT
ATTCCTTTGCTGCAGAATCCTAGGGAGATCATTATGTCAGGATTATCTGAACAGTCTATA
TATTTCTACTGTGTGTCCTTCTTCAAAATTCAGTTGGGTGTAGAAGTCTTTAAGGATTTA
AAGAAAAACGAACCATTGTTTACTTTATGCAGCAATCCTAGTATGTTGTGGATGATCTGT
AGTTCTTTAATGTGGGGGCATTATTCGAGAGAGGAAGTAATCTCTTCCAGTGAATCAACA
TCAGCTATCCACACGATATTTATCATGAGTGCATTCAAATCAATATTTGGCTTGGGCTCA
TCTAAATACAAGAGGTTCAAATTAAAGACCCTGTGCACCTTGGCTGTAGAGGGAATGTGG
AAACAGGTATATGTGTTTGACTCTGAGGACCTCAGGAGGAATAAGATATCTGAATCTGAT
AAGACAGTGTGGCTGAAAATGAAATTTCTGCAAATTCAAGGTAACAACATTATGTTTTAC
CATTCTACACTACAGTGGTACTTTGCTACCCTGTTCTATTTTCTCAAACAGGACAAAGAC
ACATATCACCCAGTTATTGGAAGCCTACCTCAACTTTTAGGAGAAATATATGCTCATAAA
CAGAACCAATGGACCCATGCACAGACATTTTTCTTTGGAATTGCCACTAAACATGTGATC
ACTTTGCTTAAACCATGCTTTGGCAATATATCATTCAAAACAATAAGACAGGAAATCATT
AGATACCTTAAAAGTCTCAGTCAACCAGAGTGTAATGAGAAACTGGTGCATCCCAAGAAG
TTGTTCTTCTGTCTAATTGAAAACCAGGAGGAAAGATTTGTAAGTCAAGTGATGAATCTA
TTTGAAGAAATTACTGTTGATATTAGTGATAGTGATGATTTGGGAGCAGCTGAATACAGT
CTGCTGCGAGCCTCAAAATTGAAAAACCTTCACCTGCATATACAAAAGAAAGTTTTTTCA
GAAATCCATGATCCCGAATATGGTAGCTTAGAAAACTTTAAACTGGACCAAAAATTTTCA
GCCATCAATTGGACTATGTTATCCATTCTTTTCTGTAACTTACATGTGTTGGATCTGGGC
AGCTGTCATTTCAATAAAAAGGTCATAGAGGTCCTCTGTAATTCTTTGTCTCCAACTCCT
AATATGCCTCTGACTGTTTTTAAGCTCCAAAGATTGTTGTGTTCCTTCATGACTAACTTT
GGAGATGGTTCATTATTTTGCACATTTCTTCAGATACCACAACTGAAATACCTGAACCTG
TATGGCACTGATCTCTCTAATGATGTGGTTGAGATGCTGTGCTCAGCTCTGAAATGTTCA
ACATGTCGAGTGGAGGAGCTGTTGTTGGGAAAATGTGACATATCAAGTGAGGCTTGTGGC
ATAATGGCAACTTTCCTTATCAACTCTAAGGTGAAACATCTCTCATTGGTTGAAAATCCC
TTGAAGAACAAAGGAGTGATGTTTCTGTGTAAAATGCTGAAGGACCCAAGCTGTGTTCTG
GAATCACTGATGTTGTCTTACTGCTGTCTCACATTCATTGCCTGTGGACATCTCTATGAA
GCTCTTTTAAGCAACAAACACCTCTCTCTCCTTGATCTGGGATCAAATTTCCTGGAAGAT
ATTGGAGTGAACCTTCTGTGTGAAGCTTTGAAATATCCAAACTGCACCCTCAAGGAGTTA
TGGTTGCCTGGTTGCTATTTAACTTCAGAATGCTGTGAAGAGATCTCGGCTGTCCTGACT
TGCAATAAAAATCTAAAGACCCTGAAACTTGGCAACAATAACATACAAGACACTGGTGTC
AAACGGTTATGTGAAGCTCTGTGTCATCCTAAATGTAAAGTGCAGTGTCTTGGGTTAGAC
ATGTGTGAGCTCAGCAATGACTGTTGTGAAGACCTGGCATTGGCTCTTATCACTTGCAAC
ACATTGAAGAGCTTAAATCTGGACTGGAATGCCTTGCATCACTCTGGGCTGGTGATGCTC
TGTGAGGCTCTGAACCACAAAAAGTGTAAACTGAATATGCTTGGACTGGACAAATCTTCA
TTTTCTGAGGAATCACAGACATTTCTGCAAGCTGTGGAAAAGAAAAATAACAATCTGAAT
GTTTTGCATTTTCCCTGGGTTGAAGATGAACTCAAAAAGAGGGGGGTTCGTCTGGTATGG
AACAGCAAGAAT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3020
Start on reference sequence 6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nlrp9c
Symbol Nomenclature : Nlrp9c
SYNONYM : AA636537; BB062542; Nalp-zeta; Nalp9c
Designation : NACHT, LRR and PYD containing protein 9c|NACHT, LRR and PYD domains-containing protein 9C
Full Nomenclature : NLR family, pyrin domain containing 9C
GENEID : 330490
GI : 151556659
GenBank Accession : BC148455
Mouse Genome Informatics : 3028627
Vega : OTTMUSG00000033294
Target GenBank: NM_001042612

 

Clone

Cloning Information : 265575
HIP Master Clone ID : 197673
IMAGE ID : 100015438
Original Clone ID : FLH265575.01F

Organism


TAX_ID : 10090
Species Specific ID: 330490

Genome

Chromosome : 7
Map Location : 7 A3|7
Ensembl : ENSMUSG00000040614

Reagents

Labome : Nlrp9c-siRNA