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C20orf186 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB4
Gene Name:  BPI fold containing family B, member 4
Sequence             
Original Clone ID: FLH265845.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00081221

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1725nts         Open reading frame : 1 to 1725
Reference Sequence Alignment

ATGCTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGT
GATATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAA
AAACTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTG
GGGGGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGA
AACAGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATAC
AGGGCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCT
GGAGTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCA
GCTGATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGAT
GGAGGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGC
ACTGTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTG
CGGCTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGG
AAGAGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGG
CTGACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTA
GGGGGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTG
AACCGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTG
GGTCTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGA
AGTGTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGAC
CTCAACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCA
GCTGTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAG
CTGGCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCT
CTAGACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACA
CTGGGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATC
AGGATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAG
GTGTTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGC
CTCATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATG
ATTGATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTT
GATATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCA
ATGAACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGC
AATGCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1728
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB4
Symbol Nomenclature : BPIFB4
SYNONYM : C20orf186; LPLUNC4; RY2G5; dJ726C3.5
Designation : BPI fold-containing family B member 4|ligand-binding protein RY2G5|likely ortholog of rat probable ligand-binding protein RY2G5|long palate, lung and nasal epithelium carcinoma associated 4|long palate, lung and nasal epithelium carcinoma-associated protein 4
Full Nomenclature : BPI fold containing family B, member 4
GENEID : 149954
GI : 147897710
GenBank Accession : BC140293
HGNC : 16179
Vega : OTTHUMG00000032235
Target GenBank: NM_182519

 

Reference Sequence Alignment

  

HsCD00081221      ------------------------------------------------------------
NM_182519.2       ATGTGGATGGCCTGGTGTGTGGCTGCGCTGTCTGTGGTGGCTGTGTGTGGCACCAGCCAC
                                                                              

HsCD00081221      ---------------------------------------------------------ATG
NM_182519.2       GAGACAAACACGGTCCTCAGGGTGACGAAAGATGTGTTGAGCAATGCCATTTCAGGCATG
                                                                           ***

HsCD00081221      CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
NM_182519.2       CTGCAGCAAAGTGATGCTCTCCACTCGGCCCTGAGAGAGGTGCCCTTGGGTGTTGGTGAT
                  ************************************************************

HsCD00081221      ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
NM_182519.2       ATTCCCTACAATGACTTCCATGTCCGAGGACCCCCCCCAGTATATACCAACGGCAAAAAA
                  ************************************************************

HsCD00081221      CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
NM_182519.2       CTTGATGGTATTTACCAGTATGGTCACATTGAGACCAACGACAACACTGCTCAGCTGGGG
                  ************************************************************

HsCD00081221      GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
NM_182519.2       GGCAAATACCGATATGGTGAGATCCTTGAGTCCGAGGGAAGCATCAGGGACCTCCGAAAC
                  ************************************************************

HsCD00081221      AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
NM_182519.2       AGTGGCTATCGCAGTGCCGAGAATGCATATGGAGGCCACAGGGGCCTCGGGCGATACAGG
                  ************************************************************

HsCD00081221      GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
NM_182519.2       GCAGCACCTGTGGGCAGGCTTCACCGGCGAGAGCTGCAGCCTGGAGAAATCCCACCTGGA
                  ************************************************************

HsCD00081221      GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
NM_182519.2       GTTGCCACTGGGGCGGTGGGCCCAGGTGGTTTGCTGGGCACTGGAGGCATGCTGGCAGCT
                  ************************************************************

HsCD00081221      GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
NM_182519.2       GATGGCATCCTCGCAGGCCAAGGTGGCCTGCTCGGCGGAGGTGGTCTCCTTGGTGATGGA
                  ************************************************************

HsCD00081221      GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
NM_182519.2       GGACTTCTTGGAGGAGGGGGTGTCCTGGGCGTGCTCGGCGAGGGTGGCATCCTCAGCACT
                  ************************************************************

HsCD00081221      GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
NM_182519.2       GTGCAAGGCATCACGGGGCTGCGTATCGTGGAGCTGACCCTCCCTCGGGTGTCCGTGCGG
                  ************************************************************

HsCD00081221      CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
NM_182519.2       CTCCTGCCCGGCGTGGGTGTCTACCTGAGCTTGTACACCCGTGTGGCCATCAACGGGAAG
                  ************************************************************

HsCD00081221      AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
NM_182519.2       AGTCTTATTGGCTTCCTGGACATCGCAGTAGAAGTGAACATCACAGCCAAGGTCCGGCTG
                  ************************************************************

HsCD00081221      ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
NM_182519.2       ACCATGGACCGCACGGGTTATCCTCGGCTGGTCATTGAGCGATGTGACACCCTCCTAGGG
                  ************************************************************

HsCD00081221      GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
NM_182519.2       GGCATCAAAGTCAAGCTGCTGCGAGGGCTTCTCCCCAATCTCGTGGACAATTTAGTGAAC
                  ************************************************************

HsCD00081221      CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
NM_182519.2       CGAGTCCTGGCCGACGTCCTCCCTGACTTGCTCTGCCCCATCGTGGATGTGGTGCTGGGT
                  ************************************************************

HsCD00081221      CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
NM_182519.2       CTTGTCAATGACCAGCTGGGCCTCGTGGATTCTCTGATTCCTCTGGGGATATTGGGAAGT
                  ************************************************************

HsCD00081221      GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
NM_182519.2       GTCCAGTACACCTTCTCCAGCCTCCCGCTTGTGACCGGGGAATTCCTGGAGCTGGACCTC
                  ************************************************************

HsCD00081221      AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
NM_182519.2       AACACGCTGGTTGGGGAGGCTGGAGGAGGACTCATCGACTACCCATTGGGGTGGCCAGCT
                  ************************************************************

HsCD00081221      GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
NM_182519.2       GTGTCTCCCAAGCCGATGCCAGAGCTGCCTCCCATGGGTGACAACACCAAGTCCCAGCTG
                  ************************************************************

HsCD00081221      GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
NM_182519.2       GCCATGTCTGCCAACTTCCTGGGCTCAGTGCTGACTCTACTGCAGAAGCAGCATGCTCTA
                  ************************************************************

HsCD00081221      GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
NM_182519.2       GACCTGGATATCACCAATGGCATGTTTGAAGAGCTTCCTCCACTTACCACAGCCACACTG
                  ************************************************************

HsCD00081221      GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
NM_182519.2       GGAGCCCTGATCCCCAAGGTGTTCCAGCAGTACCCCGAGTCCTGCCCACTTATCATCAGG
                  ************************************************************

HsCD00081221      ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
NM_182519.2       ATCCAGGTGCTGAACCCACCATCTGTGATGCTGCAGAAGGACAAAGCGCTGGTGAAGGTG
                  ************************************************************

HsCD00081221      TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
NM_182519.2       TTGGCCACTGCCGAGGTCATGGTCTCCCAGCCCAAAGACCTGGAGACTACCATCTGCCTC
                  ************************************************************

HsCD00081221      ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
NM_182519.2       ATTGACGTGGACACAGAATTCTTGGCCTCATTTTCCACAGAAGGAGATAAGCTCATGATT
                  ************************************************************

HsCD00081221      GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
NM_182519.2       GATGCCAAGCTGGAGAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGCAACTTTGAT
                  ************************************************************

HsCD00081221      ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
NM_182519.2       ATTGGCCTCATGGAGGTGCTGGTGGAGAAGATTTTTGACCTGGCATTCATGCCCGCAATG
                  ************************************************************

HsCD00081221      AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
NM_182519.2       AACGCTGTGCTGGGTTCTGGCGTCCCTCTCCCCAAAATCCTCAACATCGACTTTAGCAAT
                  ************************************************************

HsCD00081221      GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCA---
NM_182519.2       GCAGACATTGACGTGTTGGAGGACCTTTTGGTGCTGAGCGCATGA
                  ******************************************   

Protein

SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
UniProt : P59827
HPRD : 16645

Clone

Cloning Information : 265845
HIP Master Clone ID : 197943
IMAGE ID : 100014255
Original Clone ID : FLH265845.01L

Organism


TAX_ID : 9606
Species Specific ID: 149954

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186191

Reagents

Labome : BPIFB4-antibody