Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Cloned using restriction enzymes, XbaI and NotI, in fusion with mCherry
Species:
Human alphaherpesvirus 1
Type:
cDNA
Vector Name:
MV14
Format:
FUSION
Source:
Karmella Haynes
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Arizona State University
Rene Daer
Karmella Haynes
Cassandra Barrett
Rene Daer
Karmella Haynes
Cassandra Barrett
HaCD00812388
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 150nts
Open reading frame: 1 to 150
GACGCTTTGGACGACTTCGACTTGGACATGTTGGGTTCTGACGCTTTGGACGACTTCGAC
TTGGACATGTTGGGTTCTGACGCTTTGGACGACTTCGACTTGGACATGTTGGGTTCTGAC
GCTTTGGACGACTTCGACTTGGACATGTTG
Protein sequence predicted based on nucleotide sequence
DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDML
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verified by Clone submitter
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: puromycin
Host Type: mammalian Marker: puromycin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
MV14
Synonyms:
''
Description:
fluorescent marker assay, multiple cloning site clone, mammalian expression expression with AmpR and CMV and Gal4 Act and mCherry and F1 origin and NotI and XbaI and SV40 and PuroR; puromycin resistance in mammalian, ampicillin resistance in bacterial;
Comments:
This vector is pRL-CMV modified by addition of a ccdB death cassette to make it compatible with the Gateway system.
Size (bp):
6362
Parent Vector:
None
Properties:
fluorescent marker, mammalian expression, multiple cloning site
Author Name:
Karmella Haynes
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
Gene
mCherry
2127
2831
Miscellaneous Feature
NotI
NotI Cut Site
2866
2873
Miscellaneous Feature
XbaI
XbaI Cut Site
2832
2837
Origin
F1 origin
3251
3557
binding site
Gal4 Act
Gal4 DNA Binding Domain
1662
2102
enhancer
SV40
SV40 Enhancer
3677
3892
promoter
CMV
1025
1624
selectable marker
AmpR
9
653
selectable marker
PuroR
4015
4614
