Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Vector map has been attached to file. Insert begins with Tet promoter followed by a Kozac sequence. Coding region begins with the part mCherry (a red fluorescent protein acting as a reporter gene), and then followed by VP64, a tetrameric r
Species:
Recombinant
Type:
cDNA
Vector Name:
MV2
Format:
CLOSED
Source:
Karmella Haynes
Description:
Vector map has been attached to file. Insert begins with Tet promoter followed by a Kozac sequence. Coding region begins with the part mCherry (a red fluorescent protein acting as a reporter gene), and then followed by VP64, a tetrameric repeat of the herpes simplex virus (HSV)-1 VP16 activation domain. Information can be found here: https://benchling.com/s/seq-HVFDP0Ic3Q4xS5IgFsgz
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
Authors:
Arizona State University
Karmella Haynes
Pamela Silver
Karmella Haynes
Pamela Silver
ReCD00812742
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 936nts
Open reading frame: 746 to 1681
GTTGACATTGATTATTGGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATA
GCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGC
CCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAG
GGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTAC
ATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCG
CCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACG
TATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGAT
AGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGT
TTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGC
AAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCCCTATCAGTG
ATAGAGATCTCCCTATCAGTGATAGAGATCGTCGACGAGCTCGTTTAGTGAACCGTCAGA
TCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCA
GCCTCCGACTAGACCCGCCGCCACCATGGAGACTAGAGTGAGCAAGGGCGAGGAGGATAA
CATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGG
CCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGC
CAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCA
GTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAA
GCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGT
GGTGACCGTGACCCAGGACTCCTCCTTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCT
GCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGA
GGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAG
GCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAA
GAAGCCCGTGCAGCTACCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCA
CAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGG
CGGCATGGACGAGCTGTACAAGACTAGAGACGCTTTGGACGACTTCGACTTGGACATGTT
GGGTTCTGACGCTTTGGACGACTTCGACTTGGACATGTTGGGTTCTGACGCTTTGGACGA
CTTCGACTTGGACATGTTGGGTTCTGACGCTTTGGACGACTTCGACTTGGACATGTTGAC
TAGACCCAAGAAAAAGCGCAAGGTAGAACAAAAACTCATCTCAGAAGAGGATCTGGCGTA
AAGCTGAG
Protein sequence predicted based on nucleotide sequence
METRVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGG PLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSS LQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGH YDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKT RDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLTRPKKKRKV EQKLISEEDLA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verified by Clone submitter
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: puromycin
Host Type: mammalian Marker: puromycin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
MV2
Synonyms:
''
Description:
fluorescent marker assay, multiple cloning site clone, mammalian expression expression with AmpR and DD123B and DD122B and F1 origin and SV40 and PuroR and lac and SpeI; ampicillin resistance in bacterial, puromycin resistance in mammalian;
Comments:
This vector is pRL-CMV modified by addition of a ccdB death cassette to make it compatible with the Gateway system.
Size (bp):
4521
Parent Vector:
None
Properties:
fluorescent marker, mammalian expression, multiple cloning site
Author Name:
Karmella Haynes
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
Miscellaneous Feature
SpeI
SpeI Cut Site
18
23
Origin
F1 origin
390
696
enhancer
SV40
816
1031
primer
DD122B
5' Sequencing Primer - gttaggcgttttgcgctg
4476
4493
primer
DD123B
Sequencing Primer - gctggcaactagaaggcac
105
123
promoter
lac
2185
2214
selectable marker
PuroR
Puromycin Resistance
1154
1753
selectable marker
AmpR
3294
4154
