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Detailed Vector Information: pRSF-BAD

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pRSF-BAD              
Source: None
Description: ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
Comments: None
Publications: None
Authors: Dr. Debbie Hansen
EvNO00813765

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AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  50ug/mL of Kanamycin
       DyNA Vector Map

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Vector Name: pRSF-BAD

pRSF-BAD in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  ARAC5R
Reverse:  T7 terminator
Description: ligation independent cloning (LIC) clone, bacterial expression expression with Kan-R and T7TER primer (#35) and T7 Terminator and RNA II and RNA I and RSF ori - RSF1030 Homology and -10 RNA I and -35 RNA I and -35 AP and -10 AP and RBS-reverse-weak and araC and Operator O2 and araC promoter and Operator O1 and CAP site and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ligation junction Xbal and BADT1A Primer and BADT1B Primer and ARAC3F primer (#145) and ARAC5R primer (#148) and wrong primer #62 off by 3 nt and KAN1F primer (#120) and ligation junction Pacl; 50ug/mL of Kanamycin resistance in bacterial;
Comments: TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp): 3600
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC)
Author Name: Dr. Debbie Hansen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
-10 signal -10 RNA I 3420 3425
-10 signal -10 AP 2777 2783
-35 signal -35 AP 2800 2805
-35 signal -35 RNA I 3396 3401
CDS araC 109 987
CDS Kan-R 1920 2735
Miscellaneous Feature ligation junction Xbal 3595 3600
Miscellaneous Feature ligation junction Pacl 1606 1613
Miscellaneous Feature Operator I1 and I2 1226 1264
Miscellaneous Feature CAP site 1217 1230
Miscellaneous Feature Operator O1 1174 1195
Miscellaneous Feature RBS 1318 1327
Miscellaneous Feature Operator O2 1016 1033
Miscellaneous RNA RNA II 2843 3541
Miscellaneous RNA RNA I 3432 3538
RBS RBS-reverse-weak 2741 2749
Terminator rrnB T1 T2 1356 1587
Terminator T7 Terminator 1643 1690
primer T7TER primer (#35) 1629 1647
primer KAN1F primer (#120) 1929 1950
primer wrong primer #62 off by 3 nt 1275 1296
primer BADT1A Primer 373 392
primer BADT1B Primer 830 849
primer ARAC3F primer (#145) 118 138
primer ARAC5R primer (#148) 964 983
promoter araC promoter 1138 1166
promoter arabinose BAD promoter 1263 1290
rep_origin RSF ori - RSF1030 Homology 2843 3592

 

Species Specific ID: None
Target GenBank: None