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Detailed Vector Information: pCPD-pRL-CMV-ccdb

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pCPD-pRL-CMV-ccdb              
Source: CPD
Description: fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments: None
Publications: None
Authors: Shane Miersch
EvNO00813803

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AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  kanamycin
Growth Condition:  Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:  None
       DyNA Vector Map

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Vector Name: pCPD-pRL-CMV-ccdb

pCPD-pRL-CMV-ccdb in advanced viewed

Synonyms: Modified pRL-CMV
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments: This vector is pRL-CMV modified by addition of a ccdB death cassette to make it compatible with the Gateway system.
Size (bp): 5870
Parent Vector: Name: pRL-CMV
Description: Reporter vector with Renilla luciferase from Promega with CMV and T7 promoters; ampicillin resistance in bacteria.
Properties: Gateway, bacterial expression, fluorescent marker
Author Name: Shane Miersch
Publications: PMID: 25758251
Title: Antiviral antibody profiling by high-density protein arrays.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
fluorescent marker Renilla Luciferase Renilla luciferase gene 1912 2844
negative selection marker ccdB Ccd death gene present in 'empty vector' form only (replace with gene of interest) 1432 1737
primer pCEP Fwd Primer pCEP_Fwd_Primer 722 741
primer CMV-F CMV_fwd_Primer 678 698
promoter CAGF CAG enhancer 224 511
promoter Amp-R Ampicillin resistance 4184 5044
promoter lac promoter Lac promoter 346 369
promoter T7 promoter T7 Promoter 90 112
promoter CMV CMV_immearly_promoter 169 721
recombination site attR1 AttR1 recombination site, used in Gateway cloning 197 321
recombination site attR2 AttR2 recombination site, Gateway cloning 1778 1879
selectable marker Chloramphenicol-R Chloramphenical resistance gene 430 1089

 

Species Specific ID: None
Target GenBank: None