fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Growth Condition:
Growth in 50 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Vector Name:
pCPD-pRL-CMV-ccdb
Synonyms:
Modified pRL-CMV
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
fluorescent marker assay, Gateway cloning, bacterial expression expression with Renilla Luciferase and pCEP Fwd Primer and ccdB and CMV and CAGF and CMV-F and Amp-R and lac promoter and attR1 and attR2 and T7 promoter and Chloramphenicol-R; chloramphenicol resistance in bacterial, ampicillin resistance in bacterial;
Comments:
This vector is pRL-CMV modified by addition of a ccdB death cassette to make it compatible with the Gateway system.