PMFBP1 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PMFBP1
Gene Name:  polyamine modulated factor 1 binding protein 1
Original Clone ID:
FLH266024.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081400
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3021nts
Open reading frame: 1 to 3021

ATGAAAGATGAGGCGGGGGAGAGAGACAGAGAAGTGAGCAGCCTGAACAGCAAGCTGTTA
AGCCTGCAACTTGACATCAAGAATCTGCACGATGTCTGCAAGAGACAGAGGAAGACCTTG
CAGGACAATCAGCTCTGCATGGAGGAGGCAATGAACAGCAGCCACGACAAGAAGCAAGCA
CAGGCATTAGCATTCGAGGAGTCAGAGGTGGAATTTGGGTCCAGTAAACAGTGTCATCTG
AGACAACTCCAGCAACTGAAGAAAAAATTGCTGGTCCTTCAACAAGAACTGGAGTTTCAC
ACAGAGGAGTTGCAGACTTCTTACTATTCTCTCCGCCAGTATCAGTCCATCCTAGAGAAG
CAGACTTCCGACCTGGTTCTTCTGCACCATCACTGCAAACTGAAAGAAGATGAGGTGATT
CTCTATGAGGAGGAAATGGGAAATCACAACGAGAACACAGGGGAGAAGCTCCATTTGGCG
CAGGAGCAACTCGCCTTGGCCGGGGACAAGATCGCCTCTCTAGAGAGGAGCTTAAACCTC
TACAGGGATAAATACCAGTCTTCCCTGAGCAACATCGAGTTACTAGAATGCCAAGTGAAG
ATGTTGCAGGGGGAACTCGGCGGGATCATGGGTCAGGAGCCTGAGAACAAGGGTGATCAT
TCAAAGGTACGGATATACACTTCTCCTTGCATGATTCAAGAGCATCAGGAGACTCAGAAA
CGACTGTCTGAAGTCTGGCAAAAGGTCTCTCAACAGGATGATCTCATTCAAGAACTTCGA
AATAAGCTGGCCTGCAGTAACGCTTTGGTTCTGGAGCGTGAAAAGGCTTTGATAAAACTA
CAAGCCGATTTTGCTTCCTGTACAGCCACCCACAGATACCCTCCTAGCTCCTCAGAAGAG
TGTGAAGACATCAAAAAGATACTGAAGCACTTGCAGGAGCAGAAAGACAGCCAGTGCCTG
CATGTGGAGGAGTACCAGAACCTGGTGAAGGATCTGCGCGTGGAACTAGAGGCCGTGTCG
GAACAGAAGAGAAACATCATGAAGGACATGATGAAGCTGGAGCTGGACCTGCACGGACTG
CGGGAGGAGACATCTGCCCACATTGAGAGGAAGGATAAGGACATCACCATCCTGCAGTGC
CGGCTGCAGGAGCTGCAGCTGGAGTTCACCGAGACCCAAAAGCTCACTTTGAAGAAAGAC
AAGTTCCTCCAAGAGAAAGATGAGATGCTGCAAGAGCTGGAGAAGAAACTGACACAGGTT
CAGAACAGCCTCCTGAAAAAGGAGAAGGAGCTGGAGAAGCAGCAGTGCATGGCCACAGAA
CTTGAAATGACAGTCAAGGAGGCTAAGCAGGACAAGTCCAAGGAGGCGGAGTGCAAGGCC
CTGCAGGCTGAGGTCCAGAAGCTGAAGAACAGTCTCGAAGAGGCCAAGCAGCAGGAGAGG
CTGGCTGCTCAGCAAGCAGCCCAGTGCAAAGAAGAGGCTGCACTGGCAGGCTGTCACCTG
GAGGACACCCAGAGGAAACTGCAGAAGGGTCTCCTCCTGGACAAGCAGAAGGCAGACACC
ATCCAGGAACTACAGAGAGAACTTCAGATGCTGCAGAAGGAGTCCTCGATGGCTGAGAAG
GAACAAACCTCCAACAGAAAACGGGTGGAGGAGCTGTCATTAGAACTCTCTGAAGCCCTG
AGGAAGCTTGAAAATTCAGACAAGGAAAAGAGGCAGCTTCAGAAGACAGTGGCTGAGCAG
GATATGAAAATGAATGACATGCTTGATCGTATCAAGCACCAGCACAGGGAGCAAGGCTCC
ATCAAATGCAAGTTAGAAGAAGATCTTCAGGAGGCCACAAAGCTTCTGGAGGACAAACGG
GAGCAGTTGAAGAAGAGCAAAGAGCATGAGAAGCTGATGGAGGGAGAACTTGAAGCTTTG
CGGCAGGAATTTAAAAAGAAAGACAAGACGTTGAAAGAGAATTCCAGAAAGTTGGAGGAA
GAAAATGAGAATCTCCGAGCAGAGCTACAGTGTTGTTCTACACAACTGGAATCCTCTCTC
AACAAATACAACACCAGCCAGCAAGTCATCCAAGACTTGAATAAAGAGATAGCCCTTCAG
AAGGAGTCCTTAATGAGCCTGCAGGCCCAGCTGGACAAAGCTCTGCAGAAGGAGAAGCAC
TATCTCCAGACTACCATCACCAAAGAAGCCTATGATGCATTATCCCGGAAGTCAGCCGCC
TGCCAGGATGACCTGACACAAGCCCTCGAGAAGCTCAATCACGTGACCTCAGAGACAAAG
AGCCTGCAGCAAAGCTTGACACAGACCCAAGAGAAGAAAGCTCAGCTGGAAGAGGAAATC
ATTGCTTATGAGGAAAGGATGAAAAAGCTCAATACGGAATTAAGAAAACTGCGGGGCTTC
CACCAGGAGAGTGAGCTGGAGGTGCACGCCTTTGACAAGAAGCTAGAGGAGATGAGCTGC
CAGGTGCTGCAGTGGCAGAAGCAACACCAGAATGACCTCAAGATGCTGGCAGCCAAAGAG
GAGCAGCTCAGGGAGTTCCAGGAGGAGATGGCCGCCTTAAAAGAGAACCTCCTTGAGGAC
GATAAGGAGCCCTGCTGCCTGCCCCAGTGGTCTGTGCCCAAAGACACCTGTAGGCTCTAC
CGAGGGAATGATCAGATTATGACCAACTTGGAGCAATGGGCAAAACAGCAGAAGGTCGCC
AATGAGAAACTAGGAAACCAGCTCCGAGAGCAGGTGAACTACATTGCCAAGCTGAGTGGC
GAAAAGGACCACCTCCACAGTGTAATGGTCCACTTGCAGCAGGAAAACAAGAAGCTGAAG
AAGGAGATAGAAGAGAAGAAGATGAAAGCCGAGAACACAAGGCTATGCACCAAAGCCCTA
GGCCCGAGCAGAACGGAGTCCACACAGAGGGAGAAAGTGTGCGGCACCTTGGGCTGGAAG
GGGTTGCCCCAGGATATGGGTCAAAGAATGGACCTCACCAAGTACATCGGGATGCCCCAC
TGCCCGGGTTCCTCATACTGC

Protein sequence predicted based on nucleotide sequence

MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQA
QALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEK
QTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNL
YRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQK
RLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSSSEE
CEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELDLHGL
REETSAHIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV
QNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQER
LAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEK
EQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGS
IKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEE
ENENLRAELQCCSTQLESSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKH
YLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEI
IAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKE
EQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVA
NEKLGNQLREQVNYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKAL
GPSRTESTQREKVCGTLGWKGLPQDMGQRMDLTKYIGMPHCPGSSYC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 133
End on reference sequence 3156
Mutations:
None
Discrepancy:
a2414g,E805G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable