Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
U0687BI160-113
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00814563
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5049nts
Open reading frame: 1 to 5049
ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCGCTGCACGGGAAGCCTTTGCCCTTGGG
GGCCTGAGCTGTGCTGGGGCCTGGAGCTCCTGCCCGCCTCATCCCCCTCCTCGTAGCGCA
TGGCTGCCTGGAGGCAGATGCTCAGCCAGCATTGGGCAGCCCCCGCTTCCTGCTCCCCTA
CCCCCTTCACATGGCAGTAGTTCTGGGCACCCCAGCAAACCATATTATGCTCCAGGGGCG
CCCACTCCAAGACCCCTCCATGGGAAGCTGGAATCCCTGCATGGCTGTGTGCAGGCATTG
CTCCGGGAGCCAGCCCAGCCAGGGCTTTGGGAACAGCTTGGGCAACTGTACGAGTCAGAG
CACGATAGTGAGGAGGCCACACGCTGCTACCACAGCGCCCTTCGATACGGAGGAAGCTTC
GCTGAGCTGGGGCCCCGCATTGGCCGACTGCAGCAGGCCCAGCTCTGGAACTTTCATACT
GGCTCCTGCCAGCACCGAGCCAAGGTCCTGCCCCCACTGGAGCAAGTGTGGAACTTGCTA
CACCTTGAGCACAAACGGAACTATGGAGCCAAGCGGGGAGGTCCCCCGGTGAAGCGAGCT
GCTGAACCCCCAGTGGTGCAGCCTGTGCCTCCTGCAGCACTCTCAGGCCCCTCAGGGGAG
GAGGGCCTCAGCCCTGGAGGCAAGCGAAGGAGAGGCTGCAACTCTGAACAGACTGGCCTT
CCCCCAGGGCTGCCACTGCCTCCACCACCATTACCACCACCACCACCACCACCACCACCA
CCACCACCACCCCTGCCTGGCCTGGCTACCAGCCCCCCATTTCAGCTAACCAAGCCAGGG
CTGTGGAGTACCCTGCATGGAGATGCCTGGGGCCCAGAGCGCAAGGGTTCAGCACCCCCA
GAGCGCCAGGAGCAGCGGCACTCGCTGCCTCACCCATATCCATACCCAGCTCCAGCGTAC
ACCGCGCACCCCCCTGGCCACCGGCTGGTCCCGGCTGCTCCCCCAGGCCCAGGCCCCCGC
CCCCCAGGAGCAGAGAGCCATGGCTGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTT
AGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACTCCAGCGTTTCACCAGCAGCAACCACC
GCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGGCACCACCACCAGCAGC
AGCAGTAGCAGCAGCAGCAACACTGGTCTCCGGGGCGTGGAGCCGAACCCAGGCATTCCC
GGCGCTGACCATTACCAAACTCCCGCGCTGGAGGTCTCTCACCATGGCCGCCTGGGGCCC
TCGGCACACAGCAGTCGGAAACCGTTCTTGGGGGCTCCCGCTGCCACTCCCCACCTATCC
CTGCCACCTGGACCTTCCTCACCCCCTCCACCCCCCTGTCCCCGCCTCTTACGCCCCCCA
CCACCCCCTGCCTGGTTGAAGGGTCCGGCCTGCCGGGCAGCCCGAGAGGATGGAGAGATC
TTAGAAGAGCTCTTCTTTGGGACTGAGGGACCCCCCCGCCCTGCCCCACCACCCCTCCCC
CATCGCGAGGGCTTCTTGGGGCCTCCGGCCTCCCGCTTTTCTGTGGGCACTCAGGATTCT
CACACCCCTCCCACTCCCCCAACCCCAACCACCAGCAGTAGCAACAGCAACAGTGGCAGC
CACAGCAGCAGCCCTGCTGGGCCTGTGTCCTTTCCCCCACCACCCTATCTGGCCAGAAGT
ATAGACCCCCTTCCCCGGCCTCCCAGCCCAGCACAGAACCCCCAGGACCCACCTCTTGTA
CCCCTGACTCTTGCCCTGCCTCCAGCCCCTCCTTCCTCCTGCCACCAAAATACCTCAGGA
AGCTTCAGGCGCCCGGAGAGCCCCCGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTG
GGGCCGGGGCCACCCCCAGGCCCCCTGAGTAAAGCCCCCCAGCCTGTGCCGCCCGGGGTT
GGGGAGCTGCCTGCCCGAGGCCCTCGACTCTTTGATTTTCCCCCCACTCCGCTGGAGGAC
CAGTTTGAGGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCCAACATCATGAAG
ATGCTGGACGAATCCATTCGCAAGGAAGAGGAACAGCAACAACACGAAGCAGGCGTGGCC
CCCCAACCCCCGCTGAAGGAGCCCTTTGCATCTCTGCAGTCTCCTTTCCCCACCGACACA
GCCCCCACCACTACTGCTCCTGCTGTCGCCGTCACCACCACCACCACCACCACCACCACC
ACCACGGCCACCCAGGAAGAGGAGAAGAAGCCACCACCAGCCCTACCACCACCACCGCCT
CTAGCCAAGTTCCCTCCACCCTCTCAGCCACAGCCACCACCACCCCCACCCCCCAGCCCG
GCCAGCCTGCTCAAATCCTTGGCCTCCGTGCTGGAGGGACAAAAGTACTGTTATCGGGGG
ACTGGAGCAGCTGTTTCCACCCGGCCTGGGCCCTTGCCCACCACTCAGTATTCCCCTGGC
CCCCCATCAGGTGCTACCGCCCTGCCGCCCACCTCAGCGGCCCCTAGCGCCCAGGGCTCC
CCACAGCCCTCTGCTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCCTGGGCCCGG
GAGCGCAGGGCGGGCGAAGAGCCAGTCCCGGGCCCCATGACCCCCACCCAACCGCCCCCA
CCCCTATCTCTGCCCCCTGCTCGCTCTGAGTCTGAGGTGCTAGAAGAGATCAGCCGGGCT
TGCGAGACCCTTGTGGAGCGGGTGGGCCGGAGTGCCACTGACCCAGCCGACCCAGTGGAC
ACAGCAGAGCCAGCGGACAGTGGGACTGAGCGACTGCTGCCCCCCGCACAGGCCAAGGAG
GAGGCTGGCGGGGTGGCGGCAGTGTCAGGCAGCTGTAAGCGGCGACAGAAGGAGCATCAG
AAGGAGCATCGGCGGCACAGGCGGGCCTGTAAGGACAGTGTGGGTCGTCGGCCCCGTGAG
GGCAGGGCAAAGGCCAAGGCCAAGGTCCCCAAAGAAAAGAGCCGCCGGGTGCTGGGGAAC
CTGGACCTGCAGAGCGAGGAGATCCAGGGTCGTGAGAAGTCCCGGCCCGATCTTGGCGGG
GCCTCCAAGGCCAAGCCACCCACAGCTCCAGCCCCTCCATCAGCTCCTGCACCTTCTGCC
CAGCCCACACCCCCGTCAGCCTCTGTCCCTGGAAAGAAGGCTCGGGAGGAAGCCCCAGGG
CCACCGGGTGTCAGCCGGGCCGACATGCTGAAGCTGCGCTCACTTAGTGAGGGGCCCCCC
AAGGAGCTGAAGATCCGGCTCATCAAGGTAGAGAGTGGTGACAAGGAGACCTTTATCGCC
TCTGAGGTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCTGCT
GACGTCGTGCGCGCCAGCAGGAATGCCAAGGTGAAAGGGAAGTTTCGAGAGTCCTACCTT
TCCCCTGCCCAGTCTGTGAAACCGAAGATCAACACTGAGGAGAAGCTGCCCCGGGAAAAA
CTCAACCCCCCTACACCCAGCATCTATCTGGAGAGCAAACGGGATGCCTTCTCACCTGTC
CTGCTGCAGTTCTGTACAGACCCTCGAAATCCCATCACAGTGATCCGGGGCCTGGCGGGC
TCCCTGCGGCTCAACTTGGGCCTCTTCTCCACCAAGACCCTGGTGGAAGCGAGTGGCGAA
CACACCGTGGAAGTTCGCACCCAGGTGCAGCAGCCCTCAGATGAGAACTGGGATCTGACA
GGCACTCGGCAGATCTGGCCTTGTGAGAGCTCCCGTTCCCACACCACCATTGCCAAGTAC
GCACAGTACCAGGCCTCATCCTTCCAGGAGTCTCTGCAGGAGGAGAAGGAGAGTGAGGAT
GAGGAGTCAGAGGAGCCAGACAGCACCACTGGAACCCCTCCTAGCAGCGCACCAGACCCG
AAGAACCATCACATCATCAAGTTTGGCACCAACATCGACTTGTCTGATGCTAAGCGGTGG
AAGCCCCAGCTGCAGGAGCTGCTGAAGCTGCCCGCCTTCATGCGGGTAACATCCACGGGC
AACATGCTGAGCCACGTGGGCCACACCATCCTGGGCATGAACACGGTGCAGCTGTACATG
AAGGTGCCCGGCAGCCGAACGCCAGGCCACCAGGAGAATAACAACTTCTGCTCCGTCAAC
ATCAACATTGGCCCAGGCGACTGCGAGTGGTTCGCGGTGCACGAGCACTACTGGGAGACC
ATCAGCGCTTTCTGTGATCGGCACGGCGTGGACTACTTGACGGGTTCCTGGTGGCCAATC
CTGGATGATCTCTATGCATCCAATATTCCTGTGTACCGCTTCGTGCAGCGACCCGGAGAC
CTCGTGTGGATTAATGCGGGGACTGTGCACTGGGTGCAGGCCACCGGCTGGTGCAACAAC
ATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGGCCCTGGAACGATAC
GAGTGGAATGAGGTGAAGAACGTCAAATCCATCGTGCCCATGATTCACGTGTCATGGAAC
GTGGCTCGCACGGTCAAAATCAGCGACCCCGACTTGTTCAAGATGATCAAGTTCTGCCTG
CTGCAGTCCATGAAGCACTGCCAGGTGCAACGCGAGAGCCTGGTGCGGGCAGGGAAGAAA
ATCGCTTACCAGGGCCGTGTCAAGGACGAGCCAGCCTACTACTGCAACGAGTGCGATGTG
GAGGTGTTTAACATCCTGTTCGTGACAAGTGAGAATGGCAGCCGCAACACGTACCTGGTA
CACTGCGAGGGCTGTGCCCGGCGCCGCAGCGCAGGCCTGCAGGGCGTGGTGGTGCTGGAG
CAGTACCGCACTGAGGAGCTGGCTCAGGCCTACGACGCCTTCACGCTGGTGAGGGCCCGG
CGGGCGCGCGGGCAGCGGAGGAGGGCACTGGGGCAGGCTGCAGGGACGGGCTTCGGGAGC
CCGGCCGCGCCTTTCCCTGAGCCCCCGCCGGCTTTCTCCCCCCAGGCCCCAGCCAGCACG
TCGCGATTG
Protein sequence predicted based on nucleotide sequence
MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLPAPL PPSHGSSSGHPSKPYYAPGAPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESE HDSEEATRCYHSALRYGGSFAELGPRIGRLQQAQLWNFHTGSCQHRAKVLPPLEQVWNLL HLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCNSEQTGL PPGLPLPPPPLPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGSAPP ERQEQRHSLPHPYPYPAPAYTAHPPGHRLVPAAPPGPGPRPPGAESHGCLPATRPPGSDL RESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSNTGLRGVEPNPGIP GADHYQTPALEVSHHGRLGPSAHSSRKPFLGAPAATPHLSLPPGPSSPPPPPCPRLLRPP PPPAWLKGPACRAAREDGEILEELFFGTEGPPRPAPPPLPHREGFLGPPASRFSVGTQDS HTPPTPPTPTTSSSNSNSGSHSSSPAGPVSFPPPPYLARSIDPLPRPPSPAQNPQDPPLV PLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPPPGPLSKAPQPVPPGV GELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQHEAGVA PQPPLKEPFASLQSPFPTDTAPTTTAPAVAVTTTTTTTTTTTATQEEEKKPPPALPPPPP LAKFPPPSQPQPPPPPPPSPASLLKSLASVLEGQKYCYRGTGAAVSTRPGPLPTTQYSPG PPSGATALPPTSAAPSAQGSPQPSASSSSQFSTSGGPWARERRAGEEPVPGPMTPTQPPP PLSLPPARSESEVLEEISRACETLVERVGRSATDPADPVDTAEPADSGTERLLPPAQAKE EAGGVAAVSGSCKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKAKAKVPKEKSRRVLGN LDLQSEEIQGREKSRPDLGGASKAKPPTAPAPPSAPAPSAQPTPPSASVPGKKAREEAPG PPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAA DVVRASRNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPV LLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLT GTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDP KNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYM KVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPI LDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERY EWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKK IAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAGLQGVVVLE QYRTEELAQAYDAFTLVRARRARGQRRRALGQAAGTGFGSPAAPFPEPPPAFSPQAPAST SRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
