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BAG1 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BAG1
Gene Name:  BCL2-associated athanogene
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266216.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24949977
Title: Glucocorticoid Receptor Function Regulated by Coordinated Action of the Hsp90 and Hsp70 Chaperone Cycles
Authors: The ORFeome Collaboration
HsCD00081592

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 822nts         Open reading frame : 1 to 822
Reference Sequence Alignment

ATGAAGAAGAAAACCCGGCGCCGCTCGACCCGGAGCGAGGAGTTGACCCGGAGCGAGGAG
TTGACCCTGAGTGAGGAAGCGACCTGGAGTGAAGAGGCGACCCAGAGTGAGGAGGCGACC
CAGGGCGAAGAGATGAATCGGAGCCAGGAGGTGACCCGGGACGAGGAGTCGACCCGGAGC
GAGGAGGTGACCAGGGAGGAAATGGCGGCAGCTGGGCTCACCGTGACTGTCACCCACAGC
AATGAGAAGCACGACCTTCATGTTACCTCCCAGCAGGGCAGCAGTGAACCAGTTGTCCAA
GACCTGGCCCAGGTTGTTGAAGAGGTCATAGGGGTTCCACAGTCTTTTCAGAAACTCATA
TTTAAGGGAAAATCTCTGAAGGAAATGGAAACACCGTTGTCAGCACTTGGAATACAAGAT
GGTTGCCGGGTCATGTTAATTGGGAAAAAGAACAGTCCACAGGAAGAGGTTGAACTAAAG
AAGTTGAAACATTTGGAGAAGTCTGTGGAGAAGATAGCTGACCAGCTGGAAGAGTTGAAT
AAAGAGCTTACTGGAATCCAGCAGGGTTTTCTGCCCAAGGATTTGCAAGCTGAAGCTCTC
TGCAAACTTGATAGGAGAGTAAAAGCCACAATAGAGCAGTTTATGAAGATCTTGGAGGAG
ATTGACACACTGATCCTGCCAGAAAATTTCAAAGACAGTAGATTGAAAAGGAAAGGCTTG
GTAAAAAAGGTTCAGGCATTCCTAGCCGAGTGTGACACAGTGGAGCAGAACATCTGCCAG
GAGACTGAGCGGCTGCAGTCTACAAACTTTGCCCTGGCCGAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1086
Start on reference sequence 262
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BAG1
Symbol Nomenclature : BAG1
SYNONYM : BAG-1; HAP; RAP46
Designation : BAG family molecular chaperone regulator 1|Bcl-2 associating athanogene-1 protein|Bcl-2-binding protein|glucocortoid receptor-associated protein RAP46|receptor-associated protein, 46-KD
Full Nomenclature : BCL2-associated athanogene
GENEID : 573
Locus Tag : RP11-326F20.2
GI : 117646661
GenBank Accession : AM392568
HGNC : 937
MIM : 601497
Vega : OTTHUMG00000019766
Target GenBank: BC001936

 

Reference Sequence Alignment

  

HsCD00081592      ------------------------------------------------------------
NM_004323.5       CTGGCTCAGCGCGGGGGGGCGCGGAGACCGCGAGGCGACCGGGAGCGGCTGGGTTCCCGG
                                                                              

HsCD00081592      ------------------------------------------------------------
NM_004323.5       CTGCGCGCCCTTCGGCCAGGCCGGGAGCCGCGCCAGTCGGAGCCCCCGGCCCAGCGTGGT
                                                                              

HsCD00081592      ------------------------------------------------------------
NM_004323.5       CCGCCTCCCTCTCGGCGTCCACCTGCCCGGAGTACTGCCAGCGGGCATGACCGACCCACC
                                                                              

HsCD00081592      ---------------------------------ATGAAGAAGAAAACCCGGCGCCGCTCG
NM_004323.5       AGGGGCGCCGCCGCCGGCGCTCGCAGGCCGCGGATGAAGAAGAAAACCCGGCGCCGCTCG
                                                   ***************************

HsCD00081592      ACCCGGAGCGAGGAGTTGACCCGGAGCGAGGAGTTGACCCTGAGTGAGGAAGCGACCTGG
NM_004323.5       ACCCGGAGCGAGGAGTTGACCCGGAGCGAGGAGTTGACCCTGAGTGAGGAAGCGACCTGG
                  ************************************************************

HsCD00081592      AGTGAAGAGGCGACCCAGAGTGAGGAGGCGACCCAGGGCGAAGAGATGAATCGGAGCCAG
NM_004323.5       AGTGAAGAGGCGACCCAGAGTGAGGAGGCGACCCAGGGCGAAGAGATGAATCGGAGCCAG
                  ************************************************************

HsCD00081592      GAGGTGACCCGGGACGAGGAGTCGACCCGGAGCGAGGAGGTGACCAGGGAGGAAATGGCG
NM_004323.5       GAGGTGACCCGGGACGAGGAGTCGACCCGGAGCGAGGAGGTGACCAGGGAGGAAATGGCG
                  ************************************************************

HsCD00081592      GCAGCTGGGCTCACCGTGACTGTCACCCACAGCAATGAGAAGCACGACCTTCATGTTACC
NM_004323.5       GCAGCTGGGCTCACCGTGACTGTCACCCACAGCAATGAGAAGCACGACCTTCATGTTACC
                  ************************************************************

HsCD00081592      TCCCAGCAGGGCAGCAGTGAACCAGTTGTCCAAGACCTGGCCCAGGTTGTTGAAGAGGTC
NM_004323.5       TCCCAGCAGGGCAGCAGTGAACCAGTTGTCCAAGACCTGGCCCAGGTTGTTGAAGAGGTC
                  ************************************************************

HsCD00081592      ATAGGGGTTCCACAGTCTTTTCAGAAACTCATATTTAAGGGAAAATCTCTGAAGGAAATG
NM_004323.5       ATAGGGGTTCCACAGTCTTTTCAGAAACTCATATTTAAGGGAAAATCTCTGAAGGAAATG
                  ************************************************************

HsCD00081592      GAAACACCGTTGTCAGCACTTGGAATACAAGATGGTTGCCGGGTCATGTTAATTGGGAAA
NM_004323.5       GAAACACCGTTGTCAGCACTTGGAATACAAGATGGTTGCCGGGTCATGTTAATTGGGAAA
                  ************************************************************

HsCD00081592      AAGAACAGTCCACAGGAAGAGGTTGAACTAAAGAAGTTGAAACATTTGGAGAAGTCTGTG
NM_004323.5       AAGAACAGTCCACAGGAAGAGGTTGAACTAAAGAAGTTGAAACATTTGGAGAAGTCTGTG
                  ************************************************************

HsCD00081592      GAGAAGATAGCTGACCAGCTGGAAGAGTTGAATAAAGAGCTTACTGGAATCCAGCAGGGT
NM_004323.5       GAGAAGATAGCTGACCAGCTGGAAGAGTTGAATAAAGAGCTTACTGGAATCCAGCAGGGT
                  ************************************************************

HsCD00081592      TTTCTGCCCAAGGATTTGCAAGCTGAAGCTCTCTGCAAACTTGATAGGAGAGTAAAAGCC
NM_004323.5       TTTCTGCCCAAGGATTTGCAAGCTGAAGCTCTCTGCAAACTTGATAGGAGAGTAAAAGCC
                  ************************************************************

HsCD00081592      ACAATAGAGCAGTTTATGAAGATCTTGGAGGAGATTGACACACTGATCCTGCCAGAAAAT
NM_004323.5       ACAATAGAGCAGTTTATGAAGATCTTGGAGGAGATTGACACACTGATCCTGCCAGAAAAT
                  ************************************************************

HsCD00081592      TTCAAAGACAGTAGATTGAAAAGGAAAGGCTTGGTAAAAAAGGTTCAGGCATTCCTAGCC
NM_004323.5       TTCAAAGACAGTAGATTGAAAAGGAAAGGCTTGGTAAAAAAGGTTCAGGCATTCCTAGCC
                  ************************************************************

HsCD00081592      GAGTGTGACACAGTGGAGCAGAACATCTGCCAGGAGACTGAGCGGCTGCAGTCTACAAAC
NM_004323.5       GAGTGTGACACAGTGGAGCAGAACATCTGCCAGGAGACTGAGCGGCTGCAGTCTACAAAC
                  ************************************************************

HsCD00081592      TTTGCCCTGGCCGAG---
NM_004323.5       TTTGCCCTGGCCGAGTGA
                  ***************   

Pathways

NCI : AP-1 transcription factor network
WikiPathway : Androgen receptor signaling pathway

Clone

Cloning Information : 266216
HIP Master Clone ID : 198314
IMAGE ID : 100002016
Original Clone ID : FLH266216.01L

Organism


TAX_ID : 9606
Species Specific ID: 573

Genome

Chromosome : 9
Map Location : 9p12
Ensembl : ENSG00000107262

Reagents

Labome : Bag-1-antibody